A Mn2+-dependent ribozyme

V. Dange, Reuel B. Van Atta, Sidney Hecht

Research output: Contribution to journalArticle

46 Citations (Scopus)

Abstract

An RNA hairpin identical in sequence with the one formed during autocyclization of the 414-nucleotide Tetrahymena intervening sequence undergoes strand scission at a specific site in the presence of Mn2+. In addition to representing one of the smallest and simplest ribozymes possible, strand scission occurs readily under physiological conditions, is unaffected by the presence of Mg2+, and displays salt, pH, and temperature optima of potential use in exploiting Mn2+ as a regulatory switch in intact cells. The chemistry of strand scission of the RNA hairpin is described, as is the Mn2+-dependent solvolysis of a 231-nucleotide RNA transcript containing this structural motif.

Original languageEnglish (US)
Pages (from-to)585-588
Number of pages4
JournalScience
Volume248
Issue number4955
StatePublished - May 4 1990
Externally publishedYes

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Catalytic RNA
RNA
Nucleotides
Tetrahymena
Introns
Salts
Temperature

ASJC Scopus subject areas

  • General

Cite this

Dange, V., Van Atta, R. B., & Hecht, S. (1990). A Mn2+-dependent ribozyme. Science, 248(4955), 585-588.

A Mn2+-dependent ribozyme. / Dange, V.; Van Atta, Reuel B.; Hecht, Sidney.

In: Science, Vol. 248, No. 4955, 04.05.1990, p. 585-588.

Research output: Contribution to journalArticle

Dange, V, Van Atta, RB & Hecht, S 1990, 'A Mn2+-dependent ribozyme', Science, vol. 248, no. 4955, pp. 585-588.
Dange V, Van Atta RB, Hecht S. A Mn2+-dependent ribozyme. Science. 1990 May 4;248(4955):585-588.
Dange, V. ; Van Atta, Reuel B. ; Hecht, Sidney. / A Mn2+-dependent ribozyme. In: Science. 1990 ; Vol. 248, No. 4955. pp. 585-588.
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