A Simple Platform for the Rapid Development of Antimicrobials

Stephen Johnston, Valeriy Domenyuk, Nidhi Gupta, Milene Tavares Batista, John C. Lainson, Zhan-Gong Zhao, Joel F. Lusk, Andrey Loskutov, Zbigniew Cichacz, Phillip Stafford, Joseph Barten Legutki, Chris Diehnelt

Research output: Contribution to journalArticle

Abstract

Recent infectious outbreaks highlight the need for platform technologies that can be quickly deployed to develop therapeutics needed to contain the outbreak. We present a simple concept for rapid development of new antimicrobials. The goal was to produce in as little as one week thousands of doses of an intervention for a new pathogen. We tested the feasibility of a system based on antimicrobial synbodies. The system involves creating an array of 100 peptides that have been selected for broad capability to bind and/or kill viruses and bacteria. The peptides are pre-screened for low cell toxicity prior to large scale synthesis. Any pathogen is then assayed on the chip to find peptides that bind or kill it. Peptides are combined in pairs as synbodies and further screened for activity and toxicity. The lead synbody can be quickly produced in large scale, with completion of the entire process in one week.

Original languageEnglish (US)
Article number17610
JournalScientific Reports
Volume7
Issue number1
DOIs
StatePublished - Dec 1 2017

Fingerprint

Peptides
Pathogens
Toxicity
Viruses
Bacteria
Lead

ASJC Scopus subject areas

  • General

Cite this

A Simple Platform for the Rapid Development of Antimicrobials. / Johnston, Stephen; Domenyuk, Valeriy; Gupta, Nidhi; Batista, Milene Tavares; Lainson, John C.; Zhao, Zhan-Gong; Lusk, Joel F.; Loskutov, Andrey; Cichacz, Zbigniew; Stafford, Phillip; Legutki, Joseph Barten; Diehnelt, Chris.

In: Scientific Reports, Vol. 7, No. 1, 17610, 01.12.2017.

Research output: Contribution to journalArticle

Johnston, S, Domenyuk, V, Gupta, N, Batista, MT, Lainson, JC, Zhao, Z-G, Lusk, JF, Loskutov, A, Cichacz, Z, Stafford, P, Legutki, JB & Diehnelt, C 2017, 'A Simple Platform for the Rapid Development of Antimicrobials', Scientific Reports, vol. 7, no. 1, 17610. https://doi.org/10.1038/s41598-017-17941-7
Johnston, Stephen ; Domenyuk, Valeriy ; Gupta, Nidhi ; Batista, Milene Tavares ; Lainson, John C. ; Zhao, Zhan-Gong ; Lusk, Joel F. ; Loskutov, Andrey ; Cichacz, Zbigniew ; Stafford, Phillip ; Legutki, Joseph Barten ; Diehnelt, Chris. / A Simple Platform for the Rapid Development of Antimicrobials. In: Scientific Reports. 2017 ; Vol. 7, No. 1.
@article{5390daef06484531b3d3acdbd1a4de25,
title = "A Simple Platform for the Rapid Development of Antimicrobials",
abstract = "Recent infectious outbreaks highlight the need for platform technologies that can be quickly deployed to develop therapeutics needed to contain the outbreak. We present a simple concept for rapid development of new antimicrobials. The goal was to produce in as little as one week thousands of doses of an intervention for a new pathogen. We tested the feasibility of a system based on antimicrobial synbodies. The system involves creating an array of 100 peptides that have been selected for broad capability to bind and/or kill viruses and bacteria. The peptides are pre-screened for low cell toxicity prior to large scale synthesis. Any pathogen is then assayed on the chip to find peptides that bind or kill it. Peptides are combined in pairs as synbodies and further screened for activity and toxicity. The lead synbody can be quickly produced in large scale, with completion of the entire process in one week.",
author = "Stephen Johnston and Valeriy Domenyuk and Nidhi Gupta and Batista, {Milene Tavares} and Lainson, {John C.} and Zhan-Gong Zhao and Lusk, {Joel F.} and Andrey Loskutov and Zbigniew Cichacz and Phillip Stafford and Legutki, {Joseph Barten} and Chris Diehnelt",
year = "2017",
month = "12",
day = "1",
doi = "10.1038/s41598-017-17941-7",
language = "English (US)",
volume = "7",
journal = "Scientific Reports",
issn = "2045-2322",
publisher = "Nature Publishing Group",
number = "1",

}

TY - JOUR

T1 - A Simple Platform for the Rapid Development of Antimicrobials

AU - Johnston, Stephen

AU - Domenyuk, Valeriy

AU - Gupta, Nidhi

AU - Batista, Milene Tavares

AU - Lainson, John C.

AU - Zhao, Zhan-Gong

AU - Lusk, Joel F.

AU - Loskutov, Andrey

AU - Cichacz, Zbigniew

AU - Stafford, Phillip

AU - Legutki, Joseph Barten

AU - Diehnelt, Chris

PY - 2017/12/1

Y1 - 2017/12/1

N2 - Recent infectious outbreaks highlight the need for platform technologies that can be quickly deployed to develop therapeutics needed to contain the outbreak. We present a simple concept for rapid development of new antimicrobials. The goal was to produce in as little as one week thousands of doses of an intervention for a new pathogen. We tested the feasibility of a system based on antimicrobial synbodies. The system involves creating an array of 100 peptides that have been selected for broad capability to bind and/or kill viruses and bacteria. The peptides are pre-screened for low cell toxicity prior to large scale synthesis. Any pathogen is then assayed on the chip to find peptides that bind or kill it. Peptides are combined in pairs as synbodies and further screened for activity and toxicity. The lead synbody can be quickly produced in large scale, with completion of the entire process in one week.

AB - Recent infectious outbreaks highlight the need for platform technologies that can be quickly deployed to develop therapeutics needed to contain the outbreak. We present a simple concept for rapid development of new antimicrobials. The goal was to produce in as little as one week thousands of doses of an intervention for a new pathogen. We tested the feasibility of a system based on antimicrobial synbodies. The system involves creating an array of 100 peptides that have been selected for broad capability to bind and/or kill viruses and bacteria. The peptides are pre-screened for low cell toxicity prior to large scale synthesis. Any pathogen is then assayed on the chip to find peptides that bind or kill it. Peptides are combined in pairs as synbodies and further screened for activity and toxicity. The lead synbody can be quickly produced in large scale, with completion of the entire process in one week.

UR - http://www.scopus.com/inward/record.url?scp=85038230859&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85038230859&partnerID=8YFLogxK

U2 - 10.1038/s41598-017-17941-7

DO - 10.1038/s41598-017-17941-7

M3 - Article

VL - 7

JO - Scientific Reports

JF - Scientific Reports

SN - 2045-2322

IS - 1

M1 - 17610

ER -