Viral transgenesis of embryonic cell cultures from the freshwater microcrustacean Daphnia

Christy D. Robinson, Sebastian Lourido, Sean P. Whelan, Jeffry L. Dudycha, Michael Lynch, Sharon Isern

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

The aim of this study was to determine whether a recombinant vesicular stomatitis virus (VSV) vector encoding a transgene could be used to infect and express a foreign gene in embryonic primary cell cultures derived from the freshwater microcrustacean Daphnia, the most widely used ecotoxicological model organism. To facilitate the evaluation of gene transfer, a reproducible method for establishing primary cultures from Daphnia embryonic tissues was developed. Within 24 hr after infection, transgene expression could be detected in cell culture. VSV was found to replicate in the cells with no apparent cytopathic effect. Here we report the first evidence of gene transfer and foreign gene expression in cultures of Daphnia embryonic cells using a recombinant viral vector. copy; 2005 Wiley-Liss, Inc.

Original languageEnglish (US)
Pages (from-to)62-67
Number of pages6
JournalJournal of Experimental Zoology Part A: Comparative Experimental Biology
Volume305 A
Issue number1
DOIs
StatePublished - Jan 1 2006
Externally publishedYes

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Daphnia
Vesiculovirus
cell culture
gene transfer
transgenes
cytopathogenicity
cells
gene expression
organisms
infection
transgenesis
genes
methodology

ASJC Scopus subject areas

  • Animal Science and Zoology

Cite this

Viral transgenesis of embryonic cell cultures from the freshwater microcrustacean Daphnia. / Robinson, Christy D.; Lourido, Sebastian; Whelan, Sean P.; Dudycha, Jeffry L.; Lynch, Michael; Isern, Sharon.

In: Journal of Experimental Zoology Part A: Comparative Experimental Biology, Vol. 305 A, No. 1, 01.01.2006, p. 62-67.

Research output: Contribution to journalArticle

Robinson, Christy D. ; Lourido, Sebastian ; Whelan, Sean P. ; Dudycha, Jeffry L. ; Lynch, Michael ; Isern, Sharon. / Viral transgenesis of embryonic cell cultures from the freshwater microcrustacean Daphnia. In: Journal of Experimental Zoology Part A: Comparative Experimental Biology. 2006 ; Vol. 305 A, No. 1. pp. 62-67.
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