Antibacterial activity of the marine sponge constituent cribrostatin 6

Robin Pettit, Bridget R. Fakoury, John C. Knight, Christine A. Weber, George Pettit, Gary D. Cage, Sandy Pon

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

The antibacterial activity of the nitrogen heterocyclic sponge constituent cribrostatin 6 was examined. Cribrostatin 6 was bacteriostatic for a variety of Gram-positive species and was bactericidal for the majority of clinical isolates of Streptococcus pneumoniae, including penicillin-resistant strains. Minimum bactericidal concentration/MIC ratios were ≤2 for 75 % of S. pneumoniae clinical isolates. Kill-curve analysis confirmed the bactericidal action of cribrostatin 6. Bactericidal activity was rather slow, beginning at 2, 4 or 8 h, depending on the strain. The frequency of occurrence of bacterial spontaneous mutations to resistance was ≤10-7. The maximum tolerated dose of cribrostatin 6 in mice was 750-1000 μg kg-1 day-1. Cribrostatin 6 is a promising lead antibiotic for Gram-positive bacteria, particularly S. pneumoniae, a leading cause of infection and mortality worldwide.

Original languageEnglish (US)
Pages (from-to)61-65
Number of pages5
JournalJournal of Medical Microbiology
Volume53
Issue number1
DOIs
StatePublished - Jan 2004

Fingerprint

Porifera
Streptococcus pneumoniae
Maximum Tolerated Dose
Gram-Positive Bacteria
Penicillins
Nitrogen
cribrostatin 6
Anti-Bacterial Agents
Mutation
Mortality
Infection

ASJC Scopus subject areas

  • Microbiology (medical)
  • Microbiology

Cite this

Antibacterial activity of the marine sponge constituent cribrostatin 6. / Pettit, Robin; Fakoury, Bridget R.; Knight, John C.; Weber, Christine A.; Pettit, George; Cage, Gary D.; Pon, Sandy.

In: Journal of Medical Microbiology, Vol. 53, No. 1, 01.2004, p. 61-65.

Research output: Contribution to journalArticle

Pettit, Robin ; Fakoury, Bridget R. ; Knight, John C. ; Weber, Christine A. ; Pettit, George ; Cage, Gary D. ; Pon, Sandy. / Antibacterial activity of the marine sponge constituent cribrostatin 6. In: Journal of Medical Microbiology. 2004 ; Vol. 53, No. 1. pp. 61-65.
@article{3a757d2b64354ceca8cc92f07fc04416,
title = "Antibacterial activity of the marine sponge constituent cribrostatin 6",
abstract = "The antibacterial activity of the nitrogen heterocyclic sponge constituent cribrostatin 6 was examined. Cribrostatin 6 was bacteriostatic for a variety of Gram-positive species and was bactericidal for the majority of clinical isolates of Streptococcus pneumoniae, including penicillin-resistant strains. Minimum bactericidal concentration/MIC ratios were ≤2 for 75 {\%} of S. pneumoniae clinical isolates. Kill-curve analysis confirmed the bactericidal action of cribrostatin 6. Bactericidal activity was rather slow, beginning at 2, 4 or 8 h, depending on the strain. The frequency of occurrence of bacterial spontaneous mutations to resistance was ≤10-7. The maximum tolerated dose of cribrostatin 6 in mice was 750-1000 μg kg-1 day-1. Cribrostatin 6 is a promising lead antibiotic for Gram-positive bacteria, particularly S. pneumoniae, a leading cause of infection and mortality worldwide.",
author = "Robin Pettit and Fakoury, {Bridget R.} and Knight, {John C.} and Weber, {Christine A.} and George Pettit and Cage, {Gary D.} and Sandy Pon",
year = "2004",
month = "1",
doi = "10.1099/jmm.0.05250-0",
language = "English (US)",
volume = "53",
pages = "61--65",
journal = "Journal of Medical Microbiology",
issn = "0022-2615",
publisher = "Society for General Microbiology",
number = "1",

}

TY - JOUR

T1 - Antibacterial activity of the marine sponge constituent cribrostatin 6

AU - Pettit, Robin

AU - Fakoury, Bridget R.

AU - Knight, John C.

AU - Weber, Christine A.

AU - Pettit, George

AU - Cage, Gary D.

AU - Pon, Sandy

PY - 2004/1

Y1 - 2004/1

N2 - The antibacterial activity of the nitrogen heterocyclic sponge constituent cribrostatin 6 was examined. Cribrostatin 6 was bacteriostatic for a variety of Gram-positive species and was bactericidal for the majority of clinical isolates of Streptococcus pneumoniae, including penicillin-resistant strains. Minimum bactericidal concentration/MIC ratios were ≤2 for 75 % of S. pneumoniae clinical isolates. Kill-curve analysis confirmed the bactericidal action of cribrostatin 6. Bactericidal activity was rather slow, beginning at 2, 4 or 8 h, depending on the strain. The frequency of occurrence of bacterial spontaneous mutations to resistance was ≤10-7. The maximum tolerated dose of cribrostatin 6 in mice was 750-1000 μg kg-1 day-1. Cribrostatin 6 is a promising lead antibiotic for Gram-positive bacteria, particularly S. pneumoniae, a leading cause of infection and mortality worldwide.

AB - The antibacterial activity of the nitrogen heterocyclic sponge constituent cribrostatin 6 was examined. Cribrostatin 6 was bacteriostatic for a variety of Gram-positive species and was bactericidal for the majority of clinical isolates of Streptococcus pneumoniae, including penicillin-resistant strains. Minimum bactericidal concentration/MIC ratios were ≤2 for 75 % of S. pneumoniae clinical isolates. Kill-curve analysis confirmed the bactericidal action of cribrostatin 6. Bactericidal activity was rather slow, beginning at 2, 4 or 8 h, depending on the strain. The frequency of occurrence of bacterial spontaneous mutations to resistance was ≤10-7. The maximum tolerated dose of cribrostatin 6 in mice was 750-1000 μg kg-1 day-1. Cribrostatin 6 is a promising lead antibiotic for Gram-positive bacteria, particularly S. pneumoniae, a leading cause of infection and mortality worldwide.

UR - http://www.scopus.com/inward/record.url?scp=1542635310&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=1542635310&partnerID=8YFLogxK

U2 - 10.1099/jmm.0.05250-0

DO - 10.1099/jmm.0.05250-0

M3 - Article

VL - 53

SP - 61

EP - 65

JO - Journal of Medical Microbiology

JF - Journal of Medical Microbiology

SN - 0022-2615

IS - 1

ER -