Wide-field, single-pixel fluorescence imaging with on-chip nanophotonics

Wide-field, single-pixel fluorescence imaging with on-chip nanophotonics Wide-field, single-pixel fluorescence imaging with on-chip nanophotonics We propose a potential solution to fully realize the advantages of both single-pixel and imaging-based approaches. We will construct a wide-field, single-pixel imaging system that uses nanophotonics to control the excitation light and computational inference to form an image. We will achieve this by encoding a unique time- and space-varying intensity pattern onto each point in the sample space, creating pseudo pixels which are generated from a single, polarization-tunable nanophotonic chip. These pseudo pixels eliminate the need to scan the excitation beam in a known pattern to create the exact correspondence between the object and detected photons. The proposed nanophotonic approach has the ability to circumvent the need for complex programmable optical elements, such as spatial light modulators, to generate versatile patterns in the optical excitation wavefront. This has potential to scale down the system hardware requirements and enable the creation of mobile and more widely available 3D fluorescence imaging systems.