Tsafrir Leket-Mor

2017

A heterologous prime-boosting strategy with replicating Vaccinia virus vectors and plant-produced HIV-1 Gag/dgp41 virus-like particles

Meador, L. R., Kessans, S. A., Kilbourne, J., Kibler, K., Pantaleo, G., Roderiguez, M. E., Blattman, J., Jacobs, B. & Leket-Mor, T., Jul 1 2017, In : Virology. 507, p. 242-256 15 p.

Research output: Contribution to journal › Article

Vaccinia virus

Virion

HIV-1

Epitopes

T-Lymphocytes

4 Citations (Scopus)

Bacterial expression, correct membrane targeting and functional folding of the HIV-1 membrane protein Vpu using a periplasmic signal peptide

Deb, A., Johnson, W. A., Kline, A. P., Scott, B. J., Meador, L. R., Srinivas, D., Martin-Garcia, J. M., Dörner, K., Borges, C., Misra, R., Hogue, B., Fromme, P. & Leket-Mor, T., Feb 1 2017, In : PLoS One. 12, 2, e0172529.

Research output: Contribution to journal › Article

viral proteins

Viral Proteins

Protein Sorting Signals

Human immunodeficiency virus 1

signal peptide

6 Citations (Scopus)

Plant-expressed cocaine hydrolase variants of butyrylcholinesterase exhibit altered allosteric effects of cholinesterase activity and increased inhibitor sensitivity

Larrimore, K. E., Kazan, I. C., Kannan, L., Kendle, R. P., Jamal, T., Barcus, M., Bolia, A., Brimijoin, S., Zhan, C. G., Ozkan, S. & Leket-Mor, T., Dec 1 2017, In : Scientific Reports. 7, 1, 10419.

Research output: Contribution to journal › Article

Butyrylcholinesterase

Cholinesterases

Hydrolases

Cocaine

Enzymes

2016

6 Citations (Scopus)

Immunological Characterization of Plant-Based HIV-1 Gag/Dgp41 Virus-Like Particles

Kessans, S. A., Linhart, M. D., Meador, L. R., Kilbourne, J., Hogue, B., Fromme, P., Matoba, N. & Leket-Mor, T., 2016, In : PLoS One. 11, 3, p. e0151842

Research output: Contribution to journal › Article

virus-like particles

Human immunodeficiency virus 1

Viruses

Virion

HIV-1

2015

2 Citations (Scopus)

Biophysical characterization of a vaccine candidate against HIV-1: The transmembrane and membrane proximal domains of HIV-1 gp41 as a maltose binding protein fusion

Gong, Z., Martin-Garcia, J. M., Daskalova, S. M., Craciunescu, F. M., Song, L., Dörner, K., Hansen, D., Yang, J. H., LaBaer, J., Hogue, B., Leket-Mor, T. & Fromme, P., Aug 21 2015, In : PLoS One. 10, 8, e0136507.

Research output: Contribution to journal › Article

Maltose-Binding Proteins

maltose

Human immunodeficiency virus 1

HIV-1

binding proteins

18 Citations (Scopus)

Molecular pharming’s foot in the FDA’s door: Protalix’s trailblazing story

Leket-Mor, T., Nov 29 2015, In : Biotechnology Letters. 37, 11, p. 2147-2150 4 p.

Research output: Contribution to journal › Article

Glucosylceramidase

United States Food and Drug Administration

Biological Products

Drug products

Foot

14 Citations (Scopus)

The case for plant-made veterinary immunotherapeutics

Topp, E., Irwin, R., McAllister, T., Lessard, M., Joensuu, J. J., Kolotilin, I., Conrad, U., Stöger, E., Leket-Mor, T., Warzecha, H., Hall, J. C., McLean, M. D., Cox, E., Devriendt, B., Potter, A., Depicker, A., Virdi, V., Holbrook, L., Doshi, K., Dussault, M. & 5 others, Friendship, R., Yarosh, O., Yoo, H. S., MacDonald, J. & Menassa, R., Sep 29 2015, (Accepted/In press) In : Biotechnology Advances.

