A Novel Approach to High-Quality Postmortem Tissue Procurement: The GTEx Project

Latarsha J. Carithers; Kristin Ardlie; Mary Barcus; Philip A. Branton; Angela Britton; Stephen A. Buia; Carolyn C. Compton; David S. Deluca; Joanne Peter-Demchok; Ellen T. Gelfand; Ping Guan; Greg E. Korzeniewski; Nicole C. Lockhart; Chana A. Rabiner; Abhi K. Rao; Karna L. Robinson; Nancy V. Roche; Sherilyn J. Sawyer; Ayellet V. Segrè; Charles E. Shive; Anna M. Smith; Leslie H. Sobin; Anita H. Undale; Kimberly M. Valentino; Jim Vaught; Taylor R. Young; Helen M. Moore; Laura Barker; Margaret Basile; Alexis Battle; Joy Boyer; Debra Bradbury; Jason P. Bridge; Amanda Brown; Robin Burges; Christopher Choi; Deborah Colantuoni; Nancy Cox; Emmanouil T. Dermitzakis; Leslie K. Derr; Michael J. Dinsmore; Kenyon Erickson; Johnelle Fleming; Timothée Flutre; Barbara A. Foster; Eric R. Gamazon; Gad Getz; Bryan M. Gillard; Roderic Guigó; Kenneth W. Hambright; Pushpa Hariharan; Rick Hasz; Hae K. Im; Scott Jewell; Ellen Karasik; Manolis Kellis; Pouya Kheradpour; Susan Koester; Daphne Koller; Anuar Konkashbaev; Tuuli Lappalainen; Roger Little; Jun Liu; Edmund Lo; John T. Lonsdale; Chunrong Lu; Daniel G. MacArthur; Harold Magazine; Julian B. Maller; Yvonne Marcus; Deborah C. Mash; Mark I. McCarthy; Jeffrey McLean; Bernadette Mestichelli; Mark Miklos; Jean Monlong; Magboeba Mosavel; Michael T. Moser; Sara Mostafavi; Dan L. Nicolae; Jonathan Pritchard; Liqun Qi; Kimberly Ramsey; Manuel A. Rivas; Barnaby E. Robles; Daniel C. Rohrer; Mike Salvatore; Michael Sammeth; John Seleski; Saboor Shad; Laura A. Siminoff; Matthew Stephens; Jeff Struewing; Timothy Sullivan; Susan Sullivan; John Syron; David Tabor; Mehran Taherian; Jorge Tejada; Gary F. Temple; Jeffrey A. Thomas; Alexander W. Thomson; Denee Tidwell; Heather M. Traino; Zhidong Tu; Dana R. Valley; Simona Volpi; Gary D. Walters; Lucas D. Ward; Xiaoquan Wen; Wendy Winckler; Shenpei Wu; Jun Zhu; Assya Abdallah; Anjene Addington; James M. Anderson; Patrick K. Bender; Mark Cosentino; Norma Diaz-Mayoral; Theresa Engel; Fernando Garci; Allen Green; Tiffanie Hammond; Katherine Jaffe; Judy Keen; Mary Kennedy; Peter Kigonya; Brent Lander; Sreenath Nampally; Cathy Ny; James Robb; Vikram Santhanum; Nataliya Sharopova; Shilpi Singh; Conrado Soria; Anne Sturcke; Surendra Sukari; Elizabeth J. Thomson; Magda Tomaszewski; Casandra Trowbridge; Ferdinand Udoye; David Vanscoy; Negin Vatanian; Elizabeth L. Wilder; Penelope Williams

