TY - JOUR
T1 - A fluorescent colorimetric pH sensor and the influences of matrices on sensing performances
AU - Tian, Yanqing
AU - Fuller, Emily
AU - Klug, Summer
AU - Lee, Fred
AU - Su, Fengyu
AU - Zhang, Liqiang
AU - Chao, Shih-Hui
AU - Meldrum, Deirdre
N1 - Funding Information:
This work was supported by the NIH National Human Genome Research Institute, Centers of Excellence in Genomic Science , grant number 5 P50 HG002360 , and NIH Common Fund LINCS program , grant number 5 U01 CA164250 , Professor Deirdre Meldrum, PI.
PY - 2013
Y1 - 2013
N2 - A fluorescent colorimetric pH sensor was developed by a polymerization of a monomeric fluorescein based green emitter (SM1) with a monomeric 2-dicyanomethylene-3-cyano-4,5,5-trimethyl-2,5-dihydrofuran derived red emitter (SM2) in poly(2-hydroxyethyl methacrylate)-co-polyacrylamide (PHEMA-co-PAM) matrices. Polymerized SM1 (PSM1) in the polymer matrices showed bright emissions at basic conditions and weak emissions at acidic conditions. Polymerized SM2 (PSM2) in the polymer matrices exhibited a vastly different response when compared to PSM1. The emissions of PSM2 are stronger under acidic conditions than those under basic conditions. When SM1 and SM2 were polymerized in the same polymer matrix, a dual emission sensor acting as a ratiometric pH sensor (PSM1,2) was successfully developed. Because the PSM1 and PSM2 exhibited different pH responses and separated emission windows, the changes in the emission colors were clearly observed in their dual color sensor of PSM1,2, which changed emission colors dramatically from green at pH 7 to red at pH 4, which was detected visually and/or by using a color camera under an excitation of 488 nm. In addition to the development of the dual color ratiometric pH sensor, we also studied the effects of different matrix compositions, crosslinkers, and charges on the reporting capabilities of the sensors (sensitivity and pKa).
AB - A fluorescent colorimetric pH sensor was developed by a polymerization of a monomeric fluorescein based green emitter (SM1) with a monomeric 2-dicyanomethylene-3-cyano-4,5,5-trimethyl-2,5-dihydrofuran derived red emitter (SM2) in poly(2-hydroxyethyl methacrylate)-co-polyacrylamide (PHEMA-co-PAM) matrices. Polymerized SM1 (PSM1) in the polymer matrices showed bright emissions at basic conditions and weak emissions at acidic conditions. Polymerized SM2 (PSM2) in the polymer matrices exhibited a vastly different response when compared to PSM1. The emissions of PSM2 are stronger under acidic conditions than those under basic conditions. When SM1 and SM2 were polymerized in the same polymer matrix, a dual emission sensor acting as a ratiometric pH sensor (PSM1,2) was successfully developed. Because the PSM1 and PSM2 exhibited different pH responses and separated emission windows, the changes in the emission colors were clearly observed in their dual color sensor of PSM1,2, which changed emission colors dramatically from green at pH 7 to red at pH 4, which was detected visually and/or by using a color camera under an excitation of 488 nm. In addition to the development of the dual color ratiometric pH sensor, we also studied the effects of different matrix compositions, crosslinkers, and charges on the reporting capabilities of the sensors (sensitivity and pKa).
KW - Charge
KW - Colorimetric sensors
KW - Crosslinkers
KW - Matrix
KW - pH sensors
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U2 - 10.1016/j.snb.2013.06.098
DO - 10.1016/j.snb.2013.06.098
M3 - Article
AN - SCOPUS:84881306520
SN - 0925-4005
VL - 188
SP - 1
EP - 10
JO - Sensors and Actuators, B: Chemical
JF - Sensors and Actuators, B: Chemical
ER -