Vertical profiles of methanogenesis and methanogens in two contrasting acidic peatlands in central New York State, USA

Hinsby Cadillo-Quiroz, Suzanna Bräuer, Erika Yashiro, Christine Sun, Joseph Yavitt, Stephen Zinder

Research output: Contribution to journalArticle

120 Citations (Scopus)

Abstract

Northern acidic peatlands are important sources of atmospheric methane, yet the methanogens in them are poorly characterized. We examined methanogenic activities and methanogen populations at different depths in two peatlands, McLean bog (MB) and Chicago bog (CB). Both have acidic (pH 3.5-4.5) peat soils, but the pH of the deeper layers of CB is near-neutral, reflecting its previous existence as a neutral-pH fen. Acetotrophic and hydrogenotrophic methanogenesis could be stimulated in upper samples from both bogs, and phylotypes of methanogens using H2/CO2 (Methanomicrobiales) or acetate (Methanosarcinales) were identified in 16S rRNA gene clone libraries and by terminal restriction fragment length polymorphism (T-RFLP) analyses using a novel primer/restriction enzyme set that we developed. Particularly dominant in the upper layers was a clade in the Methanomicrobiales, called E2 here and the R10 or fen group elsewhere, estimated by quantitative polymerase chain reaction to be present at ∼108 cells per gram of dry peat. Methanogenic activity was considerably lower in deeper samples from both bogs. The methanogen populations detected by T-RFLP in deeper portions of MB were mainly E2 and the uncultured euryarchaeal rice cluster (RC)-II group, whereas populations in the less acidic CB deep layers were considerably different, and included a Methanomicrobiales clade we call E1-E1′, as well as RC-I, RC-II, marine benthic group D, and a new cluster that we call the subaqueous cluster. E2 was barely detectable in the deeper samples from CB, further evidence for the associations of most organisms in this group with acidic habitats.

Original languageEnglish (US)
Pages (from-to)1428-1440
Number of pages13
JournalEnvironmental Microbiology
Volume8
Issue number8
DOIs
StatePublished - Aug 2006
Externally publishedYes

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methanogens
Wetlands
methanogenesis
bogs
peatlands
bog
methane production
peatland
vertical profile
Methanomicrobiales
Soil
rice
fens
fen
Restriction Fragment Length Polymorphisms
Methanosarcinales
restriction fragment length polymorphism
polymorphism
peat soils
peat soil

ASJC Scopus subject areas

  • Environmental Science(all)
  • Microbiology
  • Applied Microbiology and Biotechnology

Cite this

Vertical profiles of methanogenesis and methanogens in two contrasting acidic peatlands in central New York State, USA. / Cadillo-Quiroz, Hinsby; Bräuer, Suzanna; Yashiro, Erika; Sun, Christine; Yavitt, Joseph; Zinder, Stephen.

In: Environmental Microbiology, Vol. 8, No. 8, 08.2006, p. 1428-1440.

Research output: Contribution to journalArticle

Cadillo-Quiroz, Hinsby ; Bräuer, Suzanna ; Yashiro, Erika ; Sun, Christine ; Yavitt, Joseph ; Zinder, Stephen. / Vertical profiles of methanogenesis and methanogens in two contrasting acidic peatlands in central New York State, USA. In: Environmental Microbiology. 2006 ; Vol. 8, No. 8. pp. 1428-1440.
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