TY - JOUR
T1 - Vitamin D mediates the relationship between placental cathelicidin and group B streptococcus colonization during pregnancy
AU - Akoh, Christine C.
AU - Pressman, Eva K.
AU - Whisner, Corrie M.
AU - Thomas, Carrie
AU - Cao, Chang
AU - Kent, Tera
AU - Cooper, Elizabeth
AU - O'Brien, Kimberly O.
N1 - Funding Information:
This work that was supported by the National Institute of Food and Agriculture (NIFA), U.S. Department of Agriculture [2005-35200-15218, 2012-67017-30216] and the National Center for Research Resources (NCRR) [UL1 RR 024160]. The funding sources had no involvement in preparing the manuscript.
Publisher Copyright:
© 2017 Elsevier B.V.
PY - 2017/6
Y1 - 2017/6
N2 - Vitamin D is thought to modulate innate immune responses, and recent studies have highlighted the autocrine and paracrine functions of vitamin D in the placenta. Our objective was to determine the relationship between maternal vitamin D status and placental antimicrobial peptide (AMP) expression in a group of racially and ethnically diverse pregnant adolescents. In this study, 158 pregnant adolescents were recruited from the Rochester Adolescent Maternity Program (RAMP) in Rochester, NY. Maternal serum concentrations of the vitamin D biomarkers, 25-hydroxyvitamin D (25(OH)D) and 1,25-dihydroxyvitamin D (1,25(OH)2D), were measured at mid-gestation (∼26 weeks) and at delivery. At the placental level, vitamin D regulatory proteins (cubilin, megalin, 1α-hydroxylase (CYP27B1), 24-hydroxylase (CYP24A1), vitamin D receptor (VDR)) and AMPs (cathelicidin and hepcidin) were analyzed using quantitative PCR and western blot techniques. Placental CYP27B1 mRNA expression was significantly positively associated with both placental cathelicidin mRNA expression (P < 0.0001) and placental hepcidin mRNA expression (P = 0.002). In teens with positive recto-vaginal group B streptococcus (GBS) colonization, placental mRNA expression of cathelicidin (P = 0.007), cubilin (P = 0.03), and CYP27B1 (P = 0.04) were significantly lower compared to those who tested negative for this infection. A mediation analysis showed that the indirect relationship between GBS colonization and placental cathelicidin mRNA expression was mediated by the placental mRNA expression of the vitamin D proteins cubilin and CYP27B1 (P = 0.02). Additional research is needed to identify the role and relative contributions of placental and systemic vitamin D metabolites in relation to potentially pathogenic microorganisms which may be present during pregnancy.
AB - Vitamin D is thought to modulate innate immune responses, and recent studies have highlighted the autocrine and paracrine functions of vitamin D in the placenta. Our objective was to determine the relationship between maternal vitamin D status and placental antimicrobial peptide (AMP) expression in a group of racially and ethnically diverse pregnant adolescents. In this study, 158 pregnant adolescents were recruited from the Rochester Adolescent Maternity Program (RAMP) in Rochester, NY. Maternal serum concentrations of the vitamin D biomarkers, 25-hydroxyvitamin D (25(OH)D) and 1,25-dihydroxyvitamin D (1,25(OH)2D), were measured at mid-gestation (∼26 weeks) and at delivery. At the placental level, vitamin D regulatory proteins (cubilin, megalin, 1α-hydroxylase (CYP27B1), 24-hydroxylase (CYP24A1), vitamin D receptor (VDR)) and AMPs (cathelicidin and hepcidin) were analyzed using quantitative PCR and western blot techniques. Placental CYP27B1 mRNA expression was significantly positively associated with both placental cathelicidin mRNA expression (P < 0.0001) and placental hepcidin mRNA expression (P = 0.002). In teens with positive recto-vaginal group B streptococcus (GBS) colonization, placental mRNA expression of cathelicidin (P = 0.007), cubilin (P = 0.03), and CYP27B1 (P = 0.04) were significantly lower compared to those who tested negative for this infection. A mediation analysis showed that the indirect relationship between GBS colonization and placental cathelicidin mRNA expression was mediated by the placental mRNA expression of the vitamin D proteins cubilin and CYP27B1 (P = 0.02). Additional research is needed to identify the role and relative contributions of placental and systemic vitamin D metabolites in relation to potentially pathogenic microorganisms which may be present during pregnancy.
KW - Antimicrobials
KW - Infections
KW - Placenta
KW - Pregnancy
KW - Vitamin D
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U2 - 10.1016/j.jri.2017.06.001
DO - 10.1016/j.jri.2017.06.001
M3 - Article
C2 - 28622535
AN - SCOPUS:85020706893
SN - 0165-0378
VL - 121
SP - 42
EP - 48
JO - Journal of Reproductive Immunology
JF - Journal of Reproductive Immunology
ER -