Analyzing Tau aggregation with electron microscopy

Carol J. Huseby, Jeff Kuret

Research output: Chapter in Book/Report/Conference proceedingChapter

7 Scopus citations

Abstract

Conversion of monomeric tau protein into filamentous aggregates is a defining event in the pathogenesis of Alzheimer's disease. To gain insight into disease pathogenesis, the mechanisms that trigger and mediate tau aggregation are under intense investigation. Characterization efforts have relied primarily on recombinant tau protein preparations and high-throughput solution-based detection methods such as thioflavindye fluorescence and laser-light-scattering spectroscopies. Transmission electron microscopy (TEM) is a static imaging tool that complements these approaches by detecting individual tau filaments at nanometer resolution. In doing so, it can provide unique insight into the quality, quantity, and composition of synthetic tau filament populations. Here we describe protocols for analysis of tau filament populations by TEM for purposes of dissecting aggregation mechanism.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages101-112
Number of pages12
DOIs
StatePublished - 2015
Externally publishedYes

Publication series

NameMethods in Molecular Biology
Volume1345
ISSN (Print)1064-3745

Keywords

  • Aggregation
  • Electron microscopy
  • Immunogold labeling
  • Kinetic analysis
  • Length distribution

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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