Project Details

Description

Single Cell Analysis of Breast Cancer Tumor Heterogeneity Single Cell Analysis of Breast Cancer Tumor Heterogeneity Somatic mutations occur with varying frequencies within primary tumors, yielding diverse and unique clones and subclones of cells at primary and metastatic sites that frequently emerge with resistance to targeted therapy. Currently, there are no single-cell methods available to rapidly analyze the clonal evolution of tumor heterogeneity. Here, we propose a focused project to develop nanomolecular scaffolds for sequencing p53, PIK3CA, and PTEN within single cells in breast cancer without the need for costly single-cell sorting. We will test the nanoparticles in cell lines with defined mutations, and then evaluate heterogeneous co-mutation frequencies within bulk primary tumor cell populations derived from breast cancer patients. This approach will provide a sensitive and cost-effective platform for investigators to rapidly quantitate genomic diversity in solid and liquid tumors for improved targeting of malignant subclones.
StatusFinished
Effective start/end date1/1/1512/31/15

Funding

  • ASU: Mayo Seed Grant: $30,000.00

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