Wnt3a links left-right determination with segmentation and anteroposterior axis elongation

Masa Aki Nakaya, Kristin Biris, Tadasuke Tsukiyama, Shaulan Jaime, J. Alan Rawls, Terry P. Yamaguchi

Research output: Contribution to journalArticle

122 Citations (Scopus)

Abstract

The alignment of the left-right (LR) body axis relative to the anteroposterior (AP) and dorsoventral (DV) axes is central to the organization of the vertebrate body plan and is controlled by the node/organizer. Somitogenesis plays a key role in embryo morphogenesis as a principal component of AP elongation. How morphogenesis is coupled to axis specification is not well understood. We demonstrate that Wnt3a is required for LR asymmetry. Wnt3a activates the Delta/Notch pathway to regulate perinodal expression of the left determinant Nodal, while simultaneously controlling the segmentation clock and the molecular oscillations of the Wnt/β-catenin and Notch pathways. We provide evidence that Wnt3a, expressed in the primitive streak and dorsal posterior node, acts as a long-range signaling molecule, directly regulating target gene expression throughout the node and presomitic mesoderm. Wnt3a may also modulate the symmetry-breaking activity of mechanosensory cilia in the node. Thus, Wnt3a links the segmentation clock and AP axis elongation with key left-determining events, suggesting that Wnt3a is an integral component of the trunk organizer.

Original languageEnglish (US)
Pages (from-to)5425-5436
Number of pages12
JournalDevelopment
Volume132
Issue number24
DOIs
StatePublished - Dec 2005

Fingerprint

morphogenesis
Morphogenesis
segmentation
Primitive Streak
Catenins
Cilia
embryo
Mesoderm
asymmetry
gene expression
symmetry
Vertebrates
vertebrate
Embryonic Structures
oscillation
determinants
Gene Expression
organization
event
evidence

Keywords

  • Left-right determination
  • Mouse
  • Wnt3a

ASJC Scopus subject areas

  • Anatomy
  • Cell Biology

Cite this

Nakaya, M. A., Biris, K., Tsukiyama, T., Jaime, S., Rawls, J. A., & Yamaguchi, T. P. (2005). Wnt3a links left-right determination with segmentation and anteroposterior axis elongation. Development, 132(24), 5425-5436. https://doi.org/10.1242/dev.02149

Wnt3a links left-right determination with segmentation and anteroposterior axis elongation. / Nakaya, Masa Aki; Biris, Kristin; Tsukiyama, Tadasuke; Jaime, Shaulan; Rawls, J. Alan; Yamaguchi, Terry P.

In: Development, Vol. 132, No. 24, 12.2005, p. 5425-5436.

Research output: Contribution to journalArticle

Nakaya, MA, Biris, K, Tsukiyama, T, Jaime, S, Rawls, JA & Yamaguchi, TP 2005, 'Wnt3a links left-right determination with segmentation and anteroposterior axis elongation', Development, vol. 132, no. 24, pp. 5425-5436. https://doi.org/10.1242/dev.02149
Nakaya, Masa Aki ; Biris, Kristin ; Tsukiyama, Tadasuke ; Jaime, Shaulan ; Rawls, J. Alan ; Yamaguchi, Terry P. / Wnt3a links left-right determination with segmentation and anteroposterior axis elongation. In: Development. 2005 ; Vol. 132, No. 24. pp. 5425-5436.
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