Viruses representing two new genomovirus species identified in citrus from Tunisia

Camila Chabi-Jesus, Asma Najar, Rafaela S. Fontenele, Safaa G. Kumari, Pedro Luis Ramos-González, Juliana Freitas-Astúa, Simona Kraberger, Arvind Varsani

Research output: Contribution to journalArticle

Abstract

Using a high-throughput sequencing approach, we identified four genomoviruses (family Genomoviridae) associated with a sweet orange (Citrus sinensis) plant collected in Tunisia. The ssDNA genomes of these genomoviruses, which were amplified, cloned and Sanger sequenced, range in size from 2156 to 2191 nt. Three of these viruses share > 99% full-genome pairwise sequence identity and are referred to as citrus Tunisia genomovirus 1 (CTNGmV-1). The CTNGmV-1 isolates share < 62% genome-wide pairwise nucleotide sequence identity with other genomoviruses and belong to the genus Gemykolovirus. The genome of the fourth virus, which was called CTNGmV-2, shares < 68% nucleotide sequence identity with other genomoviruses and belongs to the genus Gemycircularvirus. Based on the species demarcation criteria for members of the family Genomoviridae, CTNGmV-1 and -2 would each represent a new species. Although found associated with Citrus sp. plants, it is likely that these viruses infect fungi or other organisms associated with the plants.

Original languageEnglish (US)
Pages (from-to)1225-1229
Number of pages5
JournalArchives of virology
Volume165
Issue number5
DOIs
StatePublished - May 1 2020

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ASJC Scopus subject areas

  • Virology

Cite this

Chabi-Jesus, C., Najar, A., Fontenele, R. S., Kumari, S. G., Ramos-González, P. L., Freitas-Astúa, J., Kraberger, S., & Varsani, A. (2020). Viruses representing two new genomovirus species identified in citrus from Tunisia. Archives of virology, 165(5), 1225-1229. https://doi.org/10.1007/s00705-020-04569-8