Visualizing a protein quake with time-resolved X-ray scattering at a free-electron laser

David Arnlund, Linda C. Johansson, Cecilia Wickstrand, Anton Barty, Garth J. Williams, Erik Malmerberg, Jan Davidsson, Despina Milathianaki, Daniel P. DePonte, Robert L. Shoeman, Dingjie Wang, Daniel James, Gergely Katona, Sebastian Westenhoff, Thomas A. White, Andrew Aquila, Sadia Bari, Peter Berntsen, Mike Bogan, Tim Brandt Van Driel & 26 others R. Bruce Doak, Kasper Skov Kjær, Matthias Frank, Raimund Fromme, Ingo Grotjohann, Robert Henning, Mark S. Hunter, Richard Kirian, Irina Kosheleva, Christopher Kupitz, Mengning Liang, Andrew V. Martin, Martin Meedom Nielsen, Marc Messerschmidt, M. Marvin Seibert, Jennie Sjöhamn, Francesco Stellato, Uwe Weierstall, Nadia Zatsepin, John Spence, Petra Fromme, Ilme Schlichting, Sébastien Boutet, Gerrit Groenhof, Henry N. Chapman, Richard Neutze

Research output: Contribution to journalArticle

105 Citations (Scopus)

Abstract

We describe a method to measure ultrafast protein structural changes using time-resolved wide-angle X-ray scattering at an X-ray free-electron laser. We demonstrated this approach using multiphoton excitation of the Blastochloris viridis photosynthetic reaction center, observing an ultrafast global conformational change that arises within picoseconds and precedes the propagation of heat through the protein. This provides direct structural evidence for a 'protein quake': the hypothesis that proteins rapidly dissipate energy through quake-like structural motions.

Original languageEnglish (US)
Pages (from-to)923-926
Number of pages4
JournalNature Methods
Volume11
Issue number9
DOIs
StatePublished - 2014

Fingerprint

Free electron lasers
X ray scattering
Lasers
X-Rays
Electrons
Proteins
Photosynthetic Reaction Center Complex Proteins
X ray lasers
Hot Temperature

ASJC Scopus subject areas

  • Biotechnology
  • Molecular Biology
  • Biochemistry
  • Cell Biology
  • Medicine(all)

Cite this

Arnlund, D., Johansson, L. C., Wickstrand, C., Barty, A., Williams, G. J., Malmerberg, E., ... Neutze, R. (2014). Visualizing a protein quake with time-resolved X-ray scattering at a free-electron laser. Nature Methods, 11(9), 923-926. https://doi.org/10.1038/nmeth.3067

Visualizing a protein quake with time-resolved X-ray scattering at a free-electron laser. / Arnlund, David; Johansson, Linda C.; Wickstrand, Cecilia; Barty, Anton; Williams, Garth J.; Malmerberg, Erik; Davidsson, Jan; Milathianaki, Despina; DePonte, Daniel P.; Shoeman, Robert L.; Wang, Dingjie; James, Daniel; Katona, Gergely; Westenhoff, Sebastian; White, Thomas A.; Aquila, Andrew; Bari, Sadia; Berntsen, Peter; Bogan, Mike; Van Driel, Tim Brandt; Doak, R. Bruce; Kjær, Kasper Skov; Frank, Matthias; Fromme, Raimund; Grotjohann, Ingo; Henning, Robert; Hunter, Mark S.; Kirian, Richard; Kosheleva, Irina; Kupitz, Christopher; Liang, Mengning; Martin, Andrew V.; Nielsen, Martin Meedom; Messerschmidt, Marc; Seibert, M. Marvin; Sjöhamn, Jennie; Stellato, Francesco; Weierstall, Uwe; Zatsepin, Nadia; Spence, John; Fromme, Petra; Schlichting, Ilme; Boutet, Sébastien; Groenhof, Gerrit; Chapman, Henry N.; Neutze, Richard.

In: Nature Methods, Vol. 11, No. 9, 2014, p. 923-926.

Research output: Contribution to journalArticle

Arnlund, D, Johansson, LC, Wickstrand, C, Barty, A, Williams, GJ, Malmerberg, E, Davidsson, J, Milathianaki, D, DePonte, DP, Shoeman, RL, Wang, D, James, D, Katona, G, Westenhoff, S, White, TA, Aquila, A, Bari, S, Berntsen, P, Bogan, M, Van Driel, TB, Doak, RB, Kjær, KS, Frank, M, Fromme, R, Grotjohann, I, Henning, R, Hunter, MS, Kirian, R, Kosheleva, I, Kupitz, C, Liang, M, Martin, AV, Nielsen, MM, Messerschmidt, M, Seibert, MM, Sjöhamn, J, Stellato, F, Weierstall, U, Zatsepin, N, Spence, J, Fromme, P, Schlichting, I, Boutet, S, Groenhof, G, Chapman, HN & Neutze, R 2014, 'Visualizing a protein quake with time-resolved X-ray scattering at a free-electron laser', Nature Methods, vol. 11, no. 9, pp. 923-926. https://doi.org/10.1038/nmeth.3067
Arnlund D, Johansson LC, Wickstrand C, Barty A, Williams GJ, Malmerberg E et al. Visualizing a protein quake with time-resolved X-ray scattering at a free-electron laser. Nature Methods. 2014;11(9):923-926. https://doi.org/10.1038/nmeth.3067
Arnlund, David ; Johansson, Linda C. ; Wickstrand, Cecilia ; Barty, Anton ; Williams, Garth J. ; Malmerberg, Erik ; Davidsson, Jan ; Milathianaki, Despina ; DePonte, Daniel P. ; Shoeman, Robert L. ; Wang, Dingjie ; James, Daniel ; Katona, Gergely ; Westenhoff, Sebastian ; White, Thomas A. ; Aquila, Andrew ; Bari, Sadia ; Berntsen, Peter ; Bogan, Mike ; Van Driel, Tim Brandt ; Doak, R. Bruce ; Kjær, Kasper Skov ; Frank, Matthias ; Fromme, Raimund ; Grotjohann, Ingo ; Henning, Robert ; Hunter, Mark S. ; Kirian, Richard ; Kosheleva, Irina ; Kupitz, Christopher ; Liang, Mengning ; Martin, Andrew V. ; Nielsen, Martin Meedom ; Messerschmidt, Marc ; Seibert, M. Marvin ; Sjöhamn, Jennie ; Stellato, Francesco ; Weierstall, Uwe ; Zatsepin, Nadia ; Spence, John ; Fromme, Petra ; Schlichting, Ilme ; Boutet, Sébastien ; Groenhof, Gerrit ; Chapman, Henry N. ; Neutze, Richard. / Visualizing a protein quake with time-resolved X-ray scattering at a free-electron laser. In: Nature Methods. 2014 ; Vol. 11, No. 9. pp. 923-926.
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