β-catenin-dependent Wnt signalling controls the epithelial organisation of somites through the activation of paraxis

Claudia Linker, Cynthia Lesbros, Jérôme Gros, Laura W. Burrus, Alan Rawls, Christophe Marcelle

Research output: Contribution to journalArticle

84 Citations (Scopus)

Abstract

The regulation of cell adhesion in epithelia is a fundamental process governing morphogenesis in embryos and a key step in the progression of invasive cancers. Here, we have analysed the molecular pathways controlling the epithelial organisation of somites. Somites are mesodermal epithelial structures of vertebrate embryos that undergo several changes in cell adhesion during early embryonic life. We show that Wnt6 in the ectoderm overlaying the somites, but not Wnt1 in the neighbouring neural tube, is the most likely candidate molecule responsible for the maintenance of the epithelial structure of the dorsal compartment of the somite: the dermomyotome. We characterised the signalling pathway that mediates Wnt6 activity. Our experiments suggest that the Wnt receptor molecule Frizzled7 probably transduces the Wnt6 signal. Intracellularly, this leads to the activation of the β-catenin/LEF1-dependent pathway. Finally, we demonstrate that the bHLH transcription factor paraxis, which was previously shown to be a major player in the epithelial organisation of somites, is a target of the β-catenin signal. We conclude that β-catenin activity, initiated by Wnt6 and mediated by paraxis, is required for the maintenance of the epithelial structure of somites.

Original languageEnglish (US)
Pages (from-to)3895-3905
Number of pages11
JournalDevelopment
Volume132
Issue number17
DOIs
StatePublished - Sep 2005

Fingerprint

Catenins
Somites
adhesion
activation
embryo
morphogenesis
cancer
vertebrate
Cell Adhesion
Embryonic Structures
candidacy
Wnt Receptors
Maintenance
Basic Helix-Loop-Helix Transcription Factors
regulation
Ectoderm
Neural Tube
experiment
Morphogenesis
Vertebrates

Keywords

  • β-catenin
  • Chick
  • Epithelial-mesenchymal transition
  • Paraxis
  • Somite
  • Wnt

ASJC Scopus subject areas

  • Anatomy
  • Cell Biology

Cite this

β-catenin-dependent Wnt signalling controls the epithelial organisation of somites through the activation of paraxis. / Linker, Claudia; Lesbros, Cynthia; Gros, Jérôme; Burrus, Laura W.; Rawls, Alan; Marcelle, Christophe.

In: Development, Vol. 132, No. 17, 09.2005, p. 3895-3905.

Research output: Contribution to journalArticle

Linker, Claudia ; Lesbros, Cynthia ; Gros, Jérôme ; Burrus, Laura W. ; Rawls, Alan ; Marcelle, Christophe. / β-catenin-dependent Wnt signalling controls the epithelial organisation of somites through the activation of paraxis. In: Development. 2005 ; Vol. 132, No. 17. pp. 3895-3905.
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