Virulence and vaccine potential of phoP mutants of Salmonella typhimurium

Jorge E. Galán, Roy Curtiss

Research output: Contribution to journalArticle

150 Citations (Scopus)

Abstract

We have constructed Salmonella typhimurium phoP mutants and found them to be avirulent and able to induce a protective immune response. BALB/c mice survived challenge with phoP derivatives of the highly virulent S. typhimurium strains SR-11 and SL1344 when inoculated intraperitoneally and per oral with doses equivalent to 104 50% lethal doses (LD50) of the parent virulent strains. The avirulent mutants were able to establish an infection of the Peyer's patches of orally infected animals for up to 10 days after inoculation but were very inefficient at reaching the spleens. Despite the low level of infectivity of these mutants, immunized animals developed a delayed-type hypersensitivity (DTH) response to Salmonella antigens and resisted challenge with up to 104 LD50 of the virulent parent strain 30 days after immunization.

Original languageEnglish (US)
Pages (from-to)433-443
Number of pages11
JournalMicrobial Pathogenesis
Volume6
Issue number6
DOIs
StatePublished - 1989
Externally publishedYes

Fingerprint

Lethal Dose 50
Salmonella typhimurium
Virulence
Vaccines
Peyer's Patches
Delayed Hypersensitivity
Salmonella
Immunization
Spleen
Antigens
Infection

Keywords

  • avirulence
  • delayed-type hypersensitivity (DTH)
  • global regulation
  • immunogenicity
  • phoP
  • Salmonella pathogenesis
  • Salmonella typhimurium

ASJC Scopus subject areas

  • Microbiology
  • Infectious Diseases

Cite this

Virulence and vaccine potential of phoP mutants of Salmonella typhimurium. / Galán, Jorge E.; Curtiss, Roy.

In: Microbial Pathogenesis, Vol. 6, No. 6, 1989, p. 433-443.

Research output: Contribution to journalArticle

Galán, Jorge E. ; Curtiss, Roy. / Virulence and vaccine potential of phoP mutants of Salmonella typhimurium. In: Microbial Pathogenesis. 1989 ; Vol. 6, No. 6. pp. 433-443.
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