doi:10.1089/bio.2015.0032

A Novel Approach to High-Quality Postmortem Tissue Procurement: The GTEx Project

Latarsha J. Carithers, Kristin Ardlie, Mary Barcus, Philip A. Branton, Angela Britton, Stephen A. Buia, Carolyn C. Compton, David S. Deluca, Joanne Peter-Demchok, Ellen T. Gelfand, Ping Guan, Greg E. Korzeniewski, Nicole C. Lockhart, Chana A. Rabiner, Abhi K. Rao, Karna L. Robinson, Nancy V. Roche, Sherilyn J. Sawyer, Ayellet V. Segrè, Charles E. ShiveAnna M. Smith, Leslie H. Sobin, Anita H. Undale, Kimberly M. Valentino, Jim Vaught, Taylor R. Young, Helen M. Moore, Laura Barker, Margaret Basile, Alexis Battle, Joy Boyer, Debra Bradbury, Jason P. Bridge, Amanda Brown, Robin Burges, Christopher Choi, Deborah Colantuoni, Nancy Cox, Emmanouil T. Dermitzakis, Leslie K. Derr, Michael J. Dinsmore, Kenyon Erickson, Johnelle Fleming, Timothée Flutre, Barbara A. Foster, Eric R. Gamazon, Gad Getz, Bryan M. Gillard, Roderic Guigó, Kenneth W. Hambright, Pushpa Hariharan, Rick Hasz, Hae K. Im, Scott Jewell, Ellen Karasik, Manolis Kellis, Pouya Kheradpour, Susan Koester, Daphne Koller, Anuar Konkashbaev, Tuuli Lappalainen, Roger Little, Jun Liu, Edmund Lo, John T. Lonsdale, Chunrong Lu, Daniel G. MacArthur, Harold Magazine, Julian B. Maller, Yvonne Marcus, Deborah C. Mash, Mark I. McCarthy, Jeffrey McLean, Bernadette Mestichelli, Mark Miklos, Jean Monlong, Magboeba Mosavel, Michael T. Moser, Sara Mostafavi, Dan L. Nicolae, Jonathan Pritchard, Liqun Qi, Kimberly Ramsey, Manuel A. Rivas, Barnaby E. Robles, Daniel C. Rohrer, Mike Salvatore, Michael Sammeth, John Seleski, Saboor Shad, Laura A. Siminoff, Matthew Stephens, Jeff Struewing, Timothy Sullivan, Susan Sullivan, John Syron, David Tabor, Mehran Taherian, Jorge Tejada, Gary F. Temple, Jeffrey A. Thomas, Alexander W. Thomson, Denee Tidwell, Heather M. Traino, Zhidong Tu, Dana R. Valley, Simona Volpi, Gary D. Walters, Lucas D. Ward, Xiaoquan Wen, Wendy Winckler, Shenpei Wu, Jun Zhu, Assya Abdallah, Anjene Addington, James M. Anderson, Patrick K. Bender, Mark Cosentino, Norma Diaz-Mayoral, Theresa Engel, Fernando Garci, Allen Green, Tiffanie Hammond, Katherine Jaffe, Judy Keen, Mary Kennedy, Peter Kigonya, Brent Lander, Sreenath Nampally, Cathy Ny, James Robb, Vikram Santhanum, Nataliya Sharopova, Shilpi Singh, Conrado Soria, Anne Sturcke, Surendra Sukari, Elizabeth J. Thomson, Magda Tomaszewski, Casandra Trowbridge, Ferdinand Udoye, David Vanscoy, Negin Vatanian, Elizabeth L. Wilder, Penelope Williams

Research output: Contribution to journal › Article › peer-review

514 Scopus citations

Abstract

The Genotype-Tissue Expression (GTEx) project, sponsored by the NIH Common Fund, was established to study the correlation between human genetic variation and tissue-specific gene expression in non-diseased individuals. A significant challenge was the collection of high-quality biospecimens for extensive genomic analyses. Here we describe how a successful infrastructure for biospecimen procurement was developed and implemented by multiple research partners to support the prospective collection, annotation, and distribution of blood, tissues, and cell lines for the GTEx project. Other research projects can follow this model and form beneficial partnerships with rapid autopsy and organ procurement organizations to collect high quality biospecimens and associated clinical data for genomic studies. Biospecimens, clinical and genomic data, and Standard Operating Procedures guiding biospecimen collection for the GTEx project are available to the research community.

Original language	English (US)
Pages (from-to)	311-317
Number of pages	7
Journal	Biopreservation and Biobanking
Volume	13
Issue number	5
DOIs	https://doi.org/10.1089/bio.2015.0032
State	Published - Oct 2015
Externally published	Yes

ASJC Scopus subject areas

Medicine (miscellaneous)
General Biochemistry, Genetics and Molecular Biology
Cell Biology

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10.1089/bio.2015.0032

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Carithers, L. J., Ardlie, K., Barcus, M., Branton, P. A., Britton, A., Buia, S. A., Compton, C. C., Deluca, D. S., Peter-Demchok, J., Gelfand, E. T., Guan, P., Korzeniewski, G. E., Lockhart, N. C., Rabiner, C. A., Rao, A. K., Robinson, K. L., Roche, N. V., Sawyer, S. J., Segrè, A. V., ... Williams, P. (2015). A Novel Approach to High-Quality Postmortem Tissue Procurement: The GTEx Project. Biopreservation and Biobanking, 13(5), 311-317. https://doi.org/10.1089/bio.2015.0032

