CLAS: Microscale Thermophoresis Facility

Equipment/facility: Facility

  • Location

    United States

Description

MicroScale Thermophoresis (MST) is an easy, fast and precise way to quantify biomolecular interactions. It measures the motion of molecules along microscopic temperature gradients and detects changes in their hydration shell, charge or size. When performing a MST experiment, a microscopic temperature gradient is induced by an infrared laser, and the directed movement of molecules is detected and quantified using either covalently attached dyes, or fluorescent fusion protein. MST detects interactions between any kind of biomolecules thus providing a large application range, from ions and small molecules to high molecular weight and multi-protein complexes.

Fingerprint

Thermophoresis
Thermal gradients
Molecules
Infrared lasers
Biomolecules
Hydration
Proteins
Coloring Agents
Fusion reactions
Molecular weight
Ions
Experiments