TY - JOUR
T1 - X chromosome inactivation in the human placenta is patchy and distinct from adult tissues
AU - Phung, Tanya N.
AU - Olney, Kimberly C.
AU - Pinto, Brendan J.
AU - Silasi, Michelle
AU - Perley, Lauren
AU - O'Bryan, Jane
AU - Kliman, Harvey J.
AU - Wilson, Melissa A.
N1 - Funding Information:
We acknowledge Research Computing at Arizona State University for providing high-performance computing and storage resources that have contributed to the research results reported within this paper (http://www.researchcomputing.asu.edu). We would also like to thank Heini Natri and Wilson lab members for helpful feedback on the manuscript. This work was supported by the National Institute of General Medical Sciences (NIGMS) of the National Institutes of Health grant R35GM124827 to M.A.W. This research was supported by The Yale University Reproductive Sciences Data and Specimen Biorepository, HIC#1309012696, a component of the Department of Obstetrics, Gynecology & Reproductive Sciences, Yale School of Medicine, New Haven, CT. Research reported in this publication was supported by the Eunice Kennedy Shriver National Institute Of Child Health & Human Development of the National Institutes of Health under award number F31HD101252 to K.C.O. K.C.O. was additionally supported by ARCS Spetzler Scholar. The authors declare no competing interests.
Funding Information:
We acknowledge Research Computing at Arizona State University for providing high-performance computing and storage resources that have contributed to the research results reported within this paper ( http://www.researchcomputing.asu.edu ). We would also like to thank Heini Natri and Wilson lab members for helpful feedback on the manuscript. This work was supported by the National Institute of General Medical Sciences (NIGMS) of the National Institutes of Health grant R35GM124827 to M.A.W. This research was supported by The Yale University Reproductive Sciences Data and Specimen Biorepository , HIC#1309012696 , a component of the Department of Obstetrics, Gynecology & Reproductive Sciences, Yale School of Medicine , New Haven, CT. Research reported in this publication was supported by the Eunice Kennedy Shriver National Institute Of Child Health & Human Development of the National Institutes of Health under award number F31HD101252 to K.C.O. K.C.O. was additionally supported by ARCS Spetzler Scholar.
Publisher Copyright:
© 2022 The Authors
PY - 2022/7/14
Y1 - 2022/7/14
N2 - In humans, one of the X chromosomes in genetic females is inactivated by a process called X chromosome inactivation (XCI). Variation in XCI across the placenta may contribute to observed sex differences and variability in pregnancy outcomes. However, XCI has predominantly been studied in human adult tissues. Here, we sequenced and analyzed DNA and RNA from two locations from 30 full-term pregnancies. Implementing an allele-specific approach to examine XCI, we report evidence that XCI in the human placenta is patchy, with large patches of either maternal or paternal X chromosomes inactivated. Further, using similar measurements, we show that this is in contrast to adult tissues, which generally exhibit mosaic X inactivation, where bulk samples exhibit both maternal and paternal X chromosome expression. Further, by comparing skewed samples in placenta and adult tissues, we identify genes that are uniquely inactivated or expressed in the placenta compared with adult tissues, highlighting the need for tissue-specific maps of XCI.
AB - In humans, one of the X chromosomes in genetic females is inactivated by a process called X chromosome inactivation (XCI). Variation in XCI across the placenta may contribute to observed sex differences and variability in pregnancy outcomes. However, XCI has predominantly been studied in human adult tissues. Here, we sequenced and analyzed DNA and RNA from two locations from 30 full-term pregnancies. Implementing an allele-specific approach to examine XCI, we report evidence that XCI in the human placenta is patchy, with large patches of either maternal or paternal X chromosomes inactivated. Further, using similar measurements, we show that this is in contrast to adult tissues, which generally exhibit mosaic X inactivation, where bulk samples exhibit both maternal and paternal X chromosome expression. Further, by comparing skewed samples in placenta and adult tissues, we identify genes that are uniquely inactivated or expressed in the placenta compared with adult tissues, highlighting the need for tissue-specific maps of XCI.
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U2 - 10.1016/j.xhgg.2022.100121
DO - 10.1016/j.xhgg.2022.100121
M3 - Article
AN - SCOPUS:85132439326
VL - 3
JO - Human Genetics and Genomics Advances
JF - Human Genetics and Genomics Advances
SN - 2666-2477
IS - 3
M1 - 100121
ER -