TY - GEN
T1 - Amplification circuit and microelectrode array for HL-1 cardiomyocyte action potential measurement
AU - Song, Jianan
AU - Welch, David
AU - Blain Christen, Jennifer
PY - 2010
Y1 - 2010
N2 - We have designed and tested two amplification circuits for measuring HL-1 cardiac action potentials, single-ended and differential. We describe the improvements in performance resulting from the differential configuration. These improvements are especially important considering the cell culture's aqueous environment that experiences DC drift. In addition we have designed and fabricated a 4X4 gold microelectrode array and cultured HL-1 cells on the electrodes. The array is connected to our circuit through a custom made Pogo pin jig to allow for easy measurements from the electrodes while cells are being cultured. This setup allowed us to obtain initial results for modeling the transfer function of the cell culture as a passive device network. Testing has confirmed the circuits are capable of filtering low frequency signals including DC drift and frequencies above 5 kHz. The amplification was achieved through two gain stages with a total gain of 60 dB for the single-ended and 46 dB for the differential circuits.
AB - We have designed and tested two amplification circuits for measuring HL-1 cardiac action potentials, single-ended and differential. We describe the improvements in performance resulting from the differential configuration. These improvements are especially important considering the cell culture's aqueous environment that experiences DC drift. In addition we have designed and fabricated a 4X4 gold microelectrode array and cultured HL-1 cells on the electrodes. The array is connected to our circuit through a custom made Pogo pin jig to allow for easy measurements from the electrodes while cells are being cultured. This setup allowed us to obtain initial results for modeling the transfer function of the cell culture as a passive device network. Testing has confirmed the circuits are capable of filtering low frequency signals including DC drift and frequencies above 5 kHz. The amplification was achieved through two gain stages with a total gain of 60 dB for the single-ended and 46 dB for the differential circuits.
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U2 - 10.1109/ISCAS.2010.5537259
DO - 10.1109/ISCAS.2010.5537259
M3 - Conference contribution
AN - SCOPUS:77956004187
SN - 9781424453085
T3 - ISCAS 2010 - 2010 IEEE International Symposium on Circuits and Systems: Nano-Bio Circuit Fabrics and Systems
SP - 1384
EP - 1387
BT - ISCAS 2010 - 2010 IEEE International Symposium on Circuits and Systems
T2 - 2010 IEEE International Symposium on Circuits and Systems: Nano-Bio Circuit Fabrics and Systems, ISCAS 2010
Y2 - 30 May 2010 through 2 June 2010
ER -