A new strategy for the synthesis of bisaminoacylated tRNAs

Ryan C. Nangreave; Larisa Dedkova; Shengxi Chen; Sidney Hecht

doi:10.1021/ol201993c

A new strategy for the synthesis of bisaminoacylated tRNAs

Ryan C. Nangreave, Larisa Dedkova, Shengxi Chen, Sidney Hecht

Research output: Contribution to journal › Article › peer-review

4 Scopus citations

Abstract

Tandemly activated tRNAs participate effectively in protein synthesis and exhibit superior chemical and biochemical stability compared to the more commonly used singly aminoacylated tRNAs. While several bisaminoacylated tRNAs have been prepared via the T4 RNA ligase-mediated condensation of bisaminoacylated pdCpAs and abbreviated tRNA transcripts (tRNA-C _OH), the bisaminoacylated pdCpAs are difficult to prepare when using bulky amino acids. Described herein is a new strategy for preparing bisaminoacylated tRNAs, applicable even for bulky amino acids.

Original language	English (US)
Pages (from-to)	4906-4909
Number of pages	4
Journal	Organic Letters
Volume	13
Issue number	18
DOIs	https://doi.org/10.1021/ol201993c
State	Published - Sep 16 2011

ASJC Scopus subject areas

Biochemistry
Physical and Theoretical Chemistry
Organic Chemistry

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10.1021/ol201993c

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abstract = "Tandemly activated tRNAs participate effectively in protein synthesis and exhibit superior chemical and biochemical stability compared to the more commonly used singly aminoacylated tRNAs. While several bisaminoacylated tRNAs have been prepared via the T4 RNA ligase-mediated condensation of bisaminoacylated pdCpAs and abbreviated tRNA transcripts (tRNA-C OH), the bisaminoacylated pdCpAs are difficult to prepare when using bulky amino acids. Described herein is a new strategy for preparing bisaminoacylated tRNAs, applicable even for bulky amino acids.",

author = "Nangreave, {Ryan C.} and Larisa Dedkova and Shengxi Chen and Sidney Hecht",

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AU - Nangreave, Ryan C.

AU - Dedkova, Larisa

AU - Chen, Shengxi

AU - Hecht, Sidney

PY - 2011/9/16

Y1 - 2011/9/16

N2 - Tandemly activated tRNAs participate effectively in protein synthesis and exhibit superior chemical and biochemical stability compared to the more commonly used singly aminoacylated tRNAs. While several bisaminoacylated tRNAs have been prepared via the T4 RNA ligase-mediated condensation of bisaminoacylated pdCpAs and abbreviated tRNA transcripts (tRNA-C OH), the bisaminoacylated pdCpAs are difficult to prepare when using bulky amino acids. Described herein is a new strategy for preparing bisaminoacylated tRNAs, applicable even for bulky amino acids.

AB - Tandemly activated tRNAs participate effectively in protein synthesis and exhibit superior chemical and biochemical stability compared to the more commonly used singly aminoacylated tRNAs. While several bisaminoacylated tRNAs have been prepared via the T4 RNA ligase-mediated condensation of bisaminoacylated pdCpAs and abbreviated tRNA transcripts (tRNA-C OH), the bisaminoacylated pdCpAs are difficult to prepare when using bulky amino acids. Described herein is a new strategy for preparing bisaminoacylated tRNAs, applicable even for bulky amino acids.

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A new strategy for the synthesis of bisaminoacylated tRNAs

Abstract

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