Abstract
We studied whether voluntary running in an activity wheel moderates splenic natural killer (NK) cell cytotoxicity after footshock. Young (50- day) male Fischer 344 rats were randomly assigned to 1) sedentary (n = 16) or 2) activity-wheel (n = 16) groups that each received controllable or uncontrollable footshock on 2 consecutive days or 3) a sedentary home-cage control group (n = 8). Spleens and trunk blood were collected 30 min after the second footshock session. Cytotoxicity was determined by a standard 4-h 51Cr release assay. Percentages of OX6+ (B), OX8+ [T suppressor/cytotoxic (T(s/c)], W3/25+ (T helper), Thy-1.1 (Pan T cell marker), and 5C6+ (NK) cells were determined by flow cytometry. Plasma adrenocorticotropic hormone, corticosterone, and prolactin concentrations were measured by radioimmunoassay as modulators of NK activity. Percentage of specific lysis after footshock was ~52% of control values for sedentary animals compared with ~96% of control values for activity-wheel animals. The groups did not differ in percentages of NK or T(s/c) cells. We conclude that voluntary activity-wheel running protects against the suppression of splenic NK activity induced by footshock. This protective effect of wheel running is not explained by an elevation in baseline NK activity; increased percentages of splenic NK or T(s/c) cells; or plasma levels of adrenocorticotropic hormone, corticosterone, and prolactin.
Original language | English (US) |
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Pages (from-to) | 1547-1554 |
Number of pages | 8 |
Journal | Journal of Applied Physiology |
Volume | 78 |
Issue number | 4 |
DOIs | |
State | Published - 1995 |
Externally published | Yes |
Keywords
- adrenocorticotropic hormone
- corticosterone
- lymphocytes
- prolactin
- rat model
ASJC Scopus subject areas
- Physiology
- Physiology (medical)