A Catalysis-Based Selection for Peroxidase Antibodies with Increased Activity

Jun Yin, Jeremy H. Mills, Peter G. Schultz

Research output: Contribution to journalArticle

21 Scopus citations

Abstract

A biotin-tyramine conjugate (1) was found to covalently cross-link with peroxidase antibody 7G12 upon the catalytic oxidation of the tyramine moiety in the presence of hydrogen peroxide (H2O2). On the basis of this observation, a novel strategy was developed to select mutants of 7G12 Fab with enhanced peroxidase activity from a library of phage displayed antibodies. In such a selection, tyramine is oxidized by hydrogen peroxide in a process catalyzed by peroxidase antibodies displayed on phage. Antibodies with higher peroxidase activity are preferentially labeled with biotin through irreversible adduct formation between oxidized biotin-linked tyramine molecules and phenolic side chains of the antibody. The corresponding phage particles can then be selected via biotin?streptavidin interactions. Using this strategy, phage displayed libraries of antibody 7G12 were selected for higher peroxidase activity. As a result, mutations of antibody 7G12 that led to 10 to 20-fold increases in the peroxidase activity (kcat/Km) were identified, suggesting the validity of this method for the evolution of peroxidase antibodies based directly on catalytic turnover.

Original languageEnglish (US)
Pages (from-to)3006-3007
Number of pages2
JournalJournal of the American Chemical Society
Volume126
Issue number10
DOIs
StatePublished - Mar 17 2004
Externally publishedYes

ASJC Scopus subject areas

  • Catalysis
  • Chemistry(all)
  • Biochemistry
  • Colloid and Surface Chemistry

Fingerprint Dive into the research topics of 'A Catalysis-Based Selection for Peroxidase Antibodies with Increased Activity'. Together they form a unique fingerprint.

  • Cite this