TY - JOUR
T1 - A broad-spectrum antifungal from the marine sponge Hyrtios erecta
AU - Pettit, Robin
AU - McAllister, Shane C.
AU - Pettit, George
AU - Herald, Cherry L.
AU - Johnson, J. Morris
AU - Cichacz, Zbigniew
N1 - Funding Information:
With appreciation we acknowledge the financial assistance provided by a Western Oregon State College Foundation grant, Outstanding Investigator Grant CA44344-01-09 awarded by the Division of Cancer Treatment, the US National Cancer Institute, DHHS, The Arizona Disease Control Research Commission and the Fannie E. Rippel Foundation. We thank Melanie Filiatrault for performing the disk diffusion assays.
PY - 1998/1
Y1 - 1998/1
N2 - Spongistatin 1, a macrocyclic lactone polyether from the marine sponge Hyrtios erecta, was fungicidal for a variety of opportunistic yeasts and filamentous fungi, including strains resistant to amphotericin B, ketoconazole and flucytosine. In broth macrodilution assays, MICs ranged from 0.195 to 12.5 μg/ml, and minimum fungicidal concentrations ranged from 3.12 to 25 μg/ml. Initial disk diffusion screens with six related macrocyclic lactone polyethers from H. erecta and Spirastrella spinispirulifera, revealed that these polyethers were also antifungal. The fungicidal activity of spongistatin 1 was confirmed in killing kinetics studies, where killing of Candida albicans and Cryptococcus neoformans occurred within 6 and 12 h, respectively. During the killing kinetics experiments, non-treated C. albicans maintained the yeast morphology. However, elongated forms resembling germ tubes were the predominant morphologic form in spongistatin 1-treated C. albicans cultures. The spongistatins show promise as potential antifungal agents and as probes to study fungal morphogenesis and nuclear division.
AB - Spongistatin 1, a macrocyclic lactone polyether from the marine sponge Hyrtios erecta, was fungicidal for a variety of opportunistic yeasts and filamentous fungi, including strains resistant to amphotericin B, ketoconazole and flucytosine. In broth macrodilution assays, MICs ranged from 0.195 to 12.5 μg/ml, and minimum fungicidal concentrations ranged from 3.12 to 25 μg/ml. Initial disk diffusion screens with six related macrocyclic lactone polyethers from H. erecta and Spirastrella spinispirulifera, revealed that these polyethers were also antifungal. The fungicidal activity of spongistatin 1 was confirmed in killing kinetics studies, where killing of Candida albicans and Cryptococcus neoformans occurred within 6 and 12 h, respectively. During the killing kinetics experiments, non-treated C. albicans maintained the yeast morphology. However, elongated forms resembling germ tubes were the predominant morphologic form in spongistatin 1-treated C. albicans cultures. The spongistatins show promise as potential antifungal agents and as probes to study fungal morphogenesis and nuclear division.
KW - Antifungal susceptibility
KW - Opportunistic fungi
KW - Spongistatin
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U2 - 10.1016/S0924-8579(97)00044-7
DO - 10.1016/S0924-8579(97)00044-7
M3 - Article
C2 - 9552710
AN - SCOPUS:0031881651
SN - 0924-8579
VL - 9
SP - 147
EP - 152
JO - International Journal of Antimicrobial Agents
JF - International Journal of Antimicrobial Agents
IS - 3
ER -