Research output: Contribution to journal › Article

Vaccines

Animal Diseases

Animals

Antibiotics

Disease control

2014

5 Citations (Scopus)

Expression, purification and crystallization of CTB-MPR, a candidate mucosal vaccine component against HIV-1

Lee, H. H., Cherni, I., Yu, H., Fromme, R., Doran, J. D., Grotjohann, I., Mittman, M., Basu, S., Deb, A., Dörner, K., Aquila, A., Barty, A., Boutet, S., Chapman, H. N., Doak, R. B., Hunter, M. S., James, D., Kirian, R., Kupitz, C., Lawrence, R. M. & 17 others, Liu, H., Nass, K., Schlichting, I., Schmidt, K., Seibert, M. M., Shoeman, R. L., Spence, J., Stellato, F., Weierstall, U., Williams, G. J., Yoon, C., Wang, D., Zatsepin, N., Hogue, B., Matoba, N., Fromme, P. & Leket-Mor, T., Aug 29 2014, In : IUCrJ. 1, p. 305-317 13 p.

Research output: Contribution to journal › Article

vaccines

human immunodeficiency virus

Vaccines

Crystallization

Viruses

30 Citations (Scopus)

Expression of human butyrylcholinesterase with an engineered glycosylation profile resembling the plasma-derived orthologue

Schneider, J. D., Castilho, A., Neumann, L., Altmann, F., Loos, A., Kannan, L., Leket-Mor, T. & Steinkellner, H., 2014, In : Biotechnology Journal. 9, 4, p. 501-510 10 p.

Research output: Contribution to journal › Article

Butyrylcholinesterase

Glycosylation

Polysaccharides

Proteins

Extracellular Fluid

4 Citations (Scopus)

Meeting report VLPNPV: Session 5: Plant based technology

Meador, L. R. & Leket-Mor, T., Oct 1 2014, In : Human Vaccines and Immunotherapeutics. 10, 10, p. 3068-3073 6 p.

Research output: Contribution to journal › Article

Virion

Technology

Nanoparticles

Virus-Like Particle Vaccines

Plant Viruses

25 Citations (Scopus)

Oligomerization status influences subcellular deposition and glycosylation of recombinant butyrylcholinesterase in Nicotiana benthamiana

Schneider, J. D., Marillonnet, S., Castilho, A., Gruber, C., Werner, S., Mach, L., Klimyuk, V., Leket-Mor, T. & Steinkellner, H., 2014, In : Plant Biotechnology Journal. 12, 7, p. 832-839 8 p.

Research output: Contribution to journal › Article

Butyrylcholinesterase

Nicotiana benthamiana

cholinesterase

glycosylation

Glycosylation

3 Citations (Scopus)

Recombinant expression, purification, and biophysical characterization of the transmembrane and membrane proximal domains of HIV-1 gp41

Gong, Z., Kessans, S. A., Song, L., Dörner, K., Lee, H. H., Meador, L. R., LaBaer, J., Hogue, B., Leket-Mor, T. & Fromme, P., Nov 1 2014, In : Protein Science. 23, 11, p. 1607-1618 12 p.

Research output: Contribution to journal › Article

Purification

HIV-1

Membranes

Neutralizing Antibodies

Glycoproteins

2013

19 Citations (Scopus)

Biological and biochemical characterization of HIV-1 Gag/dgp41 virus-like particles expressed in Nicotiana benthamiana

Kessans, S. A., Linhart, M. D., Matoba, N. & Leket-Mor, T., Aug 2013, In : Plant Biotechnology Journal. 11, 6, p. 681-690 10 p.

Research output: Contribution to journal › Article

virus-like particles

Nicotiana benthamiana

Human immunodeficiency virus 1

Virion

Tobacco

10 Citations (Scopus)

Plants as a source of butyrylcholinesterase variants designed for enhanced cocaine hydrolase activity

Larrimore, K. E., Barcus, M., Kannan, L., Gao, Y., Zhan, C. G., Brimijoin, S. & Leket-Mor, T., Mar 25 2013, In : Chemico-Biological Interactions. 203, 1, p. 217-220 4 p.

