Abstract
Neuronal adhesion plays a fundamental role In growth, migration, regeneration and plasticity of neurons. However, current methods for studying cellular adhesion cannot monitor the process in real time. We used quartz crystal microbalance (QCM) to measure adhesion of neuro- blastoma cells. In-vitro experiments were performed with approximately 6000 neuro-blastoma cells. The quartz crystal sandwiched between gold electrodes was placed in a flow-cell and filled with 600 μl of phosphate buffered solution (PBS). Addition of 10 μl of media and cells into the above set-up caused the resonant frequency of the quartz crystal to decrease by approximately 5000 Hz over a period of 6 hours. Control experiments with no cells elicited no change in the resonant frequency of the quartz crystal. The linearity of the response of the sensor was confirmed in experiments done with different number of cells. We conclude that the acoustic sensor can be a very sensitive indicator and can potentially be used for monitoring the neuronal adhesion process In real-time.
| Original language | English (US) |
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| Title of host publication | Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings |
| Editors | R.S. Leder |
| Pages | 2186-2188 |
| Number of pages | 3 |
| Volume | 3 |
| State | Published - 2003 |
| Event | A New Beginning for Human Health: Proceedings of the 25th Annual International Conference of the IEEE Engineering in Medicine and Biology Society - Cancun, Mexico Duration: Sep 17 2003 → Sep 21 2003 |
Other
| Other | A New Beginning for Human Health: Proceedings of the 25th Annual International Conference of the IEEE Engineering in Medicine and Biology Society |
|---|---|
| Country/Territory | Mexico |
| City | Cancun |
| Period | 9/17/03 → 9/21/03 |
Keywords
- Cell adhesion
- Neuronal adhesion
- QCM
- Real time
ASJC Scopus subject areas
- Bioengineering