Abstract
Autotransporters (ATs) represent a superfamily of proteins produced by a variety of pathogenic bacteria, which include the pathogenic groups of Escherichia coli (E. coli) associated with gastrointestinal and urinary tract infections. We present the first X-ray structure of the passenger domain from the Plasmid-encoded toxin (Pet) a 100 kDa protein at 2.3 Å resolution which is a cause of acute diarrhea in both developing and industrialized countries. Pet is a cytoskeleton-altering toxin that induces loss of actin stress fibers. While Pet (pdb code: 4OM9) shows only a sequence identity of 50% compared to the closest related protein sequence, extracellular serine protease plasmid (EspP) the structural features of both proteins are conserved. A closer structural look reveals that Pet contains a β-pleaded sheet at the sequence region of residues 181-190, the corresponding structural domain in EspP consists of a coiled loop. Secondary, the Pet passenger domain features a more pronounced beta sheet between residues 135 and 143 compared to the structure of EspP.
Original language | English (US) |
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Pages (from-to) | 439-444 |
Number of pages | 6 |
Journal | Biochemical and Biophysical Research Communications |
Volume | 445 |
Issue number | 2 |
DOIs | |
State | Published - Mar 7 2014 |
Keywords
- 4OM9
- Autotransporter
- EAEC
- Passenger domain
- Plasmid encoded toxin(Pet)
- SPATE
ASJC Scopus subject areas
- Biophysics
- Biochemistry
- Molecular Biology
- Cell Biology
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X-Ray Crystal Structure of the passenger domain of Plasmid encoded toxin, an Autrotansporter Enterotoxin from enteroaggregative Escherichia coli (EAEC)
Domingo Meza-Aguilar, M. J. (Contributor), Fromme, P. (Contributor), Torres-Larios, A. (Contributor), Mendoza-Hernández, G. (Contributor), Hernandez-Chiñas, U. (Contributor), Arreguin-Espinosa De Los Monteros, R. A. (Contributor), Eslava Campos, C. A. (Contributor) & Fromme, R. (Contributor), Protein Data Bank (PDB), Mar 12 2014
DOI: 10.2210/pdb4OM9, https://www.wwpdb.org/pdb?id=pdb_00004om9
Dataset