Xenopus laevis egg jelly coats consist of small diffusible proteins bound to a complex system of structurally stable networks composed of high-molecular-weight glycoconjugates

Barry S. Bonnell, Donnie Reinhart, Douglas E. Chandler

Research output: Contribution to journalArticle

47 Citations (Scopus)

Abstract

The extracellular matrix surrounding Xenopus laevis eggs includes three morphologically distinct jelly layers designated J1, J2, and J3 from the innermost to outermost. Previously, using the quick-freeze, deep-etch, rotary-shadow technique, we found that each layer has a unique fibrillogranular ultrastructure. In this study, we show that the fibrillar network is composed of high-molecular-weight glycoconjugates, while the globular material consists of low-molecular-weight proteins some of which are released into the aqueous medium. Analysis by SDS-PAGE and differential staining of individually dissected jelly layers shows that both J1 and J2 contain three high-molecular-weight, acidic, Alcian blue-staining components (450, 630, and 900 kDa), while 13 contains two high-molecular-weight components that stain with PAS but not with Alcian blue. Each jelly layer also contains low-molecular-weight proteins from 75 to 250 kDa that do not stain with PAS or Alcian blue. Chromatography of whole egg jelly on a Sephacryl 500 column resulted in isolation of the major Alcian blue staining band (630 kDa) which eluted first, and two PAS staining bands which eluted second. Rotary-shadowing demonstrated that these high-molecular-weight glycoconjugates are long and branched, suggesting that they are major constituents of the jelly fiber network. SDS-PAGE analysis shows that these networks are stable for at least 16 hr after eggs are oviposited. In contrast, the low-molecular-weight globular proteins which constitute 30% of the total jelly protein are steadily released into the surrounding medium.

Original languageEnglish (US)
Pages (from-to)32-42
Number of pages11
JournalDevelopmental Biology
Volume174
Issue number1
DOIs
StatePublished - 1996

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Glycoconjugates
Xenopus laevis
Ovum
Molecular Weight
Alcian Blue
Proteins
Staining and Labeling
Eggs
Polyacrylamide Gel Electrophoresis
Coloring Agents
Extracellular Matrix
Chromatography

ASJC Scopus subject areas

  • Developmental Biology

Cite this

Xenopus laevis egg jelly coats consist of small diffusible proteins bound to a complex system of structurally stable networks composed of high-molecular-weight glycoconjugates. / Bonnell, Barry S.; Reinhart, Donnie; Chandler, Douglas E.

In: Developmental Biology, Vol. 174, No. 1, 1996, p. 32-42.

Research output: Contribution to journalArticle

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