Acoustic sensors for monitoring neuronal adhesion in real-time

M. L. Khraiche, A. Zhou, Jitendran Muthuswamy

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Scopus citations

Abstract

Neuronal adhesion plays a fundamental role In growth, migration, regeneration and plasticity of neurons. However, current methods for studying cellular adhesion cannot monitor the process in real time. We used quartz crystal microbalance (QCM) to measure adhesion of neuro- blastoma cells. In-vitro experiments were performed with approximately 6000 neuro-blastoma cells. The quartz crystal sandwiched between gold electrodes was placed in a flow-cell and filled with 600 μl of phosphate buffered solution (PBS). Addition of 10 μl of media and cells into the above set-up caused the resonant frequency of the quartz crystal to decrease by approximately 5000 Hz over a period of 6 hours. Control experiments with no cells elicited no change in the resonant frequency of the quartz crystal. The linearity of the response of the sensor was confirmed in experiments done with different number of cells. We conclude that the acoustic sensor can be a very sensitive indicator and can potentially be used for monitoring the neuronal adhesion process In real-time.

Original languageEnglish (US)
Title of host publicationAnnual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings
EditorsR.S. Leder
Pages2186-2188
Number of pages3
Volume3
StatePublished - 2003
EventA New Beginning for Human Health: Proceedings of the 25th Annual International Conference of the IEEE Engineering in Medicine and Biology Society - Cancun, Mexico
Duration: Sep 17 2003Sep 21 2003

Other

OtherA New Beginning for Human Health: Proceedings of the 25th Annual International Conference of the IEEE Engineering in Medicine and Biology Society
CountryMexico
CityCancun
Period9/17/039/21/03

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Keywords

  • Cell adhesion
  • Neuronal adhesion
  • QCM
  • Real time

ASJC Scopus subject areas

  • Bioengineering

Cite this

Khraiche, M. L., Zhou, A., & Muthuswamy, J. (2003). Acoustic sensors for monitoring neuronal adhesion in real-time. In R. S. Leder (Ed.), Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings (Vol. 3, pp. 2186-2188)