Vitamin D receptor-mediated control of Soggy, Wise, and Hairless gene expression in keratinocytes

Jui Cheng Hsieh, Rudolf C. Estess, Ichiro Kaneko, G. Kerr Whitfield, Peter Jurutka, Mark R. Haussler

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

The vitamin D receptor (VDR), but not its hormonal ligand, 1,25-dihydroxyvitamin D3 (1,25D), is required for the progression of the mammalian hair cycle.We studied three genes relevant to hair cycle signaling, DKKL1 (Soggy), SOSTDC1 (Wise), and HR (Hairless), to determine whether their expression is regulated by VDR and/or its 1,25D ligand. DKKL1 mRNA was repressed 49-72% by 1,25D in primary human and CCD-1106 KERTr keratinocytes; a functional vitamin D responsive element (VDRE) was identified at -9590 bp in murine Soggy. Similarly, SOSTDC1 mRNA was repressed 41-59% by 1,25D in KERTr and primary human keratinocytes; a functional VDRE was located at -6215 bp in human Wise. In contrast, HR mRNA was upregulated 1.56- to 2.77-fold by 1,25D in primary human and KERTr keratinocytes; a VDRE (TGGTGAgtgAGGACA) consisting of an imperfect direct repeat separated by three nucleotides (DR3) was identified at -7269 bp in the human Hairless gene that mediated dramatic induction, even in the absence of 1,25D ligand. In parallel, a DR4 thyroid hormone responsive element, TGGTGAggccAGGACA, was identified at C1304 bp in the human HR gene that conferred tri-iodothyronine (T3)-independent transcriptional activation. Because the thyroid hormone receptor controls HR expression in the CNS, whereas VDR functions in concert with the HR corepressor specifically in skin, a model is proposed wherein unliganded VDR upregulates the expression of HR, the gene product of which acts as a downstream comodulator to feedback-repress DKKL1 and SOSTDC1, resulting in integration of bone morphogenic protein and Wnt signaling to drive the mammalian hair cycle and/or influencing epidermal function.

Original languageEnglish (US)
Pages (from-to)165-178
Number of pages14
JournalJournal of Endocrinology
Volume220
Issue number2
DOIs
StatePublished - Feb 2014

Fingerprint

Calcitriol Receptors
Calcitriol
Keratinocytes
Gene Expression
Vitamin D
Hair
Ligands
Messenger RNA
Wnt Proteins
Genes
Thyroid Hormone Receptors
Co-Repressor Proteins
Nucleic Acid Repetitive Sequences
Thyroid Hormones
Transcriptional Activation
Up-Regulation
Nucleotides
Bone and Bones
Skin

Keywords

  • Gene regulation
  • Hormone receptors
  • Skin
  • Transcription factors
  • Vitamin D

ASJC Scopus subject areas

  • Endocrinology
  • Endocrinology, Diabetes and Metabolism

Cite this

Vitamin D receptor-mediated control of Soggy, Wise, and Hairless gene expression in keratinocytes. / Hsieh, Jui Cheng; Estess, Rudolf C.; Kaneko, Ichiro; Kerr Whitfield, G.; Jurutka, Peter; Haussler, Mark R.

In: Journal of Endocrinology, Vol. 220, No. 2, 02.2014, p. 165-178.

Research output: Contribution to journalArticle

Hsieh, Jui Cheng ; Estess, Rudolf C. ; Kaneko, Ichiro ; Kerr Whitfield, G. ; Jurutka, Peter ; Haussler, Mark R. / Vitamin D receptor-mediated control of Soggy, Wise, and Hairless gene expression in keratinocytes. In: Journal of Endocrinology. 2014 ; Vol. 220, No. 2. pp. 165-178.
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AU - Kerr Whitfield, G.

AU - Jurutka, Peter

AU - Haussler, Mark R.

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AB - The vitamin D receptor (VDR), but not its hormonal ligand, 1,25-dihydroxyvitamin D3 (1,25D), is required for the progression of the mammalian hair cycle.We studied three genes relevant to hair cycle signaling, DKKL1 (Soggy), SOSTDC1 (Wise), and HR (Hairless), to determine whether their expression is regulated by VDR and/or its 1,25D ligand. DKKL1 mRNA was repressed 49-72% by 1,25D in primary human and CCD-1106 KERTr keratinocytes; a functional vitamin D responsive element (VDRE) was identified at -9590 bp in murine Soggy. Similarly, SOSTDC1 mRNA was repressed 41-59% by 1,25D in KERTr and primary human keratinocytes; a functional VDRE was located at -6215 bp in human Wise. In contrast, HR mRNA was upregulated 1.56- to 2.77-fold by 1,25D in primary human and KERTr keratinocytes; a VDRE (TGGTGAgtgAGGACA) consisting of an imperfect direct repeat separated by three nucleotides (DR3) was identified at -7269 bp in the human Hairless gene that mediated dramatic induction, even in the absence of 1,25D ligand. In parallel, a DR4 thyroid hormone responsive element, TGGTGAggccAGGACA, was identified at C1304 bp in the human HR gene that conferred tri-iodothyronine (T3)-independent transcriptional activation. Because the thyroid hormone receptor controls HR expression in the CNS, whereas VDR functions in concert with the HR corepressor specifically in skin, a model is proposed wherein unliganded VDR upregulates the expression of HR, the gene product of which acts as a downstream comodulator to feedback-repress DKKL1 and SOSTDC1, resulting in integration of bone morphogenic protein and Wnt signaling to drive the mammalian hair cycle and/or influencing epidermal function.

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