A strong signature of balancing selection in the 5′ cis-regulatory region of CCR5

Michael J. Bamshad, Srinivas Mummidi, Enrique Gonzalez, Seema S. Ahuja, Diane M. Dunn, W. Scott Watkins, Stephen Wooding, Anne Stone, Lynn B. Jorde, Robert B. Weiss, Sunil K. Ahuja

Research output: Contribution to journalArticle

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Abstract

CCR5 encodes a cell surface chemokine receptor molecule that serves as the principal coreceptor, with CD4, for HIV-type 1 (HIV-1). Varied HIV-1 susceptibility and time to progression to AIDS have been associated with polymorphisms in CCR5. Many of these polymorphisms are located in the 5′ cis-regulatory region of CCR5, suggesting that it may have been a target of natural selection. We characterized CCR5 sequence variation in this region in 400 chromosomes from worldwide populations and compared it to a genome-wide analysis of 100 Alu polymorphisms typed in the same populations. Variation was substantially higher than expected and characterized by an excess of intermediate-frequency alleles. A genealogy of CCR5 haplotypes had deep branch lengths despite markedly little differentiation among populations. This finding suggested a deviation from neutrality not accounted for by population structure, which was confirmed by tests for natural selection. These results are strong evidence that balancing selection has shaped the pattern of variation in CCR5 and suggest that HIV-1 resistance afforded by CCR5 5′ cis-regulatory region haplotypes may be the consequence of adaptive changes to older pathogens.

Original languageEnglish (US)
Pages (from-to)10539-10544
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume99
Issue number16
DOIs
StatePublished - Aug 2002
Externally publishedYes

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Nucleic Acid Regulatory Sequences
HIV-1
Genetic Selection
Haplotypes
Population
Genealogy and Heraldry
Chemokine Receptors
Cell Surface Receptors
Gene Frequency
Acquired Immunodeficiency Syndrome
Chromosomes
Genome

ASJC Scopus subject areas

  • Genetics
  • General

Cite this

Bamshad, M. J., Mummidi, S., Gonzalez, E., Ahuja, S. S., Dunn, D. M., Watkins, W. S., ... Ahuja, S. K. (2002). A strong signature of balancing selection in the 5′ cis-regulatory region of CCR5. Proceedings of the National Academy of Sciences of the United States of America, 99(16), 10539-10544. https://doi.org/10.1073/pnas.162046399

A strong signature of balancing selection in the 5′ cis-regulatory region of CCR5. / Bamshad, Michael J.; Mummidi, Srinivas; Gonzalez, Enrique; Ahuja, Seema S.; Dunn, Diane M.; Watkins, W. Scott; Wooding, Stephen; Stone, Anne; Jorde, Lynn B.; Weiss, Robert B.; Ahuja, Sunil K.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 99, No. 16, 08.2002, p. 10539-10544.

Research output: Contribution to journalArticle

Bamshad, MJ, Mummidi, S, Gonzalez, E, Ahuja, SS, Dunn, DM, Watkins, WS, Wooding, S, Stone, A, Jorde, LB, Weiss, RB & Ahuja, SK 2002, 'A strong signature of balancing selection in the 5′ cis-regulatory region of CCR5', Proceedings of the National Academy of Sciences of the United States of America, vol. 99, no. 16, pp. 10539-10544. https://doi.org/10.1073/pnas.162046399
Bamshad, Michael J. ; Mummidi, Srinivas ; Gonzalez, Enrique ; Ahuja, Seema S. ; Dunn, Diane M. ; Watkins, W. Scott ; Wooding, Stephen ; Stone, Anne ; Jorde, Lynn B. ; Weiss, Robert B. ; Ahuja, Sunil K. / A strong signature of balancing selection in the 5′ cis-regulatory region of CCR5. In: Proceedings of the National Academy of Sciences of the United States of America. 2002 ; Vol. 99, No. 16. pp. 10539-10544.
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