Acid-fast staining and Petroff-Hausser chamber counting of mycobacterial cells in liquid suspension

Robin Treuer, Shelley Haydel

Research output: Contribution to journalArticle

10 Scopus citations


Accurate and rapid cell counts of mycobacterial species in culture are difficult to obtain. Here, a method using modified Kinyoun acid-fast staining was adapted for use with a Petroff-Hausser sperm and bacteria cell-counting chamber by using a liquid suspension staining technique. Cell counts obtained by this method were compared to viable cell counts by agar plate counting, revealing accurate correlation.

Original languageEnglish (US)
Article number10A.6
JournalCurrent Protocols in Microbiology
Issue numberSUPPL.20
StatePublished - Feb 1 2011



  • Acid-fast staining
  • Hemacytometer
  • Microscope
  • Mycobacteria

ASJC Scopus subject areas

  • Parasitology
  • Microbiology
  • Virology

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