Volatilization of high molecular weight DNA by pulsed laser ablation of frozen aqueous solutions

Randall W. Nelson; Matthew J. Rainbow; Dennis E. Lohr; Peter Williams

doi:10.1126/science.2595370

Volatilization of high molecular weight DNA by pulsed laser ablation of frozen aqueous solutions

Randall W. Nelson, Matthew J. Rainbow, Dennis E. Lohr, Peter Williams

Research output: Contribution to journal › Article › peer-review

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Abstract

DNA has been volatilized by pulsed laser ablation of a thin film of a frozen aqueous DNA solution. The target film was irradiated in vacuum by a pulsed laser at power densities sufficient to ablate the ice matrix. The expanding ablated water vapor entrained embedded DNA molecules and expelled them into the gas phase. Ejection of DNA molecules as large as 410 kilodaltons was verified by collection of the ablation products and subsequent mass analysis by polyacrylamide gel electrophoresis with autoradiographic detection.

Original language	English (US)
Pages (from-to)	1585-1587
Number of pages	3
Journal	Science
Volume	246
Issue number	4937
DOIs	https://doi.org/10.1126/science.2595370
State	Published - 1989

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title = "Volatilization of high molecular weight DNA by pulsed laser ablation of frozen aqueous solutions",

abstract = "DNA has been volatilized by pulsed laser ablation of a thin film of a frozen aqueous DNA solution. The target film was irradiated in vacuum by a pulsed laser at power densities sufficient to ablate the ice matrix. The expanding ablated water vapor entrained embedded DNA molecules and expelled them into the gas phase. Ejection of DNA molecules as large as 410 kilodaltons was verified by collection of the ablation products and subsequent mass analysis by polyacrylamide gel electrophoresis with autoradiographic detection.",

author = "Nelson, {Randall W.} and Rainbow, {Matthew J.} and Lohr, {Dennis E.} and Peter Williams",

year = "1989",

doi = "10.1126/science.2595370",

language = "English (US)",

volume = "246",

pages = "1585--1587",

journal = "Science",

issn = "0036-8075",

publisher = "American Association for the Advancement of Science",

number = "4937",

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TY - JOUR

T1 - Volatilization of high molecular weight DNA by pulsed laser ablation of frozen aqueous solutions

AU - Nelson, Randall W.

AU - Rainbow, Matthew J.

AU - Lohr, Dennis E.

AU - Williams, Peter

PY - 1989

Y1 - 1989

N2 - DNA has been volatilized by pulsed laser ablation of a thin film of a frozen aqueous DNA solution. The target film was irradiated in vacuum by a pulsed laser at power densities sufficient to ablate the ice matrix. The expanding ablated water vapor entrained embedded DNA molecules and expelled them into the gas phase. Ejection of DNA molecules as large as 410 kilodaltons was verified by collection of the ablation products and subsequent mass analysis by polyacrylamide gel electrophoresis with autoradiographic detection.

AB - DNA has been volatilized by pulsed laser ablation of a thin film of a frozen aqueous DNA solution. The target film was irradiated in vacuum by a pulsed laser at power densities sufficient to ablate the ice matrix. The expanding ablated water vapor entrained embedded DNA molecules and expelled them into the gas phase. Ejection of DNA molecules as large as 410 kilodaltons was verified by collection of the ablation products and subsequent mass analysis by polyacrylamide gel electrophoresis with autoradiographic detection.

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DO - 10.1126/science.2595370

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JO - Science

JF - Science

IS - 4937

ER -

Volatilization of high molecular weight DNA by pulsed laser ablation of frozen aqueous solutions

Abstract

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