Amplification circuit and microelectrode array for HL-1 cardiomyocyte action potential measurement

Jianan Song, David Welch, Jennifer Blain Christen

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Scopus citations

Abstract

We have designed and tested two amplification circuits for measuring HL-1 cardiac action potentials, single-ended and differential. We describe the improvements in performance resulting from the differential configuration. These improvements are especially important considering the cell culture's aqueous environment that experiences DC drift. In addition we have designed and fabricated a 4X4 gold microelectrode array and cultured HL-1 cells on the electrodes. The array is connected to our circuit through a custom made Pogo pin jig to allow for easy measurements from the electrodes while cells are being cultured. This setup allowed us to obtain initial results for modeling the transfer function of the cell culture as a passive device network. Testing has confirmed the circuits are capable of filtering low frequency signals including DC drift and frequencies above 5 kHz. The amplification was achieved through two gain stages with a total gain of 60 dB for the single-ended and 46 dB for the differential circuits.

Original languageEnglish (US)
Title of host publicationISCAS 2010 - 2010 IEEE International Symposium on Circuits and Systems
Subtitle of host publicationNano-Bio Circuit Fabrics and Systems
Pages1384-1387
Number of pages4
DOIs
StatePublished - Aug 31 2010
Event2010 IEEE International Symposium on Circuits and Systems: Nano-Bio Circuit Fabrics and Systems, ISCAS 2010 - Paris, France
Duration: May 30 2010Jun 2 2010

Publication series

NameISCAS 2010 - 2010 IEEE International Symposium on Circuits and Systems: Nano-Bio Circuit Fabrics and Systems

Other

Other2010 IEEE International Symposium on Circuits and Systems: Nano-Bio Circuit Fabrics and Systems, ISCAS 2010
CountryFrance
CityParis
Period5/30/106/2/10

ASJC Scopus subject areas

  • Hardware and Architecture
  • Electrical and Electronic Engineering

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    Song, J., Welch, D., & Blain Christen, J. (2010). Amplification circuit and microelectrode array for HL-1 cardiomyocyte action potential measurement. In ISCAS 2010 - 2010 IEEE International Symposium on Circuits and Systems: Nano-Bio Circuit Fabrics and Systems (pp. 1384-1387). [5537259] (ISCAS 2010 - 2010 IEEE International Symposium on Circuits and Systems: Nano-Bio Circuit Fabrics and Systems). https://doi.org/10.1109/ISCAS.2010.5537259