A second site for herbicide action in Photosystem II

Robert Carpentier; E. Patrick Fuerst; Herbert Y. Nakatani; Charles J. Arntzen

doi:10.1016/0005-2728(85)90012-X

A second site for herbicide action in Photosystem II

Robert Carpentier, E. Patrick Fuerst, Herbert Y. Nakatani, Charles J. Arntzen

Arizona State University

Research output: Contribution to journal › Article › peer-review

42 Scopus citations

Abstract

The results in this report confirm the existence of a second site of herbicide inhibition on the oxidizing side of Photosystem II (PS II). The inhibitors which were examined in this study were initially found to inhibit the photoreduction of silicomolybdate with chloroplast thylakoids utilizing water as the electron donor. This indicated a site other than the well-characterized inhibition site on the reducing side of PS II at the 32 kDa polypeptide, Q_b apoprotein. Further studies were conducted utilizing Triton X-100 extracted PS II oxygen-evolving particles from spinach and atrazine-resistant and susceptible Amaranthus hybridis. Fluorescence data and the ability to overcome the inhibition of DCIP reduction by diphenylcarbazide both indicated that the oxidizing side of PS II was affected. ¹⁴C-atrazine binding studies are also presented.

Original language	English (US)
Pages (from-to)	293-299
Number of pages	7
Journal	BBA - Bioenergetics
Volume	808
Issue number	2
DOIs	https://doi.org/10.1016/0005-2728(85)90012-X
State	Published - Jul 17 1985

Keywords

(Pea, Spinach, Amaranthus chloroplasts)
Electron transport
Herbicide
Oxygen evolution
Photosystem II

ASJC Scopus subject areas

Biophysics
Biochemistry
Cell Biology

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10.1016/0005-2728(85)90012-X

Cite this

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title = "A second site for herbicide action in Photosystem II",

abstract = "The results in this report confirm the existence of a second site of herbicide inhibition on the oxidizing side of Photosystem II (PS II). The inhibitors which were examined in this study were initially found to inhibit the photoreduction of silicomolybdate with chloroplast thylakoids utilizing water as the electron donor. This indicated a site other than the well-characterized inhibition site on the reducing side of PS II at the 32 kDa polypeptide, Qb apoprotein. Further studies were conducted utilizing Triton X-100 extracted PS II oxygen-evolving particles from spinach and atrazine-resistant and susceptible Amaranthus hybridis. Fluorescence data and the ability to overcome the inhibition of DCIP reduction by diphenylcarbazide both indicated that the oxidizing side of PS II was affected. 14C-atrazine binding studies are also presented.",

keywords = "(Pea, Spinach, Amaranthus chloroplasts), Electron transport, Herbicide, Oxygen evolution, Photosystem II",

author = "Robert Carpentier and Fuerst, {E. Patrick} and Nakatani, {Herbert Y.} and Arntzen, {Charles J.}",

note = "Funding Information: This researchw as supportedin part by NSF grant No. 8023031a nd DOE contracNt o. AC02-76ER0-1338R. .C. is a recipiento f a. fellowship from the Natural Sciencesa nd EngineeringR e-searchC ouncilof Canada( NSERC).",

year = "1985",

month = jul,

day = "17",

doi = "10.1016/0005-2728(85)90012-X",

language = "English (US)",

volume = "808",

pages = "293--299",

journal = "BBA - Bioenergetics",

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T1 - A second site for herbicide action in Photosystem II

AU - Carpentier, Robert

AU - Fuerst, E. Patrick

AU - Nakatani, Herbert Y.

AU - Arntzen, Charles J.

N1 - Funding Information: This researchw as supportedin part by NSF grant No. 8023031a nd DOE contracNt o. AC02-76ER0-1338R. .C. is a recipiento f a. fellowship from the Natural Sciencesa nd EngineeringR e-searchC ouncilof Canada( NSERC).

PY - 1985/7/17

Y1 - 1985/7/17

N2 - The results in this report confirm the existence of a second site of herbicide inhibition on the oxidizing side of Photosystem II (PS II). The inhibitors which were examined in this study were initially found to inhibit the photoreduction of silicomolybdate with chloroplast thylakoids utilizing water as the electron donor. This indicated a site other than the well-characterized inhibition site on the reducing side of PS II at the 32 kDa polypeptide, Qb apoprotein. Further studies were conducted utilizing Triton X-100 extracted PS II oxygen-evolving particles from spinach and atrazine-resistant and susceptible Amaranthus hybridis. Fluorescence data and the ability to overcome the inhibition of DCIP reduction by diphenylcarbazide both indicated that the oxidizing side of PS II was affected. 14C-atrazine binding studies are also presented.

AB - The results in this report confirm the existence of a second site of herbicide inhibition on the oxidizing side of Photosystem II (PS II). The inhibitors which were examined in this study were initially found to inhibit the photoreduction of silicomolybdate with chloroplast thylakoids utilizing water as the electron donor. This indicated a site other than the well-characterized inhibition site on the reducing side of PS II at the 32 kDa polypeptide, Qb apoprotein. Further studies were conducted utilizing Triton X-100 extracted PS II oxygen-evolving particles from spinach and atrazine-resistant and susceptible Amaranthus hybridis. Fluorescence data and the ability to overcome the inhibition of DCIP reduction by diphenylcarbazide both indicated that the oxidizing side of PS II was affected. 14C-atrazine binding studies are also presented.

KW - (Pea, Spinach, Amaranthus chloroplasts)

KW - Electron transport

KW - Herbicide

KW - Oxygen evolution

KW - Photosystem II

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M3 - Article

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SN - 0005-2728

VL - 808

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JO - BBA - Bioenergetics

JF - BBA - Bioenergetics

IS - 2

ER -

A second site for herbicide action in Photosystem II

Abstract

Keywords

ASJC Scopus subject areas

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