A dual sensor for real-time monitoring of glucose and oxygen

Liqiang Zhang, Fengyu Su, Sean Buizer, Hongguang Lu, Weimin Gao, Yanqing Tian, Deirdre Meldrum

Research output: Contribution to journalArticle

21 Scopus citations

Abstract

A dual glucose and oxygen sensor in a polymer format was developed. The dual sensor composed of a blue emitter as the glucose probe, a red emitter as an oxygen probe, and a yellow emitter as a built-in reference probe which does not respond to either glucose or oxygen. All the three probes were chemically immobilized in a polyacrylamide-based matrix. Therefore, the dual sensor possesses three well separated emission colors and ratiometric approach is applicable for analysis of the glucose and oxygen concentration at biological conditions. The sensor was applied for real-time monitoring of glucose and oxygen consumption of bacterial cells, Escherichia coli (E.coli) and Bacillus subtilis (B.subtilis), and mammalian cells of mouse macrophage J774 and human cervical cancer HeLa cell lines. On the other hand, in order to achieve satisfactory sensing performance for glucose, compositions of the matrices among poly(2-hydroxyethyl methacrylate), polyacrylamide, and poly(6-aminohexyl methacrylamide) which is a linker polymer for grafting the glucose probe, were optimized.

Original languageEnglish (US)
Pages (from-to)9779-9788
Number of pages10
JournalBiomaterials
Volume34
Issue number38
DOIs
StatePublished - Dec 1 2013

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Keywords

  • Dual sensor
  • Glucose sensor
  • Oxygen sensor
  • Ratiometric
  • Sensor

ASJC Scopus subject areas

  • Bioengineering
  • Ceramics and Composites
  • Biophysics
  • Biomaterials
  • Mechanics of Materials

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