TY - JOUR
T1 - Waveguide coupled surface plasmon resonance imaging measurement and high-throughput analysis of bio-interaction
AU - Song, Lusheng
AU - Wang, Zhiyou
AU - Zhou, Dasu
AU - Nand, Amita
AU - Li, Shaopeng
AU - Guo, Bihong
AU - Wang, Yanmei
AU - Cheng, Zhiqiang
AU - Zhou, Wenfei
AU - Zheng, Zheng
AU - Zhu, Jinsong
N1 - Funding Information:
This research was supported under 973 Program ( 2009CB930702 ), NSFC under Grant ( 61077064 / 60921001 ) and Program of International S&T Cooperation ( 2010DFB33880 ).
Copyright:
Copyright 2013 Elsevier B.V., All rights reserved.
PY - 2013
Y1 - 2013
N2 - A novel surface plasmon resonance imaging (SPRi) biosensor based on waveguide coupled surface plasmon resonance (WCSPR) measurements is reported. Simply fabricated by gold evaporation deposition and polymer spin-coating, the WCSPR chips can be easily adapted on an commercial SPRi monitoring instrument. The chips were designed by using Fresnel method and fabricated according to its optimized design, the calculation and experimental results fits well. Over an 8 mm × 8 mm rectangle imaging area, the WCSPR chips realized about 52% increase of sensitivity, 57% enhancement of resolution and 55% improvement of signal to noise ratio compared to conventional SPR chips. Furthermore, high throughput protein-protein interaction analyses based on WCSPR chips were successfully accomplished by applying Protein A and rabbit antibody interaction in an array format. The reflectivity responses observed from the WCSPR chips were about 50% higher than the conventional SPR chip while the binding kinetic parameters were within the same range as with conventional SPR chip.
AB - A novel surface plasmon resonance imaging (SPRi) biosensor based on waveguide coupled surface plasmon resonance (WCSPR) measurements is reported. Simply fabricated by gold evaporation deposition and polymer spin-coating, the WCSPR chips can be easily adapted on an commercial SPRi monitoring instrument. The chips were designed by using Fresnel method and fabricated according to its optimized design, the calculation and experimental results fits well. Over an 8 mm × 8 mm rectangle imaging area, the WCSPR chips realized about 52% increase of sensitivity, 57% enhancement of resolution and 55% improvement of signal to noise ratio compared to conventional SPR chips. Furthermore, high throughput protein-protein interaction analyses based on WCSPR chips were successfully accomplished by applying Protein A and rabbit antibody interaction in an array format. The reflectivity responses observed from the WCSPR chips were about 50% higher than the conventional SPR chip while the binding kinetic parameters were within the same range as with conventional SPR chip.
KW - High throughput
KW - Kinetic analysis
KW - Sensitivity
KW - Signal to noise ratio
KW - Waveguide coupled surface plasmon resonance imaging
UR - http://www.scopus.com/inward/record.url?scp=84875183440&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84875183440&partnerID=8YFLogxK
U2 - 10.1016/j.snb.2013.01.096
DO - 10.1016/j.snb.2013.01.096
M3 - Article
AN - SCOPUS:84875183440
SN - 0925-4005
VL - 181
SP - 652
EP - 660
JO - Sensors and Actuators, B: Chemical
JF - Sensors and Actuators, B: Chemical
ER -