Research output: Contribution to journal › Article

Butyrylcholinesterase

Hydrolases

Cocaine

Recurrence

Cocaine-Related Disorders

4 Citations (Scopus)

Reversal of Succinylcholine Induced Apnea with an Organophosphate Scavenging Recombinant Butyrylcholinesterase

Geyer, B. C., Larrimore, K. E., Kilbourne, J., Kannan, L. & Leket-Mor, T., Mar 11 2013, In : PLoS One. 8, 3, e59159.

Research output: Contribution to journal › Article

Butyrylcholinesterase

suxamethonium

apnea

Succinylcholine

Organophosphates

5 Citations (Scopus)

The Arabidopsis thaliana ortholog of a purported maize cholinesterase gene encodes a GDSL-lipase

Muralidharan, M., Buss, K., Larrimore, K. E., Segerson, N. A., Kannan, L. & Leket-Mor, T., 2013, In : Plant Molecular Biology. 81, 6, p. 565-576 12 p.

Research output: Contribution to journal › Article

cholinesterase

Cholinesterases

Acetylcholinesterase

Lipase

Arabidopsis

2012

4 Citations (Scopus)

Nicotinic stimulation induces Tristetraprolin over-production and attenuates inflammation in muscle

Geyer, B. C., Ben Ari, S., Barbash, S., Kilbourne, J., Leket-Mor, T. & Soreq, H., Feb 2012, In : Biochimica et Biophysica Acta - Molecular Cell Research. 1823, 2, p. 368-378 11 p.

Research output: Contribution to journal › Article

Tristetraprolin

Paraoxon

Cholinergic Agents

Inflammation

Muscles

2011

6 Citations (Scopus)

Humoral immunogenicity of an HIV-1 envelope residue 649-684 membrane-proximal region peptide fused to the plague antigen F1-V

Matoba, N., Shah, N. R. & Leket-Mor, T., Aug 5 2011, In : Vaccine. 29, 34, p. 5584-5590 7 p.

Research output: Contribution to journal › Article

Plague

plague

Human immunodeficiency virus 1

HIV-1

Immunization

2010

56 Citations (Scopus)

Plant-derived human butyrylcholinesterase, but not an organophosphorous- compound hydrolyzing variant thereof, protects rodents against nerve agents

Geyer, B. C., Kannan, L., Garnaud, P. E., Broomfield, C. A., Cadieux, C. L., Cherni, I., Hodgins, S. M., Kasten, S. A., Kelley, K., Kilbourne, J., Oliver, Z. P., Otto, T. C., Puffenberger, I., Reeves, T. E., Robbins, N., Woods, R. R., Soreq, H., Lenz, D. E., Cerasoli, D. M. & Leket-Mor, T., Nov 23 2010, In : Proceedings of the National Academy of Sciences of the United States of America. 107, 47, p. 20251-20256 6 p.

Research output: Contribution to journal › Article

Butyrylcholinesterase

Acid Anhydride Hydrolases

Acetylcholinesterase

Pesticides

Rodentia

40 Citations (Scopus)

Transgenic plants as a source for the bioscavenging enzyme, human butyrylcholinesterase

Geyer, B. C., Kannan, L., Cherni, I., Woods, R. R., Soreq, H. & Leket-Mor, T., Oct 2010, In : Plant Biotechnology Journal. 8, 8, p. 873-886 14 p.

Research output: Contribution to journal › Article

Butyrylcholinesterase

cholinesterase

Genetically Modified Plants

transgenic plants

Enzymes

2009

41 Citations (Scopus)

Biochemical and immunological characterization of the plant-derived candidate human immunodeficiency virus type 1 mucosal vaccine CTB-MPR _649-684

Matoba, N., Kajiura, H., Cherni, I., Doran, J. D., Bomsel, M., Fujiyama, K. & Leket-Mor, T., Feb 2009, In : Plant Biotechnology Journal. 7, 2, p. 129-145 17 p.

