XFELs for structure and dynamics in biology

    Research output: Contribution to journalArticle

    35 Citations (Scopus)

    Abstract

    The development and application of the free-electron X-ray laser (XFEL) to structure and dynamics in biology since its inception in 2009 are reviewed. The research opportunities which result from the ability to outrun most radiation-damage effects are outlined, and some grand challenges are suggested. By avoiding the need to cool samples to minimize damage, the XFEL has permitted atomic resolution imaging of molecular processes on the 100fs timescale under near-physiological conditions and in the correct thermal bath in which molecular machines operate. Radiation damage, comparisons of XFEL and synchrotron work, single-particle diffraction, fast solution scattering, pump-probe studies on photosensitive proteins, mix-and-inject experiments, caged molecules, pH jump and other reaction-initiation methods, and the study of molecular machines are all discussed. Sample-delivery methods and data-analysis algorithms for the various modes, from serial femtosecond crystallography to fast solution scattering, fluctuation X-ray scattering, mixing jet experiments and single-particle diffraction, are also reviewed.

    Original languageEnglish (US)
    Pages (from-to)322-339
    Number of pages18
    JournalIUCrJ
    Volume4
    DOIs
    StatePublished - 2017

    Fingerprint

    Radiation damage
    biology
    Diffraction
    X-Rays
    Scattering
    X ray lasers
    radiation damage
    Crystallography
    Synchrotrons
    Molecular Imaging
    Free electron lasers
    Radiation Effects
    X ray scattering
    Baths
    scattering
    Lasers
    Hot Temperature
    Experiments
    Pumps
    Electrons

    Keywords

    • biology
    • dynamics
    • structure
    • X-ray lasers
    • XFELs

    ASJC Scopus subject areas

    • Chemistry(all)
    • Biochemistry
    • Materials Science(all)
    • Condensed Matter Physics

    Cite this

    XFELs for structure and dynamics in biology. / Spence, John.

    In: IUCrJ, Vol. 4, 2017, p. 322-339.

    Research output: Contribution to journalArticle

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