Virulence of Salmonella typhimurium mutants for White Leghorn chicks.

S. B. Porter, S. A. Tinge, R. Curtiss

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

A strain of Salmonella typhimurium that is highly virulent for 1-day-old white leghorn chicks was genetically modified by deletion (delta) of the adenylate cyclase (cya) and cyclic AMP receptor protein (crp) genes or by removal (curing) of the 91-kilobase virulence plasmid. These mutants were then compared with the wild-type S. typhimurium strain for virulence in 1-day-old chicks and for their ability to colonize chicks of various ages. The plasmid-cured mutant showed a slight reduction in virulence, whereas the delta cya delta crp mutant was completely avirulent. The wild-type strain and both mutant strains were capable of colonizing various organs within the chicks. At all time points, the delta cya delta crp strain colonized chicks at lower levels than the wild-type strain. Titers of the plasmid-cured strain increased more slowly in visceral organs than did those of the wild type.

Original languageEnglish (US)
Pages (from-to)265-273
Number of pages9
JournalAvian Diseases
Volume37
Issue number2
StatePublished - Apr 1993
Externally publishedYes

Fingerprint

Cyclic AMP Receptor Protein
Salmonella typhimurium
White Leghorn
Salmonella Typhimurium
Virulence
Plasmids
virulence
chicks
mutants
cyclic AMP
plasmids
Adenylyl Cyclases
receptors
plasmid curing
adenylate cyclase
proteins
Genes

ASJC Scopus subject areas

  • Cancer Research
  • Immunology
  • veterinary(all)

Cite this

Porter, S. B., Tinge, S. A., & Curtiss, R. (1993). Virulence of Salmonella typhimurium mutants for White Leghorn chicks. Avian Diseases, 37(2), 265-273.

Virulence of Salmonella typhimurium mutants for White Leghorn chicks. / Porter, S. B.; Tinge, S. A.; Curtiss, R.

In: Avian Diseases, Vol. 37, No. 2, 04.1993, p. 265-273.

Research output: Contribution to journalArticle

Porter, SB, Tinge, SA & Curtiss, R 1993, 'Virulence of Salmonella typhimurium mutants for White Leghorn chicks.', Avian Diseases, vol. 37, no. 2, pp. 265-273.
Porter SB, Tinge SA, Curtiss R. Virulence of Salmonella typhimurium mutants for White Leghorn chicks. Avian Diseases. 1993 Apr;37(2):265-273.
Porter, S. B. ; Tinge, S. A. ; Curtiss, R. / Virulence of Salmonella typhimurium mutants for White Leghorn chicks. In: Avian Diseases. 1993 ; Vol. 37, No. 2. pp. 265-273.
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