A poxvirus-encoded pyrin domain protein interacts with ASC-1 to inhibit host inflammatory and apoptotic responses to infection

James B. Johnston, John W. Barrett, Steven H. Nazarian, Megan Goodwin, Dan Ricuttio, Gen Wang, Douglas McFadden

Research output: Contribution to journalArticle

162 Citations (Scopus)

Abstract

Proinflammatory caspases play an essential role in innate immune responses to infection by regulating the cleavage and activation of proinflammatory cytokines. Activation of these enzymes requires the assembly of an intracellular molecular platform, termed the inflammasome, which is comprised of members of the pyrin domain (PYD)-containing superfamily of apoptosis and inflammation-regulatory proteins. We report here the identification and characterization of a poxvirus-encoded PYD-containing protein that interacts with the ASC-1 component of the inflammasome and inhibits caspase-1 activation and the processing of IL-1β and IL-18 induced by diverse stimuli. Knockout viruses that do not express this protein are unable to productively infect monocytes and lymphocytes due to an abortive phenotype and are markedly attenuated in susceptible hosts due to decreased virus dissemination and enhanced inflammatory responses at sites of infection. Thus, modulation of inflammasome function constitutes an important immunomodulatory strategy employed by poxviruses to circumvent host antiviral responses.

Original languageEnglish (US)
Pages (from-to)587-598
Number of pages12
JournalImmunity
Volume23
Issue number6
DOIs
StatePublished - Dec 1 2005
Externally publishedYes

Fingerprint

Inflammasomes
Poxviridae
Infection
Viruses
Caspase 1
Apoptosis Regulatory Proteins
Proteins
Interleukin-18
Enzyme Activation
Caspases
Interleukin-1
Innate Immunity
Antiviral Agents
Monocytes
Lymphocytes
Cytokines
Inflammation
Phenotype
Pyrin Domain

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Infectious Diseases

Cite this

A poxvirus-encoded pyrin domain protein interacts with ASC-1 to inhibit host inflammatory and apoptotic responses to infection. / Johnston, James B.; Barrett, John W.; Nazarian, Steven H.; Goodwin, Megan; Ricuttio, Dan; Wang, Gen; McFadden, Douglas.

In: Immunity, Vol. 23, No. 6, 01.12.2005, p. 587-598.

Research output: Contribution to journalArticle

Johnston, James B. ; Barrett, John W. ; Nazarian, Steven H. ; Goodwin, Megan ; Ricuttio, Dan ; Wang, Gen ; McFadden, Douglas. / A poxvirus-encoded pyrin domain protein interacts with ASC-1 to inhibit host inflammatory and apoptotic responses to infection. In: Immunity. 2005 ; Vol. 23, No. 6. pp. 587-598.
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