Research output: Contribution to journal › Article

Human immunodeficiency virus 1

HIV-1

Vaccines

vaccines

G(M1) Ganglioside

2 Citations (Scopus)

P02-10. Plant expression of chimeric Gag/gp41 virus-like particles as a mucosally-targeted subunit vaccine against HIV-1

Kessans, S. A., Frater, J., Matoba, N. & Leket-Mor, T., Sep 22 2009, In : Retrovirology. 6, SUPPL. 3, 1742.

Research output: Contribution to journal › Article

2 Citations (Scopus)

P12-16. Biochemical and immunological characterization of the plant-derived candidate HIV-1 mucosal vaccine CTB-MPR

Matoba, N., Cherni, I., Kessans, S., Frater, J., Preston, K., Bomsel, M. & Leket-Mor, T., Oct 22 2009, In : Retrovirology. 6, SUPPL. 3, 1742.

Research output: Contribution to journal › Article

HIV-1

Vaccines

2008

28 Citations (Scopus)

Acetylcholinesterase-R increases germ cell apoptosis but enhances sperm motility

Mor, I., Sklan, E. H., Podoly, E., Pick, M., Kirschner, M., Yogev, L., Bar-Sheshet Itach, S., Schreiber, L., Geyer, B., Leket-Mor, T., Grisaru, D. & Soreq, H., Apr 2008, In : Journal of Cellular and Molecular Medicine. 12, 2, p. 479-495 17 p.

Research output: Contribution to journal › Article

Sperm Motility

Acetylcholinesterase

Germ Cells

Spermatozoa

Cells

85 Citations (Scopus)

Changes in readthrough acetylcholinesterase expression modulate amyloid-beta pathology

Berson, A., Knobloch, M., Hanan, M., Diamant, S., Sharoni, M., Schuppli, D., Geyer, B. C., Ravid, R., Leket-Mor, T., Nitsch, R. M. & Soreq, H., Jan 2008, In : Brain. 131, 1, p. 109-119 11 p.

Research output: Contribution to journal › Article

Acetylcholinesterase

Amyloid

Pathology

Alzheimer Disease

Cholinergic Agents

29 Citations (Scopus)

Hairy-root organ cultures for the production of human acetylcholinesterase

Woods, R. R., Geyer, B. C. & Leket-Mor, T., Dec 23 2008, In : BMC Biotechnology. 8, 95.

Research output: Contribution to journal › Article

Organ Culture Techniques

Acetylcholinesterase

Enzymes

Genetically Modified Plants

Cell Culture Techniques

8 Citations (Scopus)

Increased organophosphate scavenging in a butyrylcholinesterase mutant

Geyer, B. C., Woods, R. R. & Leket-Mor, T., Sep 25 2008, In : Chemico-Biological Interactions. 175, 1-3, p. 376-379 4 p.

Research output: Contribution to journal › Article

Butyrylcholinesterase

Organophosphates

Scavenging

Cocaine

Hydrolases

16 Citations (Scopus)

Transcytosis-blocking abs elicited by an oligomeric immunogen based on the membrane proximal region of HIV-1 gp41 target non-neutralizing epitopes

Matoba, N., Griffin, T. A., Mittman, M., Doran, J. D., Alfsen, A., Montefiori, D. C., Hanson, C. V., Bornsel, M. & Leket-Mor, T., May 2008, In : Current HIV Research. 6, 3, p. 218-229 12 p.

Research output: Contribution to journal › Article

Transcytosis

Cholera Toxin

HIV-1

Epitopes

Membranes

2007

19 Citations (Scopus)

Adaptive changes in acetylcholinesterase gene expression as mediators of recovery from chemical and biological insults

Evron, T., Greenberg, D., Leket-Mor, T. & Soreq, H., Apr 20 2007, In : Toxicology. 233, 1-3 SPEC. ISS., p. 97-107 11 p.

Research output: Contribution to journal › Article

Paraoxon

Acetylcholinesterase

Gene expression

Lipopolysaccharides

Chemical Warfare Agents

32 Citations (Scopus)

Plant-derived human acetylcholinesterase-R provides protection from lethal organophosphate poisoning and its chronic aftermath

Evron, T., Geyer, B. C., Cherni, I., Muralidharan, M., Kilbourne, J., Fletcher, S. P., Soreq, H. & Leket-Mor, T., Sep 2007, In : FASEB Journal. 21, 11, p. 2961-2969 9 p.