Carithers, LJ, Ardlie, K, Barcus, M, Branton, PA, Britton, A, Buia, SA, Compton, CC, Deluca, DS, Peter-Demchok, J, Gelfand, ET, Guan, P, Korzeniewski, GE, Lockhart, NC, Rabiner, CA, Rao, AK, Robinson, KL, Roche, NV, Sawyer, SJ, Segrè, AV, Shive, CE, Smith, AM, Sobin, LH, Undale, AH, Valentino, KM, Vaught, J, Young, TR, Moore, HM, Barker, L, Basile, M, Battle, A, Boyer, J, Bradbury, D, Bridge, JP, Brown, A, Burges, R, Choi, C, Colantuoni, D, Cox, N, Dermitzakis, ET, Derr, LK, Dinsmore, MJ, Erickson, K, Fleming, J, Flutre, T, Foster, BA, Gamazon, ER, Getz, G, Gillard, BM, Guigó, R, Hambright, KW, Hariharan, P, Hasz, R, Im, HK, Jewell, S, Karasik, E, Kellis, M, Kheradpour, P, Koester, S, Koller, D, Konkashbaev, A, Lappalainen, T, Little, R, Liu, J, Lo, E, Lonsdale, JT, Lu, C, MacArthur, DG, Magazine, H, Maller, JB, Marcus, Y, Mash, DC, McCarthy, MI, McLean, J, Mestichelli, B, Miklos, M, Monlong, J, Mosavel, M, Moser, MT, Mostafavi, S, Nicolae, DL, Pritchard, J, Qi, L, Ramsey, K, Rivas, MA, Robles, BE, Rohrer, DC, Salvatore, M, Sammeth, M, Seleski, J, Shad, S, Siminoff, LA, Stephens, M, Struewing, J, Sullivan, T, Sullivan, S, Syron, J, Tabor, D, Taherian, M, Tejada, J, Temple, GF, Thomas, JA, Thomson, AW, Tidwell, D, Traino, HM, Tu, Z, Valley, DR, Volpi, S, Walters, GD, Ward, LD, Wen, X, Winckler, W, Wu, S, Zhu, J, Abdallah, A, Addington, A, Anderson, JM, Bender, PK, Cosentino, M, Diaz-Mayoral, N, Engel, T, Garci, F, Green, A, Hammond, T, Jaffe, K, Keen, J, Kennedy, M, Kigonya, P, Lander, B, Nampally, S, Ny, C, Robb, J, Santhanum, V, Sharopova, N, Singh, S, Soria, C, Sturcke, A, Sukari, S, Thomson, EJ, Tomaszewski, M, Trowbridge, C, Udoye, F, Vanscoy, D, Vatanian, N, Wilder, EL & Williams, P 2015, 'A Novel Approach to High-Quality Postmortem Tissue Procurement: The GTEx Project', Biopreservation and Biobanking, vol. 13, no. 5, pp. 311-317. https://doi.org/10.1089/bio.2015.0032

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abstract = "The Genotype-Tissue Expression (GTEx) project, sponsored by the NIH Common Fund, was established to study the correlation between human genetic variation and tissue-specific gene expression in non-diseased individuals. A significant challenge was the collection of high-quality biospecimens for extensive genomic analyses. Here we describe how a successful infrastructure for biospecimen procurement was developed and implemented by multiple research partners to support the prospective collection, annotation, and distribution of blood, tissues, and cell lines for the GTEx project. Other research projects can follow this model and form beneficial partnerships with rapid autopsy and organ procurement organizations to collect high quality biospecimens and associated clinical data for genomic studies. Biospecimens, clinical and genomic data, and Standard Operating Procedures guiding biospecimen collection for the GTEx project are available to the research community.",

author = "Carithers, {Latarsha J.} and Kristin Ardlie and Mary Barcus and Branton, {Philip A.} and Angela Britton and Buia, {Stephen A.} and Compton, {Carolyn C.} and Deluca, {David S.} and Joanne Peter-Demchok and Gelfand, {Ellen T.} and Ping Guan and Korzeniewski, {Greg E.} and Lockhart, {Nicole C.} and Rabiner, {Chana A.} and Rao, {Abhi K.} and Robinson, {Karna L.} and Roche, {Nancy V.} and Sawyer, {Sherilyn J.} and Segr{\`e}, {Ayellet V.} and Shive, {Charles E.} and Smith, {Anna M.} and Sobin, {Leslie H.} and Undale, {Anita H.} and Valentino, {Kimberly M.} and Jim Vaught and Young, {Taylor R.} and Moore, {Helen M.} and Laura Barker and Margaret Basile and Alexis Battle and Joy Boyer and Debra Bradbury and Bridge, {Jason P.} and Amanda Brown and Robin Burges and Christopher Choi and Deborah Colantuoni and Nancy Cox and Dermitzakis, {Emmanouil T.} and Derr, {Leslie K.} and Dinsmore, {Michael J.} and Kenyon Erickson and Johnelle Fleming and Timoth{\'e}e Flutre and Foster, {Barbara A.} and Gamazon, {Eric R.} and Gad Getz and Gillard, {Bryan M.} and Roderic Guig{\'o} and Hambright, {Kenneth W.} and Pushpa Hariharan and Rick Hasz and Im, {Hae K.} and Scott Jewell and Ellen Karasik and Manolis Kellis and Pouya Kheradpour and Susan Koester and Daphne Koller and Anuar Konkashbaev and Tuuli Lappalainen and Roger Little and Jun Liu and Edmund Lo and Lonsdale, {John T.} and Chunrong Lu and MacArthur, {Daniel G.} and Harold Magazine and Maller, {Julian B.} and Yvonne Marcus and Mash, {Deborah C.} and McCarthy, {Mark I.} and Jeffrey McLean and Bernadette Mestichelli and Mark Miklos and Jean Monlong and Magboeba Mosavel and Moser, {Michael T.} and Sara Mostafavi and Nicolae, {Dan L.} and Jonathan Pritchard and Liqun Qi and Kimberly Ramsey and Rivas, {Manuel A.} and Robles, {Barnaby E.} and Rohrer, {Daniel C.} and Mike Salvatore and Michael Sammeth and John Seleski and Saboor Shad and Siminoff, {Laura A.} and Matthew Stephens and Jeff Struewing and Timothy Sullivan and Susan Sullivan and John Syron and David Tabor and Mehran Taherian and Jorge Tejada and Temple, {Gary F.} and Thomas, {Jeffrey A.} and Thomson, {Alexander W.} and Denee Tidwell and Traino, {Heather M.} and Zhidong Tu and Valley, {Dana R.} and Simona Volpi and Walters, {Gary D.} and Ward, {Lucas D.} and Xiaoquan Wen and Wendy Winckler and Shenpei Wu and Jun Zhu and Assya Abdallah and Anjene Addington and Anderson, {James M.} and Bender, {Patrick K.} and Mark Cosentino and Norma Diaz-Mayoral and Theresa Engel and Fernando Garci and Allen Green and Tiffanie Hammond and Katherine Jaffe and Judy Keen and Mary Kennedy and Peter Kigonya and Brent Lander and Sreenath Nampally and Cathy Ny and James Robb and Vikram Santhanum and Nataliya Sharopova and Shilpi Singh and Conrado Soria and Anne Sturcke and Surendra Sukari and Thomson, {Elizabeth J.} and Magda Tomaszewski and Casandra Trowbridge and Ferdinand Udoye and David Vanscoy and Negin Vatanian and Wilder, {Elizabeth L.} and Penelope Williams",

year = "2015",

month = oct,

doi = "10.1089/bio.2015.0032",

language = "English (US)",

volume = "13",

pages = "311--317",

journal = "Biopreservation and Biobanking",

issn = "1947-5535",

publisher = "Mary Ann Liebert Inc.",

number = "5",

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TY - JOUR

T1 - A Novel Approach to High-Quality Postmortem Tissue Procurement

T2 - The GTEx Project

AU - Carithers, Latarsha J.

AU - Ardlie, Kristin

AU - Barcus, Mary

AU - Branton, Philip A.

AU - Britton, Angela

AU - Buia, Stephen A.

AU - Compton, Carolyn C.

AU - Deluca, David S.

AU - Peter-Demchok, Joanne

AU - Gelfand, Ellen T.

AU - Guan, Ping

AU - Korzeniewski, Greg E.

AU - Lockhart, Nicole C.

AU - Rabiner, Chana A.

AU - Rao, Abhi K.

AU - Robinson, Karna L.

AU - Roche, Nancy V.

AU - Sawyer, Sherilyn J.

AU - Segrè, Ayellet V.

AU - Shive, Charles E.

AU - Smith, Anna M.

AU - Sobin, Leslie H.

AU - Undale, Anita H.

AU - Valentino, Kimberly M.

AU - Vaught, Jim

AU - Young, Taylor R.

AU - Moore, Helen M.

AU - Barker, Laura

AU - Basile, Margaret

AU - Battle, Alexis

AU - Boyer, Joy

AU - Bradbury, Debra

AU - Bridge, Jason P.

AU - Brown, Amanda

AU - Burges, Robin

AU - Choi, Christopher

AU - Colantuoni, Deborah

AU - Cox, Nancy

AU - Dermitzakis, Emmanouil T.

AU - Derr, Leslie K.

AU - Dinsmore, Michael J.

AU - Erickson, Kenyon

AU - Fleming, Johnelle

AU - Flutre, Timothée

AU - Foster, Barbara A.

AU - Gamazon, Eric R.

AU - Getz, Gad

AU - Gillard, Bryan M.

AU - Guigó, Roderic

AU - Hambright, Kenneth W.

AU - Hariharan, Pushpa

AU - Hasz, Rick

AU - Im, Hae K.

AU - Jewell, Scott

AU - Karasik, Ellen

AU - Kellis, Manolis

AU - Kheradpour, Pouya

AU - Koester, Susan

AU - Koller, Daphne

AU - Konkashbaev, Anuar

AU - Lappalainen, Tuuli

AU - Little, Roger

AU - Liu, Jun

AU - Lo, Edmund

AU - Lonsdale, John T.

AU - Lu, Chunrong

AU - MacArthur, Daniel G.

AU - Magazine, Harold

AU - Maller, Julian B.

AU - Marcus, Yvonne

AU - Mash, Deborah C.

AU - McCarthy, Mark I.

AU - McLean, Jeffrey

AU - Mestichelli, Bernadette

AU - Miklos, Mark

AU - Monlong, Jean

AU - Mosavel, Magboeba

AU - Moser, Michael T.

AU - Mostafavi, Sara

AU - Nicolae, Dan L.

AU - Pritchard, Jonathan

AU - Qi, Liqun

AU - Ramsey, Kimberly

AU - Rivas, Manuel A.

AU - Robles, Barnaby E.

AU - Rohrer, Daniel C.

AU - Salvatore, Mike

AU - Sammeth, Michael

AU - Seleski, John

AU - Shad, Saboor

AU - Siminoff, Laura A.

AU - Stephens, Matthew

AU - Struewing, Jeff

AU - Sullivan, Timothy

AU - Sullivan, Susan

AU - Syron, John

AU - Tabor, David

AU - Taherian, Mehran

AU - Tejada, Jorge

AU - Temple, Gary F.

AU - Thomas, Jeffrey A.

AU - Thomson, Alexander W.

AU - Tidwell, Denee

AU - Traino, Heather M.

AU - Tu, Zhidong

AU - Valley, Dana R.

AU - Volpi, Simona

AU - Walters, Gary D.

AU - Ward, Lucas D.

AU - Wen, Xiaoquan

AU - Winckler, Wendy

AU - Wu, Shenpei

AU - Zhu, Jun

AU - Abdallah, Assya

AU - Addington, Anjene

AU - Anderson, James M.

AU - Bender, Patrick K.

AU - Cosentino, Mark

AU - Diaz-Mayoral, Norma

AU - Engel, Theresa

AU - Garci, Fernando

AU - Green, Allen

AU - Hammond, Tiffanie

AU - Jaffe, Katherine

AU - Keen, Judy

AU - Kennedy, Mary

AU - Kigonya, Peter

AU - Lander, Brent

AU - Nampally, Sreenath

AU - Ny, Cathy

AU - Robb, James

AU - Santhanum, Vikram

AU - Sharopova, Nataliya

AU - Singh, Shilpi

AU - Soria, Conrado

AU - Sturcke, Anne

AU - Sukari, Surendra

AU - Thomson, Elizabeth J.

AU - Tomaszewski, Magda

AU - Trowbridge, Casandra

AU - Udoye, Ferdinand

AU - Vanscoy, David

AU - Vatanian, Negin

AU - Wilder, Elizabeth L.

AU - Williams, Penelope

PY - 2015/10

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AB - The Genotype-Tissue Expression (GTEx) project, sponsored by the NIH Common Fund, was established to study the correlation between human genetic variation and tissue-specific gene expression in non-diseased individuals. A significant challenge was the collection of high-quality biospecimens for extensive genomic analyses. Here we describe how a successful infrastructure for biospecimen procurement was developed and implemented by multiple research partners to support the prospective collection, annotation, and distribution of blood, tissues, and cell lines for the GTEx project. Other research projects can follow this model and form beneficial partnerships with rapid autopsy and organ procurement organizations to collect high quality biospecimens and associated clinical data for genomic studies. Biospecimens, clinical and genomic data, and Standard Operating Procedures guiding biospecimen collection for the GTEx project are available to the research community.

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U2 - 10.1089/bio.2015.0032

DO - 10.1089/bio.2015.0032

M3 - Article

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AN - SCOPUS:84945292900

SN - 1947-5535

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EP - 317

JO - Biopreservation and Biobanking

JF - Biopreservation and Biobanking

IS - 5

ER -

A Novel Approach to High-Quality Postmortem Tissue Procurement: The GTEx Project

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