TY - GEN
T1 - Whole genome transcriptional profiling of the mouse frontal cortex following repeated acamprosate administration
AU - Hicks, Megan P.
AU - Olive, M. Foster
PY - 2009
Y1 - 2009
N2 - Acamprosate (calcium acetylhomotaurinate) has been used for the treatment of alcoholism for over 20 years, and while early evidence suggested it modulates the function of the N-methyl-D-aspartate (NMDA) glutamate receptor subtype, its precise mechanism of action has yet to be determined. In order to further understand how acamprosate acts within the central nervous system, we conducted a whole genome microarray study of the mouse frontal cortex following repeated administration of saline or acamprosate (200 mg/kg i.p.) twice daily for 5 days. Differental gene expression analysis revealed that the expression of a total of 113 genes was changed as a result of acamprosate treatment (53 up-regulated, 60 down-regulated, p<0.01 for each gene). Changes were observed in genes encoding proteins involved in regulation of transcription, post-translational modification, cellular metabolism, neurotransmission, cell-cell communication, lipid signaling, protein phosphorylation, immune cell function, structural function, and appetite regulation. These findings indicate that acamprosate induces changes in the expression of numerous genes in the frontal cortex, and further investigation is needed into which of these gene or genes contribute to its mechanism of action.
AB - Acamprosate (calcium acetylhomotaurinate) has been used for the treatment of alcoholism for over 20 years, and while early evidence suggested it modulates the function of the N-methyl-D-aspartate (NMDA) glutamate receptor subtype, its precise mechanism of action has yet to be determined. In order to further understand how acamprosate acts within the central nervous system, we conducted a whole genome microarray study of the mouse frontal cortex following repeated administration of saline or acamprosate (200 mg/kg i.p.) twice daily for 5 days. Differental gene expression analysis revealed that the expression of a total of 113 genes was changed as a result of acamprosate treatment (53 up-regulated, 60 down-regulated, p<0.01 for each gene). Changes were observed in genes encoding proteins involved in regulation of transcription, post-translational modification, cellular metabolism, neurotransmission, cell-cell communication, lipid signaling, protein phosphorylation, immune cell function, structural function, and appetite regulation. These findings indicate that acamprosate induces changes in the expression of numerous genes in the frontal cortex, and further investigation is needed into which of these gene or genes contribute to its mechanism of action.
KW - Acamprosate
KW - Alcoholism
KW - DNA
KW - Frontal cortex
KW - Microarray
KW - Pharmacotherapy
UR - http://www.scopus.com/inward/record.url?scp=74949093571&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=74949093571&partnerID=8YFLogxK
M3 - Conference contribution
AN - SCOPUS:74949093571
SN - 9789604741106
T3 - Proceedings of the 2nd WSEAS International Conference on Biomedical Electronics and Biomedical Informatics, BEBI '09
SP - 180
EP - 183
BT - Proceedings of the 2nd WSEAS International Conference on Biomedical Electronics and Biomedical Informatics, BEBI '09
T2 - 2nd WSEAS International Conference on Biomedical Electronics and Biomedical Informatics, BEBI '09
Y2 - 20 August 2009 through 22 August 2009
ER -