Visualizing macromolecular complexes with in situ liquid scanning transmission electron microscopy

James E. Evans, Katherine L. Jungjohann, Peony C.K. Wong, Po Lin Chiu, Gavin H. Dutrow, Ilke Arslan, Nigel D. Browning

Research output: Contribution to journalArticle

57 Scopus citations

Abstract

A central focus of biological research is understanding the structure/function relationship of macromolecular protein complexes. Yet conventional transmission electron microscopy techniques are limited to static observations. Here we present the first direct images of purified macromolecular protein complexes using in situ liquid scanning transmission electron microscopy. Our results establish the capability of this technique for visualizing the interface between biology and nanotechnology with high fidelity while also probing the interactions of biomolecules within solution. This method represents an important advancement towards allowing future high-resolution observations of biological processes and conformational dynamics in real-time.

Original languageEnglish (US)
Pages (from-to)1085-1090
Number of pages6
JournalMicron
Volume43
Issue number11
DOIs
StatePublished - Nov 1 2012
Externally publishedYes

Keywords

  • Aberration corrected STEM
  • Cryo-EM
  • In situ TEM
  • Liquid STEM
  • NLP
  • Nanolipoprotein
  • STEM
  • TEM

ASJC Scopus subject areas

  • Structural Biology
  • Materials Science(all)
  • Physics and Astronomy(all)
  • Cell Biology

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  • Cite this

    Evans, J. E., Jungjohann, K. L., Wong, P. C. K., Chiu, P. L., Dutrow, G. H., Arslan, I., & Browning, N. D. (2012). Visualizing macromolecular complexes with in situ liquid scanning transmission electron microscopy. Micron, 43(11), 1085-1090. https://doi.org/10.1016/j.micron.2012.01.018