TY - JOUR
T1 - Altered sphingolipid function in Alzheimer's disease; a gene regulatory network approach
AU - Giovagnoni, Caterina
AU - Ali, Muhammad
AU - Eijssen, Lars M.T.
AU - Maes, Richard
AU - Choe, Kyonghwan
AU - Mulder, Monique
AU - Kleinjans, Jos
AU - del Sol, Antonio
AU - Glaab, Enrico
AU - Mastroeni, Diego
AU - Delvaux, Elaine
AU - Coleman, Paul
AU - Losen, Mario
AU - Pishva, Ehsan
AU - Martinez-Martinez, Pilar
AU - van den Hove, Daniel L.A.
N1 - Publisher Copyright:
© 2021
PY - 2021/6
Y1 - 2021/6
N2 - Sphingolipids (SLs) are bioactive lipids involved in various important physiological functions. The SL pathway has been shown to be affected in several brain-related disorders, including Alzheimer's disease (AD). Recent evidence suggests that epigenetic dysregulation plays an important role in the pathogenesis of AD as well. Here, we use an integrative approach to better understand the relationship between epigenetic and transcriptomic processes in regulating SL function in the middle temporal gyrus of AD patients. Transcriptomic analysis of 252 SL-related genes, selected based on GO term annotations, from 46 AD patients and 32 healthy age-matched controls, revealed 103 differentially expressed SL-related genes in AD patients. Additionally, methylomic analysis of the same subjects revealed parallel hydroxymethylation changes in PTGIS, GBA, and ITGB2 in AD. Subsequent gene regulatory network-based analysis identified 3 candidate genes, that is, SELPLG, SPHK1 and CAV1 whose alteration holds the potential to revert the gene expression program from a diseased towards a healthy state. Together, this epigenomic and transcriptomic approach highlights the importance of SL-related genes in AD, and may provide novel biomarkers and therapeutic alternatives to traditionally investigated biological pathways in AD.
AB - Sphingolipids (SLs) are bioactive lipids involved in various important physiological functions. The SL pathway has been shown to be affected in several brain-related disorders, including Alzheimer's disease (AD). Recent evidence suggests that epigenetic dysregulation plays an important role in the pathogenesis of AD as well. Here, we use an integrative approach to better understand the relationship between epigenetic and transcriptomic processes in regulating SL function in the middle temporal gyrus of AD patients. Transcriptomic analysis of 252 SL-related genes, selected based on GO term annotations, from 46 AD patients and 32 healthy age-matched controls, revealed 103 differentially expressed SL-related genes in AD patients. Additionally, methylomic analysis of the same subjects revealed parallel hydroxymethylation changes in PTGIS, GBA, and ITGB2 in AD. Subsequent gene regulatory network-based analysis identified 3 candidate genes, that is, SELPLG, SPHK1 and CAV1 whose alteration holds the potential to revert the gene expression program from a diseased towards a healthy state. Together, this epigenomic and transcriptomic approach highlights the importance of SL-related genes in AD, and may provide novel biomarkers and therapeutic alternatives to traditionally investigated biological pathways in AD.
KW - Alzheimer's disease
KW - Disease network analysis
KW - Epigenetics
KW - Gene regulatory network
KW - Sphingolipids
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U2 - 10.1016/j.neurobiolaging.2021.02.001
DO - 10.1016/j.neurobiolaging.2021.02.001
M3 - Article
C2 - 33773368
AN - SCOPUS:85103093228
SN - 0197-4580
VL - 102
SP - 178
EP - 187
JO - Neurobiology of Aging
JF - Neurobiology of Aging
ER -