An FRET-based ratiometric chemosensor for in vitro cellular fluorescence analyses of pH

Xianfeng Zhou; Fengyu Su; Hongguang Lu; Patti Senechal-Willis; Yanqing Tian; Roger H. Johnson; Deirdre Meldrum

doi:10.1016/j.biomaterials.2011.09.053

An FRET-based ratiometric chemosensor for in vitro cellular fluorescence analyses of pH

Xianfeng Zhou, Fengyu Su, Hongguang Lu, Patti Senechal-Willis, Yanqing Tian, Roger H. Johnson, Deirdre Meldrum

Research output: Contribution to journal › Article › peer-review

159 Scopus citations

Abstract

Ratiometric fluorescence sensing is an important technique for precise and quantitative analysis of biological events occurring under complex conditions by simultaneously recording fluorescence intensities at two wavelengths and calculating their ratios. Herein, we design a ratiometric chemosensor for pH that is based on photo-induced electron transfer (PET) and binding-induced modulation of fluorescence resonance energy transfer (FRET) mechanisms. This ratiometric chemosensor was constructed by introduction of a pH-insensitive coumarin fluorophore as an FRET donor into a pH-sensitive amino-naphthalimide derivative as the FRET acceptor. The sensor exhibited clear dual-mission signal changes in blue and green spectral windows upon pH changes. The pH sensor was applied for not only measuring cellular pH, but also for visualizing stimulus-responsive changes of intracellular pH values.

Original language	English (US)
Pages (from-to)	171-180
Number of pages	10
Journal	Biomaterials
Volume	33
Issue number	1
DOIs	https://doi.org/10.1016/j.biomaterials.2011.09.053
State	Published - Jan 2012

Keywords

Biological imaging
FRET
PET
PH sensor
Ratiometric sensing

ASJC Scopus subject areas

Mechanics of Materials
Ceramics and Composites
Bioengineering
Biophysics
Biomaterials

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10.1016/j.biomaterials.2011.09.053

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title = "An FRET-based ratiometric chemosensor for in vitro cellular fluorescence analyses of pH",

abstract = "Ratiometric fluorescence sensing is an important technique for precise and quantitative analysis of biological events occurring under complex conditions by simultaneously recording fluorescence intensities at two wavelengths and calculating their ratios. Herein, we design a ratiometric chemosensor for pH that is based on photo-induced electron transfer (PET) and binding-induced modulation of fluorescence resonance energy transfer (FRET) mechanisms. This ratiometric chemosensor was constructed by introduction of a pH-insensitive coumarin fluorophore as an FRET donor into a pH-sensitive amino-naphthalimide derivative as the FRET acceptor. The sensor exhibited clear dual-mission signal changes in blue and green spectral windows upon pH changes. The pH sensor was applied for not only measuring cellular pH, but also for visualizing stimulus-responsive changes of intracellular pH values.",

keywords = "Biological imaging, FRET, PET, PH sensor, Ratiometric sensing",

author = "Xianfeng Zhou and Fengyu Su and Hongguang Lu and Patti Senechal-Willis and Yanqing Tian and Johnson, {Roger H.} and Deirdre Meldrum",

note = "Funding Information: Financial support was provided by the Microscale Life Sciences Center , a NIH Center of Excellence in Genomic Sciences at Arizona State University: Grant 5P50 HG002360, Dr. Deirdre Meldrum, PI, Director. Dr. Brian J. Reid and Dr. Tom Paulson at Fred Hutchison Cancer Research Center (Seattle, WA) were acknowledged for kindly providing us the CP-A cell line.",

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doi = "10.1016/j.biomaterials.2011.09.053",

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AU - Zhou, Xianfeng

AU - Su, Fengyu

AU - Lu, Hongguang

AU - Senechal-Willis, Patti

AU - Tian, Yanqing

AU - Johnson, Roger H.

AU - Meldrum, Deirdre

N1 - Funding Information: Financial support was provided by the Microscale Life Sciences Center , a NIH Center of Excellence in Genomic Sciences at Arizona State University: Grant 5P50 HG002360, Dr. Deirdre Meldrum, PI, Director. Dr. Brian J. Reid and Dr. Tom Paulson at Fred Hutchison Cancer Research Center (Seattle, WA) were acknowledged for kindly providing us the CP-A cell line.

PY - 2012/1

Y1 - 2012/1

N2 - Ratiometric fluorescence sensing is an important technique for precise and quantitative analysis of biological events occurring under complex conditions by simultaneously recording fluorescence intensities at two wavelengths and calculating their ratios. Herein, we design a ratiometric chemosensor for pH that is based on photo-induced electron transfer (PET) and binding-induced modulation of fluorescence resonance energy transfer (FRET) mechanisms. This ratiometric chemosensor was constructed by introduction of a pH-insensitive coumarin fluorophore as an FRET donor into a pH-sensitive amino-naphthalimide derivative as the FRET acceptor. The sensor exhibited clear dual-mission signal changes in blue and green spectral windows upon pH changes. The pH sensor was applied for not only measuring cellular pH, but also for visualizing stimulus-responsive changes of intracellular pH values.

AB - Ratiometric fluorescence sensing is an important technique for precise and quantitative analysis of biological events occurring under complex conditions by simultaneously recording fluorescence intensities at two wavelengths and calculating their ratios. Herein, we design a ratiometric chemosensor for pH that is based on photo-induced electron transfer (PET) and binding-induced modulation of fluorescence resonance energy transfer (FRET) mechanisms. This ratiometric chemosensor was constructed by introduction of a pH-insensitive coumarin fluorophore as an FRET donor into a pH-sensitive amino-naphthalimide derivative as the FRET acceptor. The sensor exhibited clear dual-mission signal changes in blue and green spectral windows upon pH changes. The pH sensor was applied for not only measuring cellular pH, but also for visualizing stimulus-responsive changes of intracellular pH values.

KW - Biological imaging

KW - FRET

KW - PET

KW - PH sensor

KW - Ratiometric sensing

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An FRET-based ratiometric chemosensor for in vitro cellular fluorescence analyses of pH

Abstract

Keywords

ASJC Scopus subject areas

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