Addressing the diversity of the honeybee gut symbiont Gilliamella

Description of Gilliamella apis sp. nov., isolated from the gut of honeybees (Apis mellifera)

Jane Ludvigsen, Davide Porcellato, Gro Amdam, Knut Rudi

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

The gut microbiota of honeybees (Apis) and bumblebees (Bombus) include the symbiotic bacterial genus Gilliamella. This genus shows a high degree of functional and genomic diversity and separates into distinct lineages. Gilliamella apicola wkB1T, which was isolated from Apis, was the first species to be described. Recently four new species, isolated from Bombus, were identified. In this paper, we compare several genomes/strains from previous studies spanning this diversity, which gives insight into the phylogenetic relationship among different Gilliamella species. We show that one lineage, isolated only from Apis, is different from other gilliamellas described, based on average nucleotide identity calculation (about 80 %) and phenotypic characterizations. We propose the new species name for this lineage: Gilliamella apis sp. nov. We present the characterization of the type strain NO3T (=DSM 105629T=LMG 30293T), a strain isolated from the Western honeybee Apis mellifera, which clusters within this lineage. Cells of strain NO3T grow best in a microaerophilic atmosphere with enhanced CO2 levels at 36 ºC and pH 7.0-7.5. Cells also grow well in anaerobic conditions, but not in aerobic conditions. Cells are approximately 1μm in length and rod-shaped, and the genomic G+C content is 34.7 mol%. Differential characteristics between strain NO3T and the different type strains of Gilliamella were revealed based on API kit tests and genomic content comparisons. The main respiratory quinone of strain NO3T was ubiquinone-8, and the predominant fatty acids were C18: 1ω7c/C18: 1ω6c, C16: 0, consistent with the genus Gilliamella.

Original languageEnglish (US)
Article number002749
Pages (from-to)1762-1770
Number of pages9
JournalInternational Journal of Systematic and Evolutionary Microbiology
Volume68
Issue number5
DOIs
StatePublished - May 1 2018

Fingerprint

Apis
honeybee
Bees
symbiont
Apis mellifera
symbionts
honey bees
genomics
digestive system
new species
Bombus
oxic conditions
anoxic conditions
fatty acid
genome
phylogenetics
Base Composition
Atmosphere
atmosphere
Names

Keywords

  • Genome phylogeny
  • Gilliamella apis NO3
  • Honeybee gut symbiont

ASJC Scopus subject areas

  • Microbiology
  • Ecology, Evolution, Behavior and Systematics

Cite this

@article{43203d18eb9c4957b34374fc772e3161,
title = "Addressing the diversity of the honeybee gut symbiont Gilliamella: Description of Gilliamella apis sp. nov., isolated from the gut of honeybees (Apis mellifera)",
abstract = "The gut microbiota of honeybees (Apis) and bumblebees (Bombus) include the symbiotic bacterial genus Gilliamella. This genus shows a high degree of functional and genomic diversity and separates into distinct lineages. Gilliamella apicola wkB1T, which was isolated from Apis, was the first species to be described. Recently four new species, isolated from Bombus, were identified. In this paper, we compare several genomes/strains from previous studies spanning this diversity, which gives insight into the phylogenetic relationship among different Gilliamella species. We show that one lineage, isolated only from Apis, is different from other gilliamellas described, based on average nucleotide identity calculation (about 80 {\%}) and phenotypic characterizations. We propose the new species name for this lineage: Gilliamella apis sp. nov. We present the characterization of the type strain NO3T (=DSM 105629T=LMG 30293T), a strain isolated from the Western honeybee Apis mellifera, which clusters within this lineage. Cells of strain NO3T grow best in a microaerophilic atmosphere with enhanced CO2 levels at 36 ºC and pH 7.0-7.5. Cells also grow well in anaerobic conditions, but not in aerobic conditions. Cells are approximately 1μm in length and rod-shaped, and the genomic G+C content is 34.7 mol{\%}. Differential characteristics between strain NO3T and the different type strains of Gilliamella were revealed based on API kit tests and genomic content comparisons. The main respiratory quinone of strain NO3T was ubiquinone-8, and the predominant fatty acids were C18: 1ω7c/C18: 1ω6c, C16: 0, consistent with the genus Gilliamella.",
keywords = "Genome phylogeny, Gilliamella apis NO3, Honeybee gut symbiont",
author = "Jane Ludvigsen and Davide Porcellato and Gro Amdam and Knut Rudi",
year = "2018",
month = "5",
day = "1",
doi = "10.1099/ijsem.0.002749",
language = "English (US)",
volume = "68",
pages = "1762--1770",
journal = "International Journal of Systematic and Evolutionary Microbiology",
issn = "1466-5026",
publisher = "Society for General Microbiology",
number = "5",

}

TY - JOUR

T1 - Addressing the diversity of the honeybee gut symbiont Gilliamella

T2 - Description of Gilliamella apis sp. nov., isolated from the gut of honeybees (Apis mellifera)

AU - Ludvigsen, Jane

AU - Porcellato, Davide

AU - Amdam, Gro

AU - Rudi, Knut

PY - 2018/5/1

Y1 - 2018/5/1

N2 - The gut microbiota of honeybees (Apis) and bumblebees (Bombus) include the symbiotic bacterial genus Gilliamella. This genus shows a high degree of functional and genomic diversity and separates into distinct lineages. Gilliamella apicola wkB1T, which was isolated from Apis, was the first species to be described. Recently four new species, isolated from Bombus, were identified. In this paper, we compare several genomes/strains from previous studies spanning this diversity, which gives insight into the phylogenetic relationship among different Gilliamella species. We show that one lineage, isolated only from Apis, is different from other gilliamellas described, based on average nucleotide identity calculation (about 80 %) and phenotypic characterizations. We propose the new species name for this lineage: Gilliamella apis sp. nov. We present the characterization of the type strain NO3T (=DSM 105629T=LMG 30293T), a strain isolated from the Western honeybee Apis mellifera, which clusters within this lineage. Cells of strain NO3T grow best in a microaerophilic atmosphere with enhanced CO2 levels at 36 ºC and pH 7.0-7.5. Cells also grow well in anaerobic conditions, but not in aerobic conditions. Cells are approximately 1μm in length and rod-shaped, and the genomic G+C content is 34.7 mol%. Differential characteristics between strain NO3T and the different type strains of Gilliamella were revealed based on API kit tests and genomic content comparisons. The main respiratory quinone of strain NO3T was ubiquinone-8, and the predominant fatty acids were C18: 1ω7c/C18: 1ω6c, C16: 0, consistent with the genus Gilliamella.

AB - The gut microbiota of honeybees (Apis) and bumblebees (Bombus) include the symbiotic bacterial genus Gilliamella. This genus shows a high degree of functional and genomic diversity and separates into distinct lineages. Gilliamella apicola wkB1T, which was isolated from Apis, was the first species to be described. Recently four new species, isolated from Bombus, were identified. In this paper, we compare several genomes/strains from previous studies spanning this diversity, which gives insight into the phylogenetic relationship among different Gilliamella species. We show that one lineage, isolated only from Apis, is different from other gilliamellas described, based on average nucleotide identity calculation (about 80 %) and phenotypic characterizations. We propose the new species name for this lineage: Gilliamella apis sp. nov. We present the characterization of the type strain NO3T (=DSM 105629T=LMG 30293T), a strain isolated from the Western honeybee Apis mellifera, which clusters within this lineage. Cells of strain NO3T grow best in a microaerophilic atmosphere with enhanced CO2 levels at 36 ºC and pH 7.0-7.5. Cells also grow well in anaerobic conditions, but not in aerobic conditions. Cells are approximately 1μm in length and rod-shaped, and the genomic G+C content is 34.7 mol%. Differential characteristics between strain NO3T and the different type strains of Gilliamella were revealed based on API kit tests and genomic content comparisons. The main respiratory quinone of strain NO3T was ubiquinone-8, and the predominant fatty acids were C18: 1ω7c/C18: 1ω6c, C16: 0, consistent with the genus Gilliamella.

KW - Genome phylogeny

KW - Gilliamella apis NO3

KW - Honeybee gut symbiont

UR - http://www.scopus.com/inward/record.url?scp=85046534602&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85046534602&partnerID=8YFLogxK

U2 - 10.1099/ijsem.0.002749

DO - 10.1099/ijsem.0.002749

M3 - Article

VL - 68

SP - 1762

EP - 1770

JO - International Journal of Systematic and Evolutionary Microbiology

JF - International Journal of Systematic and Evolutionary Microbiology

SN - 1466-5026

IS - 5

M1 - 002749

ER -