X-ray laser diffraction for structure determination of the rhodopsin-arrestin complex

X. Edward Zhou, Xiang Gao, Anton Barty, Yanyong Kang, Yuanzheng He, Wei Liu, Andrii Ishchenko, Thomas A. White, Oleksandr Yefanov, Gye Won Han, Qingping Xu, Parker W. De Waal, Kelly M. Suino-Powell, Sébastien Boutet, Garth J. Williams, Meitian Wang, Dianfan Li, Martin Caffrey, Henry N. Chapman, John Spence & 6 others Petra Fromme, Uwe Weierstall, Raymond C. Stevens, Vadim Cherezov, Karsten Melcher, H. Eric Xu

    Research output: Contribution to journalArticle

    19 Citations (Scopus)

    Abstract

    Serial femtosecond X-ray crystallography (SFX) using an X-ray free electron laser (XFEL) is a recent advancement in structural biology for solving crystal structures of challenging membrane proteins, including G-protein coupled receptors (GPCRs), which often only produce microcrystals. An XFEL delivers highly intense X-ray pulses of femtosecond duration short enough to enable the collection of single diffraction images before significant radiation damage to crystals sets in. Here we report the deposition of the XFEL data and provide further details on crystallization, XFEL data collection and analysis, structure determination, and the validation of the structural model. The rhodopsin-arrestin crystal structure solved with SFX represents the first near-atomic resolution structure of a GPCR-arrestin complex, provides structural insights into understanding of arrestin-mediated GPCR signaling, and demonstrates the great potential of this SFX-XFEL technology for accelerating crystal structure determination of challenging proteins and protein complexes.

    Original languageEnglish (US)
    Article number201621
    JournalScientific data
    Volume3
    DOIs
    StatePublished - Apr 12 2016

    Fingerprint

    X ray lasers
    X-ray Laser
    Free Electron Laser
    Free electron lasers
    Femtosecond
    Diffraction
    G Protein
    Proteins
    Crystal Structure
    X ray crystallography
    Receptor
    Crystal structure
    Protein
    Radiation Damage
    Membrane Protein
    Structural Model
    Crystallization
    Microcrystals
    structural model
    Radiation damage

    ASJC Scopus subject areas

    • Education
    • Library and Information Sciences
    • Computer Science Applications
    • Information Systems
    • Statistics, Probability and Uncertainty
    • Statistics and Probability

    Cite this

    X-ray laser diffraction for structure determination of the rhodopsin-arrestin complex. / Zhou, X. Edward; Gao, Xiang; Barty, Anton; Kang, Yanyong; He, Yuanzheng; Liu, Wei; Ishchenko, Andrii; White, Thomas A.; Yefanov, Oleksandr; Han, Gye Won; Xu, Qingping; De Waal, Parker W.; Suino-Powell, Kelly M.; Boutet, Sébastien; Williams, Garth J.; Wang, Meitian; Li, Dianfan; Caffrey, Martin; Chapman, Henry N.; Spence, John; Fromme, Petra; Weierstall, Uwe; Stevens, Raymond C.; Cherezov, Vadim; Melcher, Karsten; Xu, H. Eric.

    In: Scientific data, Vol. 3, 201621, 12.04.2016.

    Research output: Contribution to journalArticle

    Zhou, XE, Gao, X, Barty, A, Kang, Y, He, Y, Liu, W, Ishchenko, A, White, TA, Yefanov, O, Han, GW, Xu, Q, De Waal, PW, Suino-Powell, KM, Boutet, S, Williams, GJ, Wang, M, Li, D, Caffrey, M, Chapman, HN, Spence, J, Fromme, P, Weierstall, U, Stevens, RC, Cherezov, V, Melcher, K & Xu, HE 2016, 'X-ray laser diffraction for structure determination of the rhodopsin-arrestin complex' Scientific data, vol. 3, 201621. https://doi.org/10.1038/sdata.2016.21
    Zhou, X. Edward ; Gao, Xiang ; Barty, Anton ; Kang, Yanyong ; He, Yuanzheng ; Liu, Wei ; Ishchenko, Andrii ; White, Thomas A. ; Yefanov, Oleksandr ; Han, Gye Won ; Xu, Qingping ; De Waal, Parker W. ; Suino-Powell, Kelly M. ; Boutet, Sébastien ; Williams, Garth J. ; Wang, Meitian ; Li, Dianfan ; Caffrey, Martin ; Chapman, Henry N. ; Spence, John ; Fromme, Petra ; Weierstall, Uwe ; Stevens, Raymond C. ; Cherezov, Vadim ; Melcher, Karsten ; Xu, H. Eric. / X-ray laser diffraction for structure determination of the rhodopsin-arrestin complex. In: Scientific data. 2016 ; Vol. 3.
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    AU - He, Yuanzheng

    AU - Liu, Wei

    AU - Ishchenko, Andrii

    AU - White, Thomas A.

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    AU - Xu, Qingping

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    AU - Li, Dianfan

    AU - Caffrey, Martin

    AU - Chapman, Henry N.

    AU - Spence, John

    AU - Fromme, Petra

    AU - Weierstall, Uwe

    AU - Stevens, Raymond C.

    AU - Cherezov, Vadim

    AU - Melcher, Karsten

    AU - Xu, H. Eric

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