When less means more: Dehydration improves innate immunity in rattlesnakes

George A. Brusch, Dale Denardo

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Immune function can vary based on availability of resources, and most studies of such influences have focused on the co-investment of energy into immune and other physiological functions. When energy resources are limited, trade-offs exist, which can compromise immunity for other functions. As with energy, water limitation can also alter various physiological processes, yet water has received little consideration for its possible role in modulating immune functions. We examined the relationship between immunocompetence and hydration state using the western diamond-backed rattlesnake (Crotalus atrox). This species is known to undergo substantial seasonal fluctuations in water availability with extreme limitations during the hot-dry season. We collected blood samples from freeranging C. atrox to compare osmolality and innate immune function (lysis, agglutination and bacterial growth inhibition) during the milder and relatively moister early spring season, the hot-dry season and the hot-wet season. To isolate effects of dehydration from other possible seasonal influences, we complemented this field study with a laboratory study in which we withheld food and water from individually housed adult C. atrox for up to 16 weeks. We collected blood samples from each snake as it dehydrated and collected a final sample after the snake was given water ad libitum at the end of the experiment. Our results demonstrate that C. atrox experience significant dehydration during the hot-dry season, and that, in general, innate immune function is highly correlated with osmolality, whether natural or artificially manipulated.

Original languageEnglish (US)
Pages (from-to)2287-2295
Number of pages9
JournalJournal of Experimental Biology
Volume220
Issue number12
DOIs
StatePublished - Jun 15 2017

Fingerprint

Crotalus
Crotalus atrox
immunity
Dehydration
Innate Immunity
dehydration
dry season
warm season
snake
Water
blood
Snakes
immunocompetence
osmolality
spring (season)
water
Osmolar Concentration
snakes
lysis
energy resource

Keywords

  • Hydration
  • Immune function
  • Immunocompetence
  • Osmotic stress
  • Water limitations

ASJC Scopus subject areas

  • Medicine(all)
  • Physiology
  • Ecology, Evolution, Behavior and Systematics
  • Aquatic Science
  • Molecular Biology
  • Animal Science and Zoology
  • Insect Science

Cite this

When less means more : Dehydration improves innate immunity in rattlesnakes. / Brusch, George A.; Denardo, Dale.

In: Journal of Experimental Biology, Vol. 220, No. 12, 15.06.2017, p. 2287-2295.

Research output: Contribution to journalArticle

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