Whole blood gene expression profiles in insulin resistant Latinos with the metabolic syndrome

Samantha E. Tangen, Darwin Tsinajinnie, Martha Nuñez, Gabriel Shaibi, Lawrence J. Mandarino, Dawn K. Coletta

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Although insulin resistance in skeletal muscle is well-characterized, the role of circulating whole blood in the metabolic syndrome phenotype is not well understood. We set out to test the hypothesis that genes involved in inflammation, insulin signaling and mitochondrial function would be altered in expression in the whole blood of individuals with metabolic syndrome. We further wanted to examine whether similar relationships that we have found previously in skeletal muscle exist in peripheral whole blood cells. All subjects (n=184) were Latino descent from the Arizona Insulin Resistance registry. Subjects were classified based on the metabolic syndrome phenotype according to the National Cholesterol Education Program's Adult Treatment Panel III. Of the 184 Latino subjects in the study, 74 were classified with the metabolic syndrome and 110 were without. Whole blood gene expression profiling was performed using the Agilent 4x44K Whole Human Genome Microarray. Whole blood microarray analysis identified 1,432 probes that were altered in expression ≥1.2 fold and P<0.05 after Benjamini-Hochberg in the metabolic syndrome subjects. KEGG pathway analysis revealed significant enrichment for pathways including ribosome, oxidative phosphorylation and MAPK signaling (all Benjamini-Hochberg P<0.05). Whole blood mRNA expression changes observed in the microarray data were confirmed by quantitative RT-PCR. Transcription factor binding motif enrichment analysis revealed E2F1, ELK1, NF-kappaB, STAT1 and STAT3 significantly enriched after Bonferroni correction (all P<0.05). The results of the present study demonstrate that whole blood is a useful tissue for studying the metabolic syndrome and its underlying insulin resistance although the relationship between blood and skeletal muscle differs.

Original languageEnglish (US)
Article numbere84002
JournalPLoS One
Volume8
Issue number12
DOIs
StatePublished - Dec 17 2013

Fingerprint

metabolic syndrome
Transcriptome
Hispanic Americans
Gene expression
insulin resistance
Blood
Insulin
gene expression
blood
Microarrays
skeletal muscle
Insulin Resistance
Skeletal Muscle
Muscle
Genes
Phenotype
phenotype
transcription factor NF-kappa B
oxidative phosphorylation
NF-kappa B

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Tangen, S. E., Tsinajinnie, D., Nuñez, M., Shaibi, G., Mandarino, L. J., & Coletta, D. K. (2013). Whole blood gene expression profiles in insulin resistant Latinos with the metabolic syndrome. PLoS One, 8(12), [e84002]. https://doi.org/10.1371/journal.pone.0084002

Whole blood gene expression profiles in insulin resistant Latinos with the metabolic syndrome. / Tangen, Samantha E.; Tsinajinnie, Darwin; Nuñez, Martha; Shaibi, Gabriel; Mandarino, Lawrence J.; Coletta, Dawn K.

In: PLoS One, Vol. 8, No. 12, e84002, 17.12.2013.

Research output: Contribution to journalArticle

Tangen, Samantha E. ; Tsinajinnie, Darwin ; Nuñez, Martha ; Shaibi, Gabriel ; Mandarino, Lawrence J. ; Coletta, Dawn K. / Whole blood gene expression profiles in insulin resistant Latinos with the metabolic syndrome. In: PLoS One. 2013 ; Vol. 8, No. 12.
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