Visualizing macromolecular complexes with in situ liquid scanning transmission electron microscopy

James E. Evans; Katherine L. Jungjohann; Peony C.K. Wong; Po Lin Chiu; Gavin H. Dutrow; Ilke Arslan; Nigel D. Browning

doi:10.1016/j.micron.2012.01.018

Visualizing macromolecular complexes with in situ liquid scanning transmission electron microscopy

James E. Evans, Katherine L. Jungjohann, Peony C.K. Wong, Po Lin Chiu, Gavin H. Dutrow, Ilke Arslan, Nigel D. Browning

Research output: Contribution to journal › Article › peer-review

72 Scopus citations

Abstract

A central focus of biological research is understanding the structure/function relationship of macromolecular protein complexes. Yet conventional transmission electron microscopy techniques are limited to static observations. Here we present the first direct images of purified macromolecular protein complexes using in situ liquid scanning transmission electron microscopy. Our results establish the capability of this technique for visualizing the interface between biology and nanotechnology with high fidelity while also probing the interactions of biomolecules within solution. This method represents an important advancement towards allowing future high-resolution observations of biological processes and conformational dynamics in real-time.

Original language	English (US)
Pages (from-to)	1085-1090
Number of pages	6
Journal	Micron
Volume	43
Issue number	11
DOIs	https://doi.org/10.1016/j.micron.2012.01.018
State	Published - Nov 2012
Externally published	Yes

Keywords

Aberration corrected STEM
Cryo-EM
In situ TEM
Liquid STEM
NLP
Nanolipoprotein
STEM
TEM

ASJC Scopus subject areas

Structural Biology
Cell Biology

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10.1016/j.micron.2012.01.018

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title = "Visualizing macromolecular complexes with in situ liquid scanning transmission electron microscopy",

abstract = "A central focus of biological research is understanding the structure/function relationship of macromolecular protein complexes. Yet conventional transmission electron microscopy techniques are limited to static observations. Here we present the first direct images of purified macromolecular protein complexes using in situ liquid scanning transmission electron microscopy. Our results establish the capability of this technique for visualizing the interface between biology and nanotechnology with high fidelity while also probing the interactions of biomolecules within solution. This method represents an important advancement towards allowing future high-resolution observations of biological processes and conformational dynamics in real-time.",

keywords = "Aberration corrected STEM, Cryo-EM, In situ TEM, Liquid STEM, NLP, Nanolipoprotein, STEM, TEM",

author = "Evans, {James E.} and Jungjohann, {Katherine L.} and Wong, {Peony C.K.} and Chiu, {Po Lin} and Dutrow, {Gavin H.} and Ilke Arslan and Browning, {Nigel D.}",

note = "Funding Information: J.E.E. and N.D.B. acknowledge NIH funding support from grant number 5RC1GM091755 . A portion of this work was performed at the Pacific Northwest National Laboratory which is operated by Battelle Memorial Institute for the U.S. Department of Energy under Contract No. DE-AC05-76RL01830.",

year = "2012",

month = nov,

doi = "10.1016/j.micron.2012.01.018",

language = "English (US)",

volume = "43",

pages = "1085--1090",

journal = "Micron",

issn = "0968-4328",

publisher = "Elsevier Limited",

number = "11",

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TY - JOUR

T1 - Visualizing macromolecular complexes with in situ liquid scanning transmission electron microscopy

AU - Evans, James E.

AU - Jungjohann, Katherine L.

AU - Wong, Peony C.K.

AU - Chiu, Po Lin

AU - Dutrow, Gavin H.

AU - Arslan, Ilke

AU - Browning, Nigel D.

N1 - Funding Information: J.E.E. and N.D.B. acknowledge NIH funding support from grant number 5RC1GM091755 . A portion of this work was performed at the Pacific Northwest National Laboratory which is operated by Battelle Memorial Institute for the U.S. Department of Energy under Contract No. DE-AC05-76RL01830.

PY - 2012/11

Y1 - 2012/11

N2 - A central focus of biological research is understanding the structure/function relationship of macromolecular protein complexes. Yet conventional transmission electron microscopy techniques are limited to static observations. Here we present the first direct images of purified macromolecular protein complexes using in situ liquid scanning transmission electron microscopy. Our results establish the capability of this technique for visualizing the interface between biology and nanotechnology with high fidelity while also probing the interactions of biomolecules within solution. This method represents an important advancement towards allowing future high-resolution observations of biological processes and conformational dynamics in real-time.

AB - A central focus of biological research is understanding the structure/function relationship of macromolecular protein complexes. Yet conventional transmission electron microscopy techniques are limited to static observations. Here we present the first direct images of purified macromolecular protein complexes using in situ liquid scanning transmission electron microscopy. Our results establish the capability of this technique for visualizing the interface between biology and nanotechnology with high fidelity while also probing the interactions of biomolecules within solution. This method represents an important advancement towards allowing future high-resolution observations of biological processes and conformational dynamics in real-time.

KW - Aberration corrected STEM

KW - Cryo-EM

KW - In situ TEM

KW - Liquid STEM

KW - NLP

KW - Nanolipoprotein

KW - STEM

KW - TEM

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DO - 10.1016/j.micron.2012.01.018

M3 - Article

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SN - 0968-4328

VL - 43

SP - 1085

EP - 1090

JO - Micron

JF - Micron

IS - 11

ER -

Visualizing macromolecular complexes with in situ liquid scanning transmission electron microscopy

Abstract

Keywords

ASJC Scopus subject areas

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