X-ray diffraction analysis and molecular-replacement solution of the cyan fluorescent protein dsFP483

Meitian Wang, Hetal N. Patel, Rebekka Wachter

Research output: Contribution to journalArticle

2 Scopus citations

Abstract

A novel cyan fluorescent protein, dsFP483 from the coral Discosoma striata, has been crystallized. Diffraction data were collected to 2.1 Å and processed in space group C2. Molecular-replacement methods were applied using the closely related red fluorescent protein DsRed as a search model. The asymmetric unit appears to contain six protein molecules (1.5 tetramers), five of which (83%) could be located by the molecular-replacement searches.

Original languageEnglish (US)
Pages (from-to)922-924
Number of pages3
JournalActa Crystallographica Section F: Structural Biology and Crystallization Communications
Volume61
Issue number10
DOIs
StatePublished - Dec 1 2005

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ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Genetics
  • Condensed Matter Physics

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