Whole-cell patch-clamp electrophysiology to study ionotropic glutamatergic receptors and their roles in addiction

Jonna M. Leyrer-Jackson, Michael Olive, Cassandra Gipson-Reichardt

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Development of the whole-cell patch-clamp electrophysiology technique has allowed for enhanced visualization and experimentation of ionic currents in neurons of mammalian tissue with high spatial and temporal resolution. Electrophysiology has become an exceptional tool for identifying single cellular mechanisms underlying behavior. Specifically, the role of glutamatergic signaling through α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) and N-methyl-d-aspartate (NMDA) receptors underlying behavior has been extensively studied. Here we will discuss commonly used protocols and techniques for performing whole-cell patch-clamp recordings and exploring AMPA and NMDA receptor-mediated glutamatergic responses and alterations in the context of substance abuse.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages107-135
Number of pages29
DOIs
StatePublished - Jan 1 2019

Publication series

NameMethods in Molecular Biology
Volume1941
ISSN (Print)1064-3745

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Keywords

  • Addiction
  • AMPA
  • Electrophysiology
  • Glutamatergic receptors
  • NMDA
  • Patch clamp
  • Substance abuse

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Leyrer-Jackson, J. M., Olive, M., & Gipson-Reichardt, C. (2019). Whole-cell patch-clamp electrophysiology to study ionotropic glutamatergic receptors and their roles in addiction. In Methods in Molecular Biology (pp. 107-135). (Methods in Molecular Biology; Vol. 1941). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-9077-1_9