Research output: Contribution to journal › Article

Organophosphate Poisoning

Organophosphates

organophosphorus compounds

Acetylcholinesterase

acetylcholinesterase

17 Citations (Scopus)

Translational control of recombinant human acetylcholinesterase accumulation in plants

Geyer, B. C., Fletcher, S. P., Griffin, T. A., Lopker, M. J., Soreq, H. & Leket-Mor, T., May 30 2007, In : BMC Biotechnology. 7, 27.

Research output: Contribution to journal › Article

Acetylcholinesterase

Codon

Gene Expression

Messenger RNA

GC Rich Sequence

2006

43 Citations (Scopus)

Humoral immune responses by prime-boost heterologous route immunizations with CTB-MPR_649-684, a mucosal subunit HIV/AIDS vaccine candidate

Matoba, N., Geyer, B. C., Kilbourne, J., Alfsen, A., Bomsel, M. & Leket-Mor, T., Jun 5 2006, In : Vaccine. 24, 23, p. 5047-5055 9 p.

Research output: Contribution to journal › Article

AIDS Vaccines

Subunit Vaccines

Humoral Immunity

humoral immunity

cholera toxin

31 Citations (Scopus)

Production of rotavirus-like particles in tomato (Lycopersicon esculentum L.) fruit by expression of capsid proteins VP2 and VP6 and immunological studies

Saldaña, S., Guadarrama, F. E., Olivera Flores, T. D. J., Arias, N., López, S., Arias, C., Ruiz-Medrano, R., Mason, H., Leket-Mor, T., Richter, L., Arntzen, C. J. & Gómez Lim, M. A., 2006, In : Viral Immunology. 19, 1, p. 42-53 12 p.

Research output: Contribution to journal › Article

Rotavirus

Capsid Proteins

Lycopersicon esculentum

Fruit

Virion

2005

4 Citations (Scopus)

(32) Characterizing pea acetylcholinesterase

Muralidharan, M., Soreq, H. & Leket-Mor, T., Dec 15 2005, In : Chemico-Biological Interactions. 157-158, p. 406-407 2 p.

Research output: Contribution to journal › Article

Cholinesterases

Peas

Neurology

Acetylcholinesterase

Cholinergic Agents

24 Citations (Scopus)

Purification of transgenic plant-derived recombinant human acetylcholinesterase-R

Geyer, B. C., Muralidharan, M., Cherni, I., Doran, J., Fletcher, S. P., Evron, T., Soreq, H. & Leket-Mor, T., Dec 15 2005, In : Chemico-Biological Interactions. 157-158, p. 331-334 4 p.

Research output: Contribution to journal › Article

Genetically Modified Plants

Acetylcholinesterase

Purification

Plant Proteins

Cell culture

2004

67 Citations (Scopus)

A mucosally targeted subunit vaccine candidate eliciting HIV-1 transcytosis-blocking Abs

Matoba, N., Magérus, A., Geyer, B. C., Zhang, Y., Muralidharan, M., Alfsen, A., Arntzen, C. J., Bomsel, M. & Leket-Mor, T., Sep 14 2004, In : Proceedings of the National Academy of Sciences of the United States of America. 101, 37, p. 13584-13589 6 p.

Research output: Contribution to journal › Article

Galactosylceramides

Transcytosis

Subunit Vaccines

HIV-1

Mucosal Administration

12 Citations (Scopus)

Tissue distribution of cholinesterases and anticholinesterases in native and transgenic tomato plants

Fletcher, S. P., Geyer, B. C., Smith, A., Evron, T., Joshi, L., Soreq, H. & Leket-Mor, T., May 2004, In : Plant Molecular Biology. 55, 1, p. 33-43 11 p.

