A preliminary implementation of metabolic-based pH control to reduce CO2 usage in outdoor flat-panel photobioreactor cultivation of Nannochloropsis oceanica microalgae

Jun Wang, Theresa Rosov, Pierre Wensel, John McGowen, Wayne R. Curtis

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

A crucial challenge associated with high-density, commercial-scale, outdoor microalgal cultivation is maintaining pH stability without excessive use of CO2 buffering. This includes a media-dependent, intracellular metabolic proton imbalance leading to the alkalization or acidification of growth media that results when algae consume, respectively, nitrate or ammonium ions. Feeding these two nitrogen sources can theoretically achieve balanced proton metabolism as well as pH control that is economically more favorable as compared to CO2 buffering or acid/base addition. To accomplish this, a fed-batch nutrient feeding strategy must be adopted as a component of a model-based pH control system, particularly in the case of ammonium preference. This work represents a preliminary study of the challenges of implementing a nitrogen metabolism based open-loop pH control strategy in the challenging environment of an outdoor photobioreactor at the DOE-ATP3 testbed facility in Mesa, Arizona during the high solar insolation period of summer 2015. The approach was limited to twice daily fed-batch addition while accounting for ammonium-N preference in a background of nitrate-based algae growth media. Despite these limitations, growth achieved for a photobioreactor operated based on predicted metabolic nitrogen demand (PND) was comparable to 'CO2-on-demand' (CoD) for pH control. PND reduced CO2 usage to

Original languageEnglish (US)
Pages (from-to)288-295
Number of pages8
JournalAlgal Research
Volume18
DOIs
StatePublished - Sep 1 2016

Fingerprint

Nannochloropsis
microalgae
algae
protons
nitrogen
culture media
nitrates
alkalinization
ammonium compounds
feeding methods
nitrogen metabolism
acidification
solar radiation
metabolism
acids
summer
nutrients

Keywords

  • Algae cultivation
  • Alkalinity
  • Carbon yield
  • Nannochloropsis oceanic
  • Nitrogen assimilation
  • PH control
  • Photobioreactor
  • Stoichiometry

ASJC Scopus subject areas

  • Agronomy and Crop Science

Cite this

A preliminary implementation of metabolic-based pH control to reduce CO2 usage in outdoor flat-panel photobioreactor cultivation of Nannochloropsis oceanica microalgae. / Wang, Jun; Rosov, Theresa; Wensel, Pierre; McGowen, John; Curtis, Wayne R.

In: Algal Research, Vol. 18, 01.09.2016, p. 288-295.

Research output: Contribution to journalArticle

@article{7dd8fb2882454402a4978ebd41c80364,
title = "A preliminary implementation of metabolic-based pH control to reduce CO2 usage in outdoor flat-panel photobioreactor cultivation of Nannochloropsis oceanica microalgae",
abstract = "A crucial challenge associated with high-density, commercial-scale, outdoor microalgal cultivation is maintaining pH stability without excessive use of CO2 buffering. This includes a media-dependent, intracellular metabolic proton imbalance leading to the alkalization or acidification of growth media that results when algae consume, respectively, nitrate or ammonium ions. Feeding these two nitrogen sources can theoretically achieve balanced proton metabolism as well as pH control that is economically more favorable as compared to CO2 buffering or acid/base addition. To accomplish this, a fed-batch nutrient feeding strategy must be adopted as a component of a model-based pH control system, particularly in the case of ammonium preference. This work represents a preliminary study of the challenges of implementing a nitrogen metabolism based open-loop pH control strategy in the challenging environment of an outdoor photobioreactor at the DOE-ATP3 testbed facility in Mesa, Arizona during the high solar insolation period of summer 2015. The approach was limited to twice daily fed-batch addition while accounting for ammonium-N preference in a background of nitrate-based algae growth media. Despite these limitations, growth achieved for a photobioreactor operated based on predicted metabolic nitrogen demand (PND) was comparable to 'CO2-on-demand' (CoD) for pH control. PND reduced CO2 usage to",
keywords = "Algae cultivation, Alkalinity, Carbon yield, Nannochloropsis oceanic, Nitrogen assimilation, PH control, Photobioreactor, Stoichiometry",
author = "Jun Wang and Theresa Rosov and Pierre Wensel and John McGowen and Curtis, {Wayne R.}",
year = "2016",
month = "9",
day = "1",
doi = "10.1016/j.algal.2016.07.001",
language = "English (US)",
volume = "18",
pages = "288--295",
journal = "Algal Research",
issn = "2211-9264",
publisher = "Elsevier BV",

