Abstract
Accurate and rapid cell counts of mycobacterial species in culture are difficult to obtain. Here, a method using modified Kinyoun acid-fast staining was adapted for use with a Petroff-Hausser sperm and bacteria cell-counting chamber by using a liquid suspension staining technique. Cell counts obtained by this method were compared to viable cell counts by agar plate counting, revealing accurate correlation.
Original language | English (US) |
---|---|
Article number | 10A.6 |
Journal | Current Protocols in Microbiology |
Issue number | SUPPL.20 |
DOIs | |
State | Published - Feb 2011 |
Keywords
- Acid-fast staining
- Hemacytometer
- Microscope
- Mycobacteria
ASJC Scopus subject areas
- Parasitology
- Microbiology
- Virology