TY - JOUR
T1 - β3-Integrins rather than β1-integrins dominate integrin-matrix interactions involved in postinjury smooth muscle cell migration
AU - Slepian, Marvin J.
AU - Massia, Stephen P.
AU - Dehdashti, Behrooz
AU - Fritz, Anne
AU - Whitesell, Luke
PY - 1998/5/12
Y1 - 1998/5/12
N2 - Background - Smooth muscle cell (SMC) migration is a vital component in the response of the arterial wall to revascularization injury. Cell surface integrin-extracellular matrix interactions are essential for cell migration. SMCs express both β1- and β3-integrins. In this study, we examined the relative functional roles of β1- and β3-integrin-matrix interactions in postinjury SMC migration. Methods and Results - Flow cytometry and fluorescence microscopy of migrating SMCs immunostained with anti-β1 and anti-α(v)β3/5 antibodies (Abs) revealed expression of both β1- andβ3- integrins, with β1 observed as linear streaks and β3 found in focal contacts. In a scrape-wound migration assay, anti-β1 Abs (92.0 ± 10.7% of control, P = . 1) and 0.5 mmol/L linear RGD (105 ± 5% of control, P = .2) did not alter SMC migration at 48 hours after injury. β3-Blockade, however, via Abs (anti-β3/5 35.7 ± 4.5% of control, anti-β3 61 ± 12% of control, both P < .001) and cyclic RGD (0.5 mmol/L) (12 ± 10% of control, P < .001) decreased migration. Neither β1- nor β3-inhibition altered postinjury [3H]thymidine incorporation. In the rat carotid injury model, local adventitial polymer-based delivery of radiolabeled linear or cyclic RGD led to uptake and retention of label, for both peptides, over a 72-hour period after injury. Local arterial wall β1-blockade via polymer-based delivery of linear RGD had no effect on SMC migration at 4.5 days (11.5 ± 3.2 versus 12.8 SMCs per x 600 field [control], P = .6) or on neointimal thickening at 14 days (I/M area ratio, 0.664 ± 0.328 versus 1.179 ± 0.324 [control], P = .6) after injury. In contrast, local β3-blockade via cRGD limited migration (0.8 ± 0.8 versus 12.8 ± 4.4 SMCs per x 600 field [control], P < .01) and thickening (I/M area ratio, 0.004 ± 0.008 versus 1.179 ± 0.324 [control], P < .01). Conclusions - In postinjury migrating SMCs, β3- rather than β1-integrin-matrix interactions are of greater functional significance in adhesive processes essential for SMC migration in vitro and in vivo. Blockade of dominant SMC integrin (/33)-matrix interactions may be a valuable approach for limiting injury-induced SMC migration and late arterial renarrowing.
AB - Background - Smooth muscle cell (SMC) migration is a vital component in the response of the arterial wall to revascularization injury. Cell surface integrin-extracellular matrix interactions are essential for cell migration. SMCs express both β1- and β3-integrins. In this study, we examined the relative functional roles of β1- and β3-integrin-matrix interactions in postinjury SMC migration. Methods and Results - Flow cytometry and fluorescence microscopy of migrating SMCs immunostained with anti-β1 and anti-α(v)β3/5 antibodies (Abs) revealed expression of both β1- andβ3- integrins, with β1 observed as linear streaks and β3 found in focal contacts. In a scrape-wound migration assay, anti-β1 Abs (92.0 ± 10.7% of control, P = . 1) and 0.5 mmol/L linear RGD (105 ± 5% of control, P = .2) did not alter SMC migration at 48 hours after injury. β3-Blockade, however, via Abs (anti-β3/5 35.7 ± 4.5% of control, anti-β3 61 ± 12% of control, both P < .001) and cyclic RGD (0.5 mmol/L) (12 ± 10% of control, P < .001) decreased migration. Neither β1- nor β3-inhibition altered postinjury [3H]thymidine incorporation. In the rat carotid injury model, local adventitial polymer-based delivery of radiolabeled linear or cyclic RGD led to uptake and retention of label, for both peptides, over a 72-hour period after injury. Local arterial wall β1-blockade via polymer-based delivery of linear RGD had no effect on SMC migration at 4.5 days (11.5 ± 3.2 versus 12.8 SMCs per x 600 field [control], P = .6) or on neointimal thickening at 14 days (I/M area ratio, 0.664 ± 0.328 versus 1.179 ± 0.324 [control], P = .6) after injury. In contrast, local β3-blockade via cRGD limited migration (0.8 ± 0.8 versus 12.8 ± 4.4 SMCs per x 600 field [control], P < .01) and thickening (I/M area ratio, 0.004 ± 0.008 versus 1.179 ± 0.324 [control], P < .01). Conclusions - In postinjury migrating SMCs, β3- rather than β1-integrin-matrix interactions are of greater functional significance in adhesive processes essential for SMC migration in vitro and in vivo. Blockade of dominant SMC integrin (/33)-matrix interactions may be a valuable approach for limiting injury-induced SMC migration and late arterial renarrowing.
KW - Cell adhesion molecules
KW - Integrins
KW - Restenosis
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U2 - 10.1161/01.CIR.97.18.1818
DO - 10.1161/01.CIR.97.18.1818
M3 - Article
C2 - 9603537
AN - SCOPUS:0032510595
SN - 0009-7322
VL - 97
SP - 1818
EP - 1827
JO - Circulation
JF - Circulation
IS - 18
ER -