Addressing the diversity of the honeybee gut symbiont Gilliamella: Description of Gilliamella apis sp. nov., isolated from the gut of honeybees (Apis mellifera)

Jane Ludvigsen, Davide Porcellato, Gro Amdam, Knut Rudi

    Research output: Contribution to journalArticle

    2 Citations (Scopus)

    Abstract

    The gut microbiota of honeybees (Apis) and bumblebees (Bombus) include the symbiotic bacterial genus Gilliamella. This genus shows a high degree of functional and genomic diversity and separates into distinct lineages. Gilliamella apicola wkB1T, which was isolated from Apis, was the first species to be described. Recently four new species, isolated from Bombus, were identified. In this paper, we compare several genomes/strains from previous studies spanning this diversity, which gives insight into the phylogenetic relationship among different Gilliamella species. We show that one lineage, isolated only from Apis, is different from other gilliamellas described, based on average nucleotide identity calculation (about 80 %) and phenotypic characterizations. We propose the new species name for this lineage: Gilliamella apis sp. nov. We present the characterization of the type strain NO3T (=DSM 105629T=LMG 30293T), a strain isolated from the Western honeybee Apis mellifera, which clusters within this lineage. Cells of strain NO3T grow best in a microaerophilic atmosphere with enhanced CO2 levels at 36 ºC and pH 7.0-7.5. Cells also grow well in anaerobic conditions, but not in aerobic conditions. Cells are approximately 1μm in length and rod-shaped, and the genomic G+C content is 34.7 mol%. Differential characteristics between strain NO3T and the different type strains of Gilliamella were revealed based on API kit tests and genomic content comparisons. The main respiratory quinone of strain NO3T was ubiquinone-8, and the predominant fatty acids were C18: 1ω7c/C18: 1ω6c, C16: 0, consistent with the genus Gilliamella.

    Original languageEnglish (US)
    Article number002749
    Pages (from-to)1762-1770
    Number of pages9
    JournalInternational Journal of Systematic and Evolutionary Microbiology
    Volume68
    Issue number5
    DOIs
    StatePublished - May 1 2018

    Fingerprint

    Apis
    honeybee
    Bees
    symbiont
    Apis mellifera
    symbionts
    honey bees
    genomics
    digestive system
    new species
    Bombus
    oxic conditions
    anoxic conditions
    fatty acid
    genome
    phylogenetics
    Base Composition
    Atmosphere
    atmosphere
    Names

    Keywords

    • Genome phylogeny
    • Gilliamella apis NO3
    • Honeybee gut symbiont

    ASJC Scopus subject areas

    • Microbiology
    • Ecology, Evolution, Behavior and Systematics

    Cite this

    @article{43203d18eb9c4957b34374fc772e3161,
    title = "Addressing the diversity of the honeybee gut symbiont Gilliamella: Description of Gilliamella apis sp. nov., isolated from the gut of honeybees (Apis mellifera)",
    abstract = "The gut microbiota of honeybees (Apis) and bumblebees (Bombus) include the symbiotic bacterial genus Gilliamella. This genus shows a high degree of functional and genomic diversity and separates into distinct lineages. Gilliamella apicola wkB1T, which was isolated from Apis, was the first species to be described. Recently four new species, isolated from Bombus, were identified. In this paper, we compare several genomes/strains from previous studies spanning this diversity, which gives insight into the phylogenetic relationship among different Gilliamella species. We show that one lineage, isolated only from Apis, is different from other gilliamellas described, based on average nucleotide identity calculation (about 80 {\%}) and phenotypic characterizations. We propose the new species name for this lineage: Gilliamella apis sp. nov. We present the characterization of the type strain NO3T (=DSM 105629T=LMG 30293T), a strain isolated from the Western honeybee Apis mellifera, which clusters within this lineage. Cells of strain NO3T grow best in a microaerophilic atmosphere with enhanced CO2 levels at 36 ºC and pH 7.0-7.5. Cells also grow well in anaerobic conditions, but not in aerobic conditions. Cells are approximately 1μm in length and rod-shaped, and the genomic G+C content is 34.7 mol{\%}. Differential characteristics between strain NO3T and the different type strains of Gilliamella were revealed based on API kit tests and genomic content comparisons. The main respiratory quinone of strain NO3T was ubiquinone-8, and the predominant fatty acids were C18: 1ω7c/C18: 1ω6c, C16: 0, consistent with the genus Gilliamella.",
    keywords = "Genome phylogeny, Gilliamella apis NO3, Honeybee gut symbiont",
    author = "Jane Ludvigsen and Davide Porcellato and Gro Amdam and Knut Rudi",
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    doi = "10.1099/ijsem.0.002749",
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    T2 - Description of Gilliamella apis sp. nov., isolated from the gut of honeybees (Apis mellifera)

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    AU - Porcellato, Davide

    AU - Amdam, Gro

    AU - Rudi, Knut

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