A dedicated type II NADPH dehydrogenase performs the penultimate step in the biosynthesis of vitamin K<inf>1</inf> in synechocystis and arabidopsis

Abdelhak Fatihi, Scott Latimer, Stefan Schmollinger, Anna Block, Patrick H. Dussault, Willem Vermaas, Sabeeha S. Merchant, Gilles J. Basset

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Mutation of Arabidopsis thaliana NAD(P)H DEHYDROGENASE C1 (NDC1;At5g08740) results in the accumulation of demethylphylloquinone, a late biosynthetic intermediate of vitamin K<inf>1</inf>. Gene coexpression and phylogenomics analyses showed that conserved functional associations occur between vitamin K biosynthesis and NDC1 homologs throughout the prokaryotic and eukaryotic lineages. Deletion of Synechocystis ndbB, which encodes for one such homolog, resulted in the same defects as those observed in the cyanobacterial demethylnaphthoquinone methyltransferase knockout. Chemical modeling and assay of purified demethylnaphthoquinone methyltransferase demonstrated that, by virtue of the strong electrophilic nature of S-adenosyl-L-methionine, the transmethylation of the demethylated precursor of vitamin K is strictly dependent on the reduced form of its naphthoquinone ring. NDC1 was shown to catalyze such a prerequisite reduction by using NADPH and demethylphylloquinone as substrates and flavine adenine dinucleotide as a cofactor. NDC1 displayed Michaelis-Menten kinetics and was markedly inhibited by dicumarol, a competitive inhibitor of naphthoquinone oxidoreductases. These data demonstrate that the reduction of the demethylnaphthoquinone ring represents an authentic step in the biosynthetic pathway of vitamin K, that this reaction is enzymatically driven, and that a selection pressure is operating to retain type II NAD(P)H dehydrogenases in this process.

Original languageEnglish (US)
Pages (from-to)1730-1741
Number of pages12
JournalPlant Cell
Volume27
Issue number6
DOIs
StatePublished - Jun 1 2015

Fingerprint

Vitamin K 1
Synechocystis
phylloquinone
NADPH Dehydrogenase
vitamin K
Vitamin K
Arabidopsis
NADP (coenzyme)
Naphthoquinones
naphthoquinones
Oxidoreductases
NAD(P)H dehydrogenase (quinone)
methyltransferases
Methyltransferases
biosynthesis
NAD
Dicumarol
S-Adenosylmethionine
enzyme kinetics
S-adenosylmethionine

ASJC Scopus subject areas

  • Plant Science
  • Cell Biology

Cite this

A dedicated type II NADPH dehydrogenase performs the penultimate step in the biosynthesis of vitamin K<inf>1</inf> in synechocystis and arabidopsis. / Fatihi, Abdelhak; Latimer, Scott; Schmollinger, Stefan; Block, Anna; Dussault, Patrick H.; Vermaas, Willem; Merchant, Sabeeha S.; Basset, Gilles J.

In: Plant Cell, Vol. 27, No. 6, 01.06.2015, p. 1730-1741.

Research output: Contribution to journalArticle

Fatihi, Abdelhak ; Latimer, Scott ; Schmollinger, Stefan ; Block, Anna ; Dussault, Patrick H. ; Vermaas, Willem ; Merchant, Sabeeha S. ; Basset, Gilles J. / A dedicated type II NADPH dehydrogenase performs the penultimate step in the biosynthesis of vitamin K<inf>1</inf> in synechocystis and arabidopsis. In: Plant Cell. 2015 ; Vol. 27, No. 6. pp. 1730-1741.
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