Visualizing macromolecular complexes with in situ liquid scanning transmission electron microscopy

James E. Evans, Katherine L. Jungjohann, Peony C K Wong, Po-Lin Chiu, Gavin H. Dutrow, Ilke Arslan, Nigel D. Browning

Research output: Contribution to journalArticle

51 Citations (Scopus)

Abstract

A central focus of biological research is understanding the structure/function relationship of macromolecular protein complexes. Yet conventional transmission electron microscopy techniques are limited to static observations. Here we present the first direct images of purified macromolecular protein complexes using in situ liquid scanning transmission electron microscopy. Our results establish the capability of this technique for visualizing the interface between biology and nanotechnology with high fidelity while also probing the interactions of biomolecules within solution. This method represents an important advancement towards allowing future high-resolution observations of biological processes and conformational dynamics in real-time.

Original languageEnglish (US)
Pages (from-to)1085-1090
Number of pages6
JournalMicron
Volume43
Issue number11
DOIs
StatePublished - Nov 2012
Externally publishedYes

Fingerprint

Macromolecular Substances
Multiprotein Complexes
Scanning Transmission Electron Microscopy
Biological Phenomena
Nanotechnology
Transmission Electron Microscopy
Research

Keywords

  • Aberration corrected STEM
  • Cryo-EM
  • In situ TEM
  • Liquid STEM
  • Nanolipoprotein
  • NLP
  • STEM
  • TEM

ASJC Scopus subject areas

  • Cell Biology
  • Structural Biology

Cite this

Evans, J. E., Jungjohann, K. L., Wong, P. C. K., Chiu, P-L., Dutrow, G. H., Arslan, I., & Browning, N. D. (2012). Visualizing macromolecular complexes with in situ liquid scanning transmission electron microscopy. Micron, 43(11), 1085-1090. https://doi.org/10.1016/j.micron.2012.01.018

Visualizing macromolecular complexes with in situ liquid scanning transmission electron microscopy. / Evans, James E.; Jungjohann, Katherine L.; Wong, Peony C K; Chiu, Po-Lin; Dutrow, Gavin H.; Arslan, Ilke; Browning, Nigel D.

In: Micron, Vol. 43, No. 11, 11.2012, p. 1085-1090.

Research output: Contribution to journalArticle

Evans, JE, Jungjohann, KL, Wong, PCK, Chiu, P-L, Dutrow, GH, Arslan, I & Browning, ND 2012, 'Visualizing macromolecular complexes with in situ liquid scanning transmission electron microscopy', Micron, vol. 43, no. 11, pp. 1085-1090. https://doi.org/10.1016/j.micron.2012.01.018
Evans, James E. ; Jungjohann, Katherine L. ; Wong, Peony C K ; Chiu, Po-Lin ; Dutrow, Gavin H. ; Arslan, Ilke ; Browning, Nigel D. / Visualizing macromolecular complexes with in situ liquid scanning transmission electron microscopy. In: Micron. 2012 ; Vol. 43, No. 11. pp. 1085-1090.
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