Research output: Contribution to journal › Article

cholinesterase

Genetically Modified Plants

Cholinesterase Inhibitors

Cholinesterases

tissue distribution

2003

Erratum: Geminivirus vectors for high-level expression of foreign proteins in plant cells (Biotechnology and Bioengineering (2003) 81 (430-437))

Leket-Mor, T., Moon, Y. S., Palmer, K. E. & Mason, H., Mar 30 2003, In : Biotechnology and Bioengineering. 81, 7, p. 876 1 p.

Research output: Contribution to journal › Article

Biotechnology

Proteins

Plant Cells

Bioengineering

61 Citations (Scopus)

Geminivirus vectors for high-level expression of foreign proteins in plant cells

Leket-Mor, T., Moon, Y. S., Palmer, K. E. & Mason, H., Feb 20 2003, In : Biotechnology and Bioengineering. 81, 4, p. 430-437 8 p.

Research output: Contribution to journal › Article

Geminiviridae

Plant Cells

Viruses

Proteins

Biolistics

2002

183 Citations (Scopus)

Edible plant vaccines: Applications for prophylactic and therapeutic molecular medicine

Mason, H., Warzecha, H., Leket-Mor, T. & Arntzen, C. J., 2002, In : Trends in Molecular Medicine. 8, 7, p. 324-329 6 p.

Research output: Contribution to journal › Article

Edible Vaccines

Molecular Medicine

Edible Plants

Vaccines

Technology

2001

52 Citations (Scopus)

Expression of recombinant human acetylcholinesterase in transgenic tomato plants

Leket-Mor, T., Sternfeld, M., Soreq, H., Arntzen, C. J. & Mason, H., Nov 5 2001, In : Biotechnology and Bioengineering. 75, 3, p. 259-266 8 p.

Research output: Contribution to journal › Article

Genetically Modified Plants

Lycopersicon esculentum

Acetylcholinesterase

Proteins

Enzymes

1998

56 Citations (Scopus)

Perspective: Edible vaccines - A concept coming of age

Leket-Mor, T., Gómez-Lim, M. A. & Palmer, K. E., Nov 1 1998, In : Trends in Microbiology. 6, 11, p. 449-453 5 p.

Research output: Contribution to journal › Article

Edible Vaccines

Plant Antigens

Vaccines

Genetically Modified Plants

Developing Countries

1997

8 Citations (Scopus)

The fate of cytochrome b₅₅₉ during anaerobic photoinhibition and its recovery processes

Leket-Mor, T., Hundal, T., Ohad, I. & Andersson, B., 1997, In : Photosynthesis Research. 53, 2-3, p. 205-213 9 p.

Research output: Contribution to journal › Article

Photosystem II Protein Complex

photoinhibition

cytochromes

photosystem II

Recovery

1995

21 Citations (Scopus)

An unusual organization of the genes encoding cytochrome b₅₅₉ in Chlamydomonas reinhardtii: psbE and psbF genes are separately transcribed from different regions of the plastid chromosome

Leket-Mor, T., Ohad, I., Hirschberg, J. & Pakrasi, H. B., Sep 1995, In : MGG Molecular & General Genetics. 246, 5, p. 600-604 5 p.

Research output: Contribution to journal › Article

Chlamydomonas reinhardtii

Plastids

Chromosomes

Genes

Operon

1994

126 Citations (Scopus)

The ctpA gene encodes the C-terminal processing protease for the D1 protein of the photosystem II reaction center complex

Anbudurai, P. R., Leket-Mor, T., Ohad, I., Shestakov, S. V. & Pakrasi, H. B., Aug 16 1994, In : Proceedings of the National Academy of Sciences of the United States of America. 91, 17, p. 8082-8086 5 p.

Research output: Contribution to journal › Article

Photosystem II Protein Complex

Peptide Hydrolases

Genes

Water

Proteins

1993

10 Citations (Scopus)

The manganese stabilising protein (MSP) of Prochlorothrix hollandica is a hydrophobic membrane-bound protein

Leket-Mor, T., Post, A. F. & Ohad, I., Mar 1 1993, In : BBA - Bioenergetics. 1141, 2-3, p. 206-212 7 p.

Research output: Contribution to journal › Article

Prochlorothrix

Manganese

Membrane Proteins

Cyanobacteria

Photosystem II Protein Complex