Altered sphingolipid function in Alzheimer's disease; a gene regulatory network approach

Caterina Giovagnoni, Muhammad Ali, Lars M.T. Eijssen, Richard Maes, Kyonghwan Choe, Monique Mulder, Jos Kleinjans, Antonio del Sol, Enrico Glaab, Diego Mastroeni, Elaine Delvaux, Paul Coleman, Mario Losen, Ehsan Pishva, Pilar Martinez-Martinez, Daniel L.A. van den Hove

Research output: Contribution to journalArticlepeer-review

Abstract

Sphingolipids (SLs) are bioactive lipids involved in various important physiological functions. The SL pathway has been shown to be affected in several brain-related disorders, including Alzheimer's disease (AD). Recent evidence suggests that epigenetic dysregulation plays an important role in the pathogenesis of AD as well. Here, we use an integrative approach to better understand the relationship between epigenetic and transcriptomic processes in regulating SL function in the middle temporal gyrus of AD patients. Transcriptomic analysis of 252 SL-related genes, selected based on GO term annotations, from 46 AD patients and 32 healthy age-matched controls, revealed 103 differentially expressed SL-related genes in AD patients. Additionally, methylomic analysis of the same subjects revealed parallel hydroxymethylation changes in PTGIS, GBA, and ITGB2 in AD. Subsequent gene regulatory network-based analysis identified 3 candidate genes, that is, SELPLG, SPHK1 and CAV1 whose alteration holds the potential to revert the gene expression program from a diseased towards a healthy state. Together, this epigenomic and transcriptomic approach highlights the importance of SL-related genes in AD, and may provide novel biomarkers and therapeutic alternatives to traditionally investigated biological pathways in AD.

Original languageEnglish (US)
Pages (from-to)178-187
Number of pages10
JournalNeurobiology of Aging
Volume102
DOIs
StatePublished - Jun 2021

Keywords

  • Alzheimer's disease
  • Disease network analysis
  • Epigenetics
  • Gene regulatory network
  • Sphingolipids

ASJC Scopus subject areas

  • Neuroscience(all)
  • Aging
  • Developmental Biology
  • Clinical Neurology
  • Geriatrics and Gerontology

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