A Mn2+-dependent ribozyme

V. Dange, Reuel B. Van Atta, Sidney M. Hecht

Research output: Contribution to journalArticle

46 Scopus citations

Abstract

An RNA hairpin identical in sequence with the one formed during autocyclization of the 414-nucleotide Tetrahymena intervening sequence undergoes strand scission at a specific site in the presence of Mn2+. In addition to representing one of the smallest and simplest ribozymes possible, strand scission occurs readily under physiological conditions, is unaffected by the presence of Mg2+, and displays salt, pH, and temperature optima of potential use in exploiting Mn2+ as a regulatory switch in intact cells. The chemistry of strand scission of the RNA hairpin is described, as is the Mn2+-dependent solvolysis of a 231-nucleotide RNA transcript containing this structural motif.

Original languageEnglish (US)
Pages (from-to)585-588
Number of pages4
JournalScience
Volume248
Issue number4955
DOIs
StatePublished - Jan 1 1990
Externally publishedYes

ASJC Scopus subject areas

  • General

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    Dange, V., Van Atta, R. B., & Hecht, S. M. (1990). A Mn2+-dependent ribozyme. Science, 248(4955), 585-588. https://doi.org/10.1126/science.2185542