}

TY - JOUR

T1 - A preliminary implementation of metabolic-based pH control to reduce CO2 usage in outdoor flat-panel photobioreactor cultivation of Nannochloropsis oceanica microalgae

AU - Wang, Jun

AU - Rosov, Theresa

AU - Wensel, Pierre

AU - McGowen, John

AU - Curtis, Wayne R.

PY - 2016/9/1

Y1 - 2016/9/1

N2 - A crucial challenge associated with high-density, commercial-scale, outdoor microalgal cultivation is maintaining pH stability without excessive use of CO2 buffering. This includes a media-dependent, intracellular metabolic proton imbalance leading to the alkalization or acidification of growth media that results when algae consume, respectively, nitrate or ammonium ions. Feeding these two nitrogen sources can theoretically achieve balanced proton metabolism as well as pH control that is economically more favorable as compared to CO2 buffering or acid/base addition. To accomplish this, a fed-batch nutrient feeding strategy must be adopted as a component of a model-based pH control system, particularly in the case of ammonium preference. This work represents a preliminary study of the challenges of implementing a nitrogen metabolism based open-loop pH control strategy in the challenging environment of an outdoor photobioreactor at the DOE-ATP3 testbed facility in Mesa, Arizona during the high solar insolation period of summer 2015. The approach was limited to twice daily fed-batch addition while accounting for ammonium-N preference in a background of nitrate-based algae growth media. Despite these limitations, growth achieved for a photobioreactor operated based on predicted metabolic nitrogen demand (PND) was comparable to 'CO2-on-demand' (CoD) for pH control. PND reduced CO2 usage to

AB - A crucial challenge associated with high-density, commercial-scale, outdoor microalgal cultivation is maintaining pH stability without excessive use of CO2 buffering. This includes a media-dependent, intracellular metabolic proton imbalance leading to the alkalization or acidification of growth media that results when algae consume, respectively, nitrate or ammonium ions. Feeding these two nitrogen sources can theoretically achieve balanced proton metabolism as well as pH control that is economically more favorable as compared to CO2 buffering or acid/base addition. To accomplish this, a fed-batch nutrient feeding strategy must be adopted as a component of a model-based pH control system, particularly in the case of ammonium preference. This work represents a preliminary study of the challenges of implementing a nitrogen metabolism based open-loop pH control strategy in the challenging environment of an outdoor photobioreactor at the DOE-ATP3 testbed facility in Mesa, Arizona during the high solar insolation period of summer 2015. The approach was limited to twice daily fed-batch addition while accounting for ammonium-N preference in a background of nitrate-based algae growth media. Despite these limitations, growth achieved for a photobioreactor operated based on predicted metabolic nitrogen demand (PND) was comparable to 'CO2-on-demand' (CoD) for pH control. PND reduced CO2 usage to

KW - Algae cultivation

KW - Alkalinity

KW - Carbon yield

KW - Nannochloropsis oceanic

KW - Nitrogen assimilation

KW - PH control

KW - Photobioreactor

KW - Stoichiometry

UR - http://www.scopus.com/inward/record.url?scp=84977490449&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84977490449&partnerID=8YFLogxK

U2 - 10.1016/j.algal.2016.07.001

DO - 10.1016/j.algal.2016.07.001

M3 - Article

AN - SCOPUS:84977490449

VL - 18

SP - 288

EP - 295

JO - Algal Research

JF - Algal Research

SN - 2211